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mmu_circFam114a2_263
Basic information of circular RNA
PanCircBase ID:
mmu_circFam114a2_263
mm39_Chr. Position ID:
chr11|57382745|57383952|-
Gene name:
Fam114a2
Transcript:
ENSMUST00000020831.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
11,10
Exon/intron offsets:
0,1084
Genomic length:
1207
Exon/intron sizes in nt:
1,40,123
Flanking exon/introns:
chr11:57381008-57382745|chr11:57383952-57390551
Splice length:
263
Other database IDs:
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_164679
Human Ortholog IDs:
Mature spliced Sequence:
GCAAGAAATACAGCCTACAAATGGATCAGAACATCTCTGGCTAGGCCAGTAGCAGAGAAAGAAGAAGGAGAAAAAGAGTCGGAGGCAGGAAATACCGAGGAAGCCCAGAAACATTCAATAGAGGATATCCATGCATTTGCAATCCGGAGCTTAGCAGAGTTGACTGCCTGCTCAATAGAATTGTTCCACAAAACAGCAGCATTGGTTTTGCATGGCCAGAAGCAGGAGGTGACAGCCTTAGAGAGGAGCCAGACTCTCTCCCA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTTTTGCATGGCCAGAAGCA
59.97
CCTCGGTATTTCCTGCCTCC
59.89
159
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCAGAAGCAGGAGGTGACAG
60.04
CCTCGGTATTTCCTGCCTCC
59.89
148
PCR template sequence:
CTGCCTGCTCAATAGAATTGTTCCACAAAACAGCAGCATTGGTTTTGCATGGCCAGAAGCAGGAGGTGACAGCCTTAGAGAGGAGCCAGACTCTCTCCCAGCAAGAAATACAGCCTACAAATGGATCAGAACATCTCTGGCTAGGCCAGTAGCAGAGAAAGAAGAAGGAGAAAAAGAGTCGGAGGCAGGAAATACCGAGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-149-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AGACTCTCTCCCAGCAAGAAATACAG
siRNA sets
GTATTTCTTGCTGGGAGAG, CTGTATTTCTTGCTGGGAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.523370527
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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