top of page
mmu_circEvi5_550
Basic information of circular RNA
PanCircBase ID:
mmu_circEvi5_550
mm39_Chr. Position ID:
chr5|107943564|107961472|-
Gene name:
Evi5
Transcript:
ENSMUST00000112642.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
14,13,12,11,10
Exon/intron offsets:
0,2114,3427,16659,17847
Genomic length:
17908
Exon/intron sizes in nt:
159,141,135,54,61
Flanking exon/introns:
chr5:107938374-107943564|chr5:107961472-107963476
Splice length:
550
Other database IDs:
circAtlas: mmu-Evi5_0017
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
ACTTGAAAAAGAATACACTACAATAAAAACTAAAGAAATGGAAGAACAAGGTGAAATTAAAAGGCTACGCACAGAAAATAGGCTTTTAAAACAGCGCATTGAAACATTAGAAAAACATAAATGCAGTTCCACCTACAACGAAGATTTCGTGCTGCAGCTGGAGAAGGAATTGGTTCAAGCCAGACTGAGTGAAGCCGAGTCTCAGTGTGCACTGAAGGAGATGCAGGATAAAGTGTTGGATATAGAGAAGAAGAACAACTCATTTCCTGATGAGAATAATATTGCAAGGCTTCAGGAGGAACTCATCGCTGTGAAGCTGAGGGAAGCCGAAGCCATTATGGGTTTGAAAGAACTTCGACAGCAAGTCAGAACCTTAGAAGAGCACTGGCAGCGTCACTTAGCTCGAACTTCTGGAAGATGGAAAGACCCACCCAAGAAAAATGCTGTGAATGAGTTACAGGATGAGCTGATGAGCATTCGCCTTAGAGAGGCGGAAACACAGGCAGAAATAAGAGAGATGAAGCAAAGGATGATGGAAATGGAGACACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCGGAAACACAGGCAGAAAT
59.4
ATGCGCTGTTTTAAAAGCCT
56.89
159
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCGGAAACACAGGCAGAAAT
59.4
AATGCGCTGTTTTAAAAGCCT
57.61
160
PCR template sequence:
TGAGTTACAGGATGAGCTGATGAGCATTCGCCTTAGAGAGGCGGAAACACAGGCAGAAATAAGAGAGATGAAGCAAAGGATGATGGAAATGGAGACACAGACTTGAAAAAGAATACACTACAATAAAAACTAAAGAAATGGAAGAACAAGGTGAAATTAAAAGGCTACGCACAGAAAATAGGCTTTTAAAACAGCGCATT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(1), mmu-miR-27a-3p(1), mmu-miR-135a-5p(1), mmu-miR-211-5p(1), mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-673-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AATGGAGACACAGACTTGAAAAAGAA
siRNA sets
TTTCAAGTCTGTGTCTCCA, CTTTTTCAAGTCTGTGTCT, TTCTTTTTCAAGTCTGTGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEVI5_550_ORF_1
1.1051
0.256240901
0.829091541
519
ORF sequence:
ATGGAAGAACAAGGTGAAATTAAAAGGCTACGCACAGAAAATAGGCTTTTAAAACAGCGCATTGAAACATTAGAAAAACATAAATGCAGTTCCACCTACAACGAAGATTTCGTGCTGCAGCTGGAGAAGGAATTGGTTCAAGCCAGACTGAGTGAAGCCGAGTCTCAGTGTGCACTGAAGGAGATGCAGGATAAAGTGTTGGATATAGAGAAGAAGAACAACTCATTTCCTGATGAGAATAATATTGCAAGGCTTCAGGAGGAACTCATCGCTGTGAAGCTGAGGGAAGCCGAAGCCATTATGGGTTTGAAAGAACTTCGACAGCAAGTCAGAACCTTAGAAGAGCACTGGCAGCGTCACTTAGCTCGAACTTCTGGAAGATGGAAAGACCCACCCAAGAAAAATGCTGTGAATGAGTTACAGGATGAGCTGATGAGCATTCGCCTTAGAGAGGCGGAAACACAGGCAGAAATAAGAGAGATGAAGCAAAGGATGATGGAAATGGAGACACAGACTTGA
Peptide sequence:
MEEQGEIKRLRTENRLLKQRIETLEKHKCSSTYNEDFVLQLEKELVQARLSEAESQCALKEMQDKVLDIEKKNNSFPDENNIARLQEELIAVKLREAEAIMGLKELRQQVRTLEEHWQRHLARTSGRWKDPPKKNAVNELQDELMSIRLREAETQAEIREMKQRMMEMETQT
IRESfinder result
IRESfinder score
0.355939382
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page