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mmu_circExoc1_809
Basic information of circular RNA
PanCircBase ID:
mmu_circExoc1_809
mm39_Chr. Position ID:
chr5|76689897|76697448|+
Gene name:
Exoc1
Transcript:
ENSMUST00000087133.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
5,6,7,8,9
Exon/intron offsets:
0,1404,1827,3203,7401
Genomic length:
7551
Exon/intron sizes in nt:
18,82,28,13,31,10,150
Flanking exon/introns:
chr5:76685704-76689897|chr5:76697448-76701946
Splice length:
809
Other database IDs:
circAtlas: mmu-Exoc1_0005
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_363853
Human Ortholog IDs:
Mature spliced Sequence:
AATCTGTTCCAAGCGGAGAGAATCAGAGTGTAGCGGGAGGGGATGAAGAAGCGGTAGATGAGTACCAAGAGTTAAATGCAAGAGAAGAGCAGGACATCGAGATAATGATGGAAGGCTGCGAGTGCGCCATTTCTAACGCTGAAGCCTTTGCGGAGAAGTTGTCCCGGGAGCTGCAGGTGCTCGATGGGGCCAACATTCAGTCAATCATGGCCTCTGAGAAACAAGTGAACACCCTGATGCAGTTGCTGGATGAGGCGCTAACGGAGGTGGACCAGATTGAACTGAAACTGAGCAGTTACGAGGAGATGCTCCAAAGTGTGAAAGAGCAGATGGATCAGATCTCTGAGAGCAACCACCTGATCCATCTCAGCAACACGAATAATGTGAAGCTCCTGTCCGAGATAGAGTTCCTCGTGAACCACATGGACTTGGCCAAAGGTCATATAAAGGCCCTTCAGGAAGGAGATCTTGTTTCTTCCCGAGGCATCGAGGCCTGCACCAACGCTGCTGACGCCCTGCTGCAGTGCATGAACGTTGCTCTTCGCCCAGGCCATGACATGCTCCTGGCCGTCAAGCAGCAGCAGCAGCGCTTCAGTGACCTGAGGGAACATTTCGCCCGGAGACTGGCCAGCCATCTCAACAATGTTTTTGTTCAACAGGGTCATGATCAGAGTTCAACTCTTGCTCAGCACTCTGTTGAACTGACTCTACCCAATCACCATCCATTTCATAGAGACTTGCTCCGCTATGCCAAGCTGATGGAGTGGCTAAAGAGTACAGACTATGGAAAATACGAGGGACTAACAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGAGACTTGCTCCGCTATGC
59.9
TGTCCTGCTCTTCTCTTGCA
58.95
174
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCCGCTATGCCAAGCTGAT
60.25
TGTCCTGCTCTTCTCTTGCA
58.95
165
PCR template sequence:
ACCCAATCACCATCCATTTCATAGAGACTTGCTCCGCTATGCCAAGCTGATGGAGTGGCTAAAGAGTACAGACTATGGAAAATACGAGGGACTAACAAAGAATCTGTTCCAAGCGGAGAGAATCAGAGTGTAGCGGGAGGGGATGAAGAAGCGGTAGATGAGTACCAAGAGTTAAATGCAAGAGAAGAGCAGGACATCGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-223-3p(1), mmu-miR-217-5p(1), mmu-miR-10b-5p(1), mmu-miR-10a-5p(1), mmu-miR-503-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGGACTAACAAAGAATCTGTTCCAAG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEXOC1_809_ORF_1
1.2829
0.376305445
0.98145838
738
ORF sequence:
ATGATGGAAGGCTGCGAGTGCGCCATTTCTAACGCTGAAGCCTTTGCGGAGAAGTTGTCCCGGGAGCTGCAGGTGCTCGATGGGGCCAACATTCAGTCAATCATGGCCTCTGAGAAACAAGTGAACACCCTGATGCAGTTGCTGGATGAGGCGCTAACGGAGGTGGACCAGATTGAACTGAAACTGAGCAGTTACGAGGAGATGCTCCAAAGTGTGAAAGAGCAGATGGATCAGATCTCTGAGAGCAACCACCTGATCCATCTCAGCAACACGAATAATGTGAAGCTCCTGTCCGAGATAGAGTTCCTCGTGAACCACATGGACTTGGCCAAAGGTCATATAAAGGCCCTTCAGGAAGGAGATCTTGTTTCTTCCCGAGGCATCGAGGCCTGCACCAACGCTGCTGACGCCCTGCTGCAGTGCATGAACGTTGCTCTTCGCCCAGGCCATGACATGCTCCTGGCCGTCAAGCAGCAGCAGCAGCGCTTCAGTGACCTGAGGGAACATTTCGCCCGGAGACTGGCCAGCCATCTCAACAATGTTTTTGTTCAACAGGGTCATGATCAGAGTTCAACTCTTGCTCAGCACTCTGTTGAACTGACTCTACCCAATCACCATCCATTTCATAGAGACTTGCTCCGCTATGCCAAGCTGATGGAGTGGCTAAAGAGTACAGACTATGGAAAATACGAGGGACTAACAAAGAATCTGTTCCAAGCGGAGAGAATCAGAGTGTAG
Peptide sequence:
MMEGCECAISNAEAFAEKLSRELQVLDGANIQSIMASEKQVNTLMQLLDEALTEVDQIELKLSSYEEMLQSVKEQMDQISESNHLIHLSNTNNVKLLSEIEFLVNHMDLAKGHIKALQEGDLVSSRGIEACTNAADALLQCMNVALRPGHDMLLAVKQQQQRFSDLREHFARRLASHLNNVFVQQGHDQSSTLAQHSVELTLPNHHPFHRDLLRYAKLMEWLKSTDYGKYEGLTKNLFQAERIRV
IRESfinder result
IRESfinder score
0.353465778
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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