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mmu_circFam114a1_369
Basic information of circular RNA
PanCircBase ID:
mmu_circFam114a1_369
mm39_Chr. Position ID:
chr5|65172658|65185844|+
Gene name:
Fam114a1
Transcript:
ENSMUST00000031080.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
7,8,9
Exon/intron offsets:
0,6286,13094
Genomic length:
13186
Exon/intron sizes in nt:
153,124,92
Flanking exon/introns:
chr5:65166465-65172658|chr5:65185844-65187407
Splice length:
369
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
ATGTTGAGAGAAGCCAAGGAGAAGGAGAAGCAGAGGTTGGCCCAGCAGCTCACAGTGGAGAGGACTGCACACTATGGGATGCTGTTTGATGAATATCAAGGTTTGTCACACCTGGAAGCCCTGGAAATTCTGTCCAATGAGAGTGAGAGCAAGGTTCAGTCCTTCTTAACATCCCTGGATGGAGAGAAGCTAGAACTCTTGAAAAATGACCTAATTTCAATCAAGGACATCTTTGTAGCCAAAGAATCAGAGAATGAAGCAAACCCAGAAGAACAAGGCCTAGAAGAAAGCGGGGAAGAATTTGCCAGGATGCTTACAGAGCTCCTGTTTGAATTACACGTCGCCGCCACACCTGACAAACTCAATAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCCTAGAAGAAAGCGGGGAA
59.75
AGTCCTCTCCACTGTGAGCT
59.89
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAGGCCTAGAAGAAAGCGGG
59.75
AGTCCTCTCCACTGTGAGCT
59.89
161
PCR template sequence:
AGAACAAGGCCTAGAAGAAAGCGGGGAAGAATTTGCCAGGATGCTTACAGAGCTCCTGTTTGAATTACACGTCGCCGCCACACCTGACAAACTCAATAAGATGTTGAGAGAAGCCAAGGAGAAGGAGAAGCAGAGGTTGGCCCAGCAGCTCACAGTGGAGAGGACTGCACACTATGGGATGCTGTTTGATGAATATCAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-324-5p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
CAAACTCAATAAGATGTTGAGAGAAG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCFAM114A1_369_ORF_1
1.0855
0.32293914
0.967838108
738
ORF sequence:
ATGTTGAGAGAAGCCAAGGAGAAGGAGAAGCAGAGGTTGGCCCAGCAGCTCACAGTGGAGAGGACTGCACACTATGGGATGCTGTTTGATGAATATCAAGGTTTGTCACACCTGGAAGCCCTGGAAATTCTGTCCAATGAGAGTGAGAGCAAGGTTCAGTCCTTCTTAACATCCCTGGATGGAGAGAAGCTAGAACTCTTGAAAAATGACCTAATTTCAATCAAGGACATCTTTGTAGCCAAAGAATCAGAGAATGAAGCAAACCCAGAAGAACAAGGCCTAGAAGAAAGCGGGGAAGAATTTGCCAGGATGCTTACAGAGCTCCTGTTTGAATTACACGTCGCCGCCACACCTGACAAACTCAATAAGATGTTGAGAGAAGCCAAGGAGAAGGAGAAGCAGAGGTTGGCCCAGCAGCTCACAGTGGAGAGGACTGCACACTATGGGATGCTGTTTGATGAATATCAAGGTTTGTCACACCTGGAAGCCCTGGAAATTCTGTCCAATGAGAGTGAGAGCAAGGTTCAGTCCTTCTTAACATCCCTGGATGGAGAGAAGCTAGAACTCTTGAAAAATGACCTAATTTCAATCAAGGACATCTTTGTAGCCAAAGAATCAGAGAATGAAGCAAACCCAGAAGAACAAGGCCTAGAAGAAAGCGGGGAAGAATTTGCCAGGATGCTTACAGAGCTCCTGTTTGAATTACACGTCGCCGCCACACCTGACAAACTCAATAAG
Peptide sequence:
MLREAKEKEKQRLAQQLTVERTAHYGMLFDEYQGLSHLEALEILSNESESKVQSFLTSLDGEKLELLKNDLISIKDIFVAKESENEANPEEQGLEESGEEFARMLTELLFELHVAATPDKLNKMLREAKEKEKQRLAQQLTVERTAHYGMLFDEYQGLSHLEALEILSNESESKVQSFLTSLDGEKLELLKNDLISIKDIFVAKESENEANPEEQGLEESGEEFARMLTELLFELHVAATPDKLNK
IRESfinder result
IRESfinder score
0.560161513
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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