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mmu_circFbh1_746
Basic information of circular RNA
PanCircBase ID:
mmu_circFbh1_746
mm39_Chr. Position ID:
chr2|11771932|11774742|-
Gene name:
Fbh1
Transcript:
ENSMUST00000071564.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
3,2
Exon/intron offsets:
0,2654
Genomic length:
2810
Exon/intron sizes in nt:
5,90,156
Flanking exon/introns:
chr2:11769044-11771932|chr2:11774742-11782242
Splice length:
746
Other database IDs:
CIRCpedia: MMU_CIRCpedia_214523, circAtlas: mmu-Fbxo18_0003
Human Ortholog IDs:
Mature spliced Sequence:
TGAGACGGTTTAAGCGGAAGCATCTTACAGTGGTCGACTGCCATCATTTGGCCCGGAGTCATTTGGCTGTGACTCAGCCCTTCAGTCAGAGATGGACAAACAGAGACCCTAACCATGGTCTCTATCCTAGACCCAGAACAAAAGGACGGAATAGGGGTCGAGGATGTCAGAGATATATTTCTGAGTTCTTCCTAGCTGGCCACCAGCACTGTACCAATGACATGGCCAAAAGCAATTCTGTTGGCCAGGACAGCTGTCAGGACGCTGAGGGTGACATGATTTTAACTGCAGAGAGCAGTTGTACTCTGCCTCAGGTGGATAATGGAGAAGCGAGGCTGGGCTCATCAGGATCTGCACAGCCTGCCAGGAAGCGGGCTCACTGCTTTGAGGAAGCCACAGAGAGCGGCCAGTGGGATGGCGTTACTAAGAAGACACCGCGCCATCGTTTGTTCCCATCGTGTTCAAGGCTTAGGGAGGCCAGGCAAGGGGCAGAGGACAGTTTGTCCCAGTGCTCCCCAGTGCCGGGGGAAGCTGGCCGAGACATTGAGGACATTGGTCCTGATCCCTTGCCTGACTCATACTATGGGCTTCTTGGAATGTTGCCTTGTCAGGAAGTGCCCAGCCACATTTGCAGGCTGCCAAGTGAAGTGCTGAGACACATCTTTGCTTTCCTGCCAGTGGAGGACCTCTACTGGAATCTCAGCCTGGTGTGCCACCTGTGGAGGGAAATAATCAATGACCCGCTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTTGCTTTCCTGCCAGTGGA
60.11
TCACAGCCAAATGACTCCGG
60.32
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTTGCTTTCCTGCCAGTGGA
60.11
AATGACTCCGGGCCAAATGA
59.67
147
PCR template sequence:
AGTGCTGAGACACATCTTTGCTTTCCTGCCAGTGGAGGACCTCTACTGGAATCTCAGCCTGGTGTGCCACCTGTGGAGGGAAATAATCAATGACCCGCTGTGAGACGGTTTAAGCGGAAGCATCTTACAGTGGTCGACTGCCATCATTTGGCCCGGAGTCATTTGGCTGTGACTCAGCCCTTCAGTCAGAGATGGACAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-709(1), mmu-miR-495-3p(1), mmu-miR-676-3p(1), mmu-miR-1199-5p(1), mmu-miR-697(1)
siRNAs for circular RNA silencing
Junction sequence
CAATGACCCGCTGTGAGACGGTTTAA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFBH1_746_ORF_1
0.6922
0.165005573
0.621069476
528
ORF sequence:
ATGGCCAAAAGCAATTCTGTTGGCCAGGACAGCTGTCAGGACGCTGAGGGTGACATGATTTTAACTGCAGAGAGCAGTTGTACTCTGCCTCAGGTGGATAATGGAGAAGCGAGGCTGGGCTCATCAGGATCTGCACAGCCTGCCAGGAAGCGGGCTCACTGCTTTGAGGAAGCCACAGAGAGCGGCCAGTGGGATGGCGTTACTAAGAAGACACCGCGCCATCGTTTGTTCCCATCGTGTTCAAGGCTTAGGGAGGCCAGGCAAGGGGCAGAGGACAGTTTGTCCCAGTGCTCCCCAGTGCCGGGGGAAGCTGGCCGAGACATTGAGGACATTGGTCCTGATCCCTTGCCTGACTCATACTATGGGCTTCTTGGAATGTTGCCTTGTCAGGAAGTGCCCAGCCACATTTGCAGGCTGCCAAGTGAAGTGCTGAGACACATCTTTGCTTTCCTGCCAGTGGAGGACCTCTACTGGAATCTCAGCCTGGTGTGCCACCTGTGGAGGGAAATAATCAATGACCCGCTGTGA
Peptide sequence:
MAKSNSVGQDSCQDAEGDMILTAESSCTLPQVDNGEARLGSSGSAQPARKRAHCFEEATESGQWDGVTKKTPRHRLFPSCSRLREARQGAEDSLSQCSPVPGEAGRDIEDIGPDPLPDSYYGLLGMLPCQEVPSHICRLPSEVLRHIFAFLPVEDLYWNLSLVCHLWREIINDPL
IRESfinder result
IRESfinder score
0.459491526
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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