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mmu_circEzh1_496
Basic information of circular RNA
PanCircBase ID:
mmu_circEzh1_496
mm39_Chr. Position ID:
chr11|101104549|101108194|-
Gene name:
Ezh1
Transcript:
ENSMUST00000100417.3
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
6,5,4,3
Exon/intron offsets:
0,1459,3271,3519
Genomic length:
3645
Exon/intron sizes in nt:
1,21,12,01,29,126
Flanking exon/introns:
chr11:101101549-101104549|chr11:101108194-101109479
Splice length:
496
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TCCAGGAAAATGGATATAGCAAGTCCCCCAACTTCCAAATGCATCACATACTGGAAAAGAAAAGTGAAATCTGAGTATATGCGGCTTCGACAGCTCAAACGGCTCCAGGCAAATATGGGAGCAAAGGCTCTGTATGTGGCAAATTTTGCAAAGGTTCAAGAAAAAACCCAAATCCTCAATGAAGAGTGGAAGAAACTTCGTGTCCAGCCTGTTCAGCCAATGAAGCCCGTGAGTGGGCACCCTTTTCTGAAAAAGTGTACCATAGAGAGCATTTTCCCAGGGTTCGACAGCCAGGATATGTTGATGCGGTCTCTGAACACTGTTGCACTGGTTCCCATCATGTATTCCTGGTCCCCTCTCCAGCAGAATTTCATGGTGGAAGATGAGACGGTTTTGTGCAATATTCCATACATGGGTGACGAGGTGAAGGAAGAAGATGAGACTTTCATCGAAGAGCTGATCAATAACTATGATGGCAAAGTCCACGGTGAAGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGGTGACGAGGTGAAGGAAG
60.04
TGTCGAAGCCGCATATACTCA
59.32
175
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACATGGGTGACGAGGTGAAG
59.68
TGTCGAAGCCGCATATACTCA
59.32
179
PCR template sequence:
TGCAATATTCCATACATGGGTGACGAGGTGAAGGAAGAAGATGAGACTTTCATCGAAGAGCTGATCAATAACTATGATGGCAAAGTCCACGGTGAAGAAGTCCAGGAAAATGGATATAGCAAGTCCCCCAACTTCCAAATGCATCACATACTGGAAAAGAAAAGTGAAATCTGAGTATATGCGGCTTCGACAGCTCAAAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-32-5p(1), mmu-miR-130a-3p(1), mmu-miR-25-3p(1), mmu-miR-363-3p(1), mmu-miR-130b-3p(1), mmu-miR-301b-3p(1), mmu-miR-297a-5p(1), mmu-miR-301a-3p(1), mmu-miR-325-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CACGGTGAAGAAGTCCAGGAAAATGG
siRNA sets
TTTTCCTGGACTTCTTCAC, TTTCCTGGACTTCTTCACC, ATTTTCCTGGACTTCTTCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEZH1_496_ORF_1
1.2027
0.262571811
0.890143305
561
ORF sequence:
ATGGATATAGCAAGTCCCCCAACTTCCAAATGCATCACATACTGGAAAAGAAAAGTGAAATCTGAGTATATGCGGCTTCGACAGCTCAAACGGCTCCAGGCAAATATGGGAGCAAAGGCTCTGTATGTGGCAAATTTTGCAAAGGTTCAAGAAAAAACCCAAATCCTCAATGAAGAGTGGAAGAAACTTCGTGTCCAGCCTGTTCAGCCAATGAAGCCCGTGAGTGGGCACCCTTTTCTGAAAAAGTGTACCATAGAGAGCATTTTCCCAGGGTTCGACAGCCAGGATATGTTGATGCGGTCTCTGAACACTGTTGCACTGGTTCCCATCATGTATTCCTGGTCCCCTCTCCAGCAGAATTTCATGGTGGAAGATGAGACGGTTTTGTGCAATATTCCATACATGGGTGACGAGGTGAAGGAAGAAGATGAGACTTTCATCGAAGAGCTGATCAATAACTATGATGGCAAAGTCCACGGTGAAGAAGTCCAGGAAAATGGATATAGCAAGTCCCCCAACTTCCAAATGCATCACATACTGGAAAAGAAAAGTGAAATCTGA
Peptide sequence:
MDIASPPTSKCITYWKRKVKSEYMRLRQLKRLQANMGAKALYVANFAKVQEKTQILNEEWKKLRVQPVQPMKPVSGHPFLKKCTIESIFPGFDSQDMLMRSLNTVALVPIMYSWSPLQQNFMVEDETVLCNIPYMGDEVKEEDETFIEELINNYDGKVHGEEVQENGYSKSPNFQMHHILEKKSEI
IRESfinder result
IRESfinder score
0.387468967
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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