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mmu_circEzh1_181
Basic information of circular RNA
PanCircBase ID:
mmu_circEzh1_181
mm39_Chr. Position ID:
chr11|101094586|101094767|-
Gene name:
Ezh1
Transcript:
ENSMUST00000107284.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
11
Exon/intron offsets:
0
Genomic length:
181
Exon/intron sizes in nt:
181
Flanking exon/introns:
chr11:101094277-101094586|chr11:101094767-101098163
Splice length:
181
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GAAGGAGCAAAGGAGTACGCCATGCTGCACAACCCTCGGTCCAAGTGCTCTGGGCGCCGCCGCCGAAGGCACCCAGTGGTCAGTGCTTCCTGCTCCAATGCATCAGCTTCTGCTATGGCTGAAACTAAAGAAGGAGACAGTGATAGAGACACTGGCAATGACTGGGCCTCCAGTTCTTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGACACTGGCAATGACTGGG
59.96
GGAAGCACTGACCACTGGG
60.3
125
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCCAATGCATCAGCTTCTGC
59.93
CACTTGGACCGAGGGTTGTG
60.88
136
PCR template sequence:
GCTCCAATGCATCAGCTTCTGCTATGGCTGAAACTAAAGAAGGAGACAGTGATAGAGACACTGGCAATGACTGGGCCTCCAGTTCTTCAGGAAGGAGCAAAGGAGTACGCCATGCTGCACAACCCTCGGTCCAAGTGCTCTGGGCGCCGCCGCCGAAGGCACCCAGTGGTCAGTGCTTCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-199a-5p(1), mmu-miR-33-5p(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TCCAGTTCTTCAGGAAGGAGCAAAGG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEZH1_181_ORF_1
0.7705
0.127540854
0.220581151
282
ORF sequence:
ATGCTGCACAACCCTCGGTCCAAGTGCTCTGGGCGCCGCCGCCGAAGGCACCCAGTGGTCAGTGCTTCCTGCTCCAATGCATCAGCTTCTGCTATGGCTGAAACTAAAGAAGGAGACAGTGATAGAGACACTGGCAATGACTGGGCCTCCAGTTCTTCAGGAAGGAGCAAAGGAGTACGCCATGCTGCACAACCCTCGGTCCAAGTGCTCTGGGCGCCGCCGCCGAAGGCACCCAGTGGTCAGTGCTTCCTGCTCCAATGCATCAGCTTCTGCTATGGCTGA
Peptide sequence:
MLHNPRSKCSGRRRRRHPVVSASCSNASASAMAETKEGDSDRDTGNDWASSSSGRSKGVRHAAQPSVQVLWAPPPKAPSGQCFLLQCISFCYG
IRESfinder result
IRESfinder score
0.173547892
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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