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mmu_circFbh1_866
Basic information of circular RNA
PanCircBase ID:
mmu_circFbh1_866
mm39_Chr. Position ID:
chr2|11751870|11760588|-
Gene name:
Fbh1
Transcript:
ENSMUST00000071564.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
19,18,17,16,15,14,13
Exon/intron offsets:
0,1304,1877,2471,4391,4607,8581
Genomic length:
8718
Exon/intron sizes in nt:
1,07,12,61,98,76,12,21,00,000
Flanking exon/introns:
chr2:11748568-11751870|chr2:11760588-11761227
Splice length:
866
Other database IDs:
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_128752
Human Ortholog IDs:
Mature spliced Sequence:
CTATCATGAACATAGTTCTGTCTCAGCCATGTGGGAAGATCTTTGTAGGGGACCCCCACCAGCAGATCTATACCTTCCGAGGTGCAGTGAATGCTCTGTTCACAGTTCCCCACACCCACGTCTTCTATCTCACACAGAGTTTTAGATTTGGTGTGGAAATAGCCTATGTGGGAGCTACCATTTTGGATGTCTGCAAGAGAGTCAGGAAGAAGACGTTGGTTGGAGGGAACCATCAGAGTGGCATAAGAGGTGACATAAAGGGACAAGTAGCTCTGCTGTCCAGGACCAATGCCAATGTGTTTGACGAGGCTGTCCGGGTAACAGAAGGAGAATCCCCTGCAAGGATACATTTGATTGGGGGGATTAAATCGTTTGGATTGGACAGAATCATTGACATTTGGACCCTTCTTCAGCCAGAGGAAGAACGGAGGAAACGAGACCTCATCATTAAAGACAGGTTCATCAGAAGATGGGTGCATAAAGAAGGCTTCAGTGGCTTCAAGAGGTATGTGACTGCTGCCGAGGACAAGGAACTGGAAGCGAAGATTGCGGTAGTTGAAAAATACAATATCAGGATTCCAGAACTGGTAGAAAGGATAGAGAGATGCCACATAGACGATTTGGACTTTGCAGAGTACATTTTGGGCACCGTGCACAAAGCCAAAGGGTTAGAGTTTGACACTGTGCACGTTTTGGATGATTTCGTGAAAGTGCCTTGTGCCAGGCATAATCTTGCCCAGCTTCCTCACTTCAGAGTTGAGTCGTTTTCTGAGGATGAATGGAATTTACTGTATGTTGCTGTTACTCGTGCCAAGAAGCGGCTCATAATGACCAAATCCCTGGAGAACATCCTGACTCTGGCTGGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGTTGCTGTTACTCGTGCCA
60.18
ATAGATCTGCTGGTGGGGGT
60.03
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCTGTTACTCGTGCCAAGA
59.61
ATAGATCTGCTGGTGGGGGT
60.03
141
PCR template sequence:
TTCTGAGGATGAATGGAATTTACTGTATGTTGCTGTTACTCGTGCCAAGAAGCGGCTCATAATGACCAAATCCCTGGAGAACATCCTGACTCTGGCTGGGCTATCATGAACATAGTTCTGTCTCAGCCATGTGGGAAGATCTTTGTAGGGGACCCCCACCAGCAGATCTATACCTTCCGAGGTGCAGTGAATGCTCTGTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-188-3p(2), mmu-miR-31-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-181b-5p(1), mmu-miR-96-5p(1), mmu-miR-194-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GACTCTGGCTGGGCTATCATGAACAT
siRNA sets
ATGTTCATGATAGCCCAGC, TGTTCATGATAGCCCAGCC, TTCATGATAGCCCAGCCAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFBH1_866_ORF_1
1.128
0.180396792
0.988295847
954
ORF sequence:
ATGGAATTTACTGTATGTTGCTGTTACTCGTGCCAAGAAGCGGCTCATAATGACCAAATCCCTGGAGAACATCCTGACTCTGGCTGGGCTATCATGAACATAGTTCTGTCTCAGCCATGTGGGAAGATCTTTGTAGGGGACCCCCACCAGCAGATCTATACCTTCCGAGGTGCAGTGAATGCTCTGTTCACAGTTCCCCACACCCACGTCTTCTATCTCACACAGAGTTTTAGATTTGGTGTGGAAATAGCCTATGTGGGAGCTACCATTTTGGATGTCTGCAAGAGAGTCAGGAAGAAGACGTTGGTTGGAGGGAACCATCAGAGTGGCATAAGAGGTGACATAAAGGGACAAGTAGCTCTGCTGTCCAGGACCAATGCCAATGTGTTTGACGAGGCTGTCCGGGTAACAGAAGGAGAATCCCCTGCAAGGATACATTTGATTGGGGGGATTAAATCGTTTGGATTGGACAGAATCATTGACATTTGGACCCTTCTTCAGCCAGAGGAAGAACGGAGGAAACGAGACCTCATCATTAAAGACAGGTTCATCAGAAGATGGGTGCATAAAGAAGGCTTCAGTGGCTTCAAGAGGTATGTGACTGCTGCCGAGGACAAGGAACTGGAAGCGAAGATTGCGGTAGTTGAAAAATACAATATCAGGATTCCAGAACTGGTAGAAAGGATAGAGAGATGCCACATAGACGATTTGGACTTTGCAGAGTACATTTTGGGCACCGTGCACAAAGCCAAAGGGTTAGAGTTTGACACTGTGCACGTTTTGGATGATTTCGTGAAAGTGCCTTGTGCCAGGCATAATCTTGCCCAGCTTCCTCACTTCAGAGTTGAGTCGTTTTCTGAGGATGAATGGAATTTACTGTATGTTGCTGTTACTCGTGCCAAGAAGCGGCTCATAATGACCAAATCCCTGGAGAACATCCTGACTCTGGCTGGG
Peptide sequence:
MEFTVCCCYSCQEAAHNDQIPGEHPDSGWAIMNIVLSQPCGKIFVGDPHQQIYTFRGAVNALFTVPHTHVFYLTQSFRFGVEIAYVGATILDVCKRVRKKTLVGGNHQSGIRGDIKGQVALLSRTNANVFDEAVRVTEGESPARIHLIGGIKSFGLDRIIDIWTLLQPEEERRKRDLIIKDRFIRRWVHKEGFSGFKRYVTAAEDKELEAKIAVVEKYNIRIPELVERIERCHIDDLDFAEYILGTVHKAKGLEFDTVHVLDDFVKVPCARHNLAQLPHFRVESFSEDEWNLLYVAVTRAKKRLIMTKSLENILTLAG
IRESfinder result
IRESfinder score
0.57431989
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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