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mmu_circFam161b_414
Basic information of circular RNA
PanCircBase ID:
mmu_circFam161b_414
mm39_Chr. Position ID:
chr12|84393560|84395544|-
Gene name:
Fam161b
Transcript:
ENSMUST00000065536.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
8,7,6
Exon/intron offsets:
0,1490,1819
Genomic length:
1984
Exon/intron sizes in nt:
149,100,165
Flanking exon/introns:
chr12:84393183-84393560|chr12:84395544-84400573
Splice length:
414
Other database IDs:
CIRCpedia: MMU_CIRCpedia_31637, circAtlas: mmu-Fam161b_0001
Human Ortholog IDs:
Mature spliced Sequence:
AATGTCACTTGAAAAGAAGGACAAATCAGACATGAGCATTCAGTGGTTGGAGGTGCACAAAAAGAACTGTCAAGCCATGTCTAAATCAGTGACCTTGAGGGCCAAGGCCATGGATCCCCACAAAAGCCTGGAAGAGGTGTTCAAAGCGAAGCTTAAAGAGAACCGGAGCAATGACCGTAAAAGAGCAAAGGAATATAAGAAAGAACTGGAGGAAATGAAGAAAAGAATACAAACAAGGCCCTATCTCTTTGAACAAGTTACCAAGGCCCTCGCCAGGAAAGAAGCAGAAGAGCGCTATAGGGACGCCCTGAAGCAGGCCGGCCTGGAGGAGGAGTTTGTGAGAACCAAGAGTCAAGGCACAGAGGCAGTGTGATGAAGAGAAAGCCAAAGTCAAGGAATCTCACCGGTTAAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCCTGGAGGAGGAGTTTGTG
60.32
TTTGTGCACCTCCAACCACT
60.03
155
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCCTGGAGGAGGAGTTTGTG
60.32
GTGCACCTCCAACCACTGAA
60.47
152
PCR template sequence:
AGGCCGGCCTGGAGGAGGAGTTTGTGAGAACCAAGAGTCAAGGCACAGAGGCAGTGTGATGAAGAGAAAGCCAAAGTCAAGGAATCTCACCGGTTAAGAGAATGTCACTTGAAAAGAAGGACAAATCAGACATGAGCATTCAGTGGTTGGAGGTGCACAAAAAGAACTGTCAAGCCATGTCTAAATCAGTGACCTTGAGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-135a-5p(1), mmu-miR-149-5p(1), mmu-miR-324-3p(1), mmu-miR-5101(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
CACCGGTTAAGAGAATGTCACTTGAA
siRNA sets
GTGACATTCTCTTAACCGG, AGTGACATTCTCTTAACCG, TGACATTCTCTTAACCGGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.262715389
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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