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mmu_circEvi5_1339
Basic information of circular RNA
PanCircBase ID:
mmu_circEvi5_1339
mm39_Chr. Position ID:
chr5|107945678|107969760|-
Gene name:
Evi5
Transcript:
ENSMUST00000112642.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
11
Exon/intron indexes:
13,12,11,10,9,8,7,6,5,4,3
Exon/intron offsets:
0,1313,14545,15733,17798,17989,18961,21076,21791,22619,23892
Genomic length:
24082
Exon/intron sizes in nt:
1,41,13,55,46,19,89,01,00,00,00,00,00,000
Flanking exon/introns:
chr5:107943723-107945678|chr5:107969760-107989936
Splice length:
1339
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
ATTAATAGAGACAGACAGTAAGTCTTTAAGATCAGTGAATGGGTCAAGAAGAAACAGTGGTTCCTCTCTTGTATCAAGTTCATCAGCCTCCAGCAACCTCAGCCACCTGGAGGAAGACTCTTGGATTCTCTGGGGAAGAATTGTGAATGAATGGGATGATGTACGCAAGAAGAAAGAAAAGCAAGTGAAGGAGCTTGTTCGTAAAGGGATACCCCATCACTTCAGAGCAATAGTGTGGCAACTTTTATGTAATGCACAGAGTATGACAATTAAGGACCAGTATTCAGAACTCCTGAAAATGACTTCACCTTGTGAAAAACTGATACGAAGGGACATTGCTAGAACTTATCCAGAACACAACTTTTTTAAGGAAAAAGATAGCCTTGGACAGGAGGTTTTATTTAATGTAATGAAGGCATACTCCTTAGTGGACCGTGAGGTTGGTTACTGTCAGGGCAGTGCTTTCATCGTTGGATTGTTGCTGATGCAGATGCCAGAAGAAGAGGCTTTTTGTGTATTTGTTAAATTAATGCAAGACTACAGACTTCGTGAACTATTTAAGCCAAGTATGGCGGAATTGGGCCTTTGTATGTACCAGTTTGAATGTATGATACAGGAGTATCTTCCGGAACTCTTTGTACATTTTCAATCTCAGAGTTTTCATACGTCAATGTATGCATCATCCTGGTTCCTGACAATCTTTCTTACAACTTTCCCACTACCAATTGCGACAAGGATATTTGACATCTTTATGTCTGAGGGTTTGGAAATAGTGTTTCGAGTAGGATTAGCGCTTCTTCAGATGAATCAGGCAGAATTAATGCAACTTGACATGGAAGGGATGTTACAGCACTTTCAGAAGGTCATTCCACATCAGTTTGATGGTGGCCCAGAAAAATTAATCCAGTCAGCTTACCAAGTCAAATACAACTCAAAAAAAATGAAAAAACTTGAAAAAGAATACACTACAATAAAAACTAAAGAAATGGAAGAACAAGGTGAAATTAAAAGGCTACGCACAGAAAATAGGCTTTTAAAACAGCGCATTGAAACATTAGAAAAACATAAATGCAGTTCCACCTACAACGAAGATTTCGTGCTGCAGCTGGAGAAGGAATTGGTTCAAGCCAGACTGAGTGAAGCCGAGTCTCAGTGTGCACTGAAGGAGATGCAGGATAAAGTGTTGGATATAGAGAAGAAGAACAACTCATTTCCTGATGAGAATAATATTGCAAGGCTTCAGGAGGAACTCATCGCTGTGAAGCTGAGGGAAGCCGAAGCCATTATGGGTTTGAAAGAACTTCGACAGCAAGTCAGAACCTTAGAAGAGCACTGGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAGGGAAGCCGAAGCCATTA
59.82
GGTTGCTGGAGGCTGATGAA
60.32
171
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAGGGAAGCCGAAGCCATTA
59.82
TGCTGGAGGCTGATGAACTT
59.31
168
PCR template sequence:
TCAGGAGGAACTCATCGCTGTGAAGCTGAGGGAAGCCGAAGCCATTATGGGTTTGAAAGAACTTCGACAGCAAGTCAGAACCTTAGAAGAGCACTGGCAGATTAATAGAGACAGACAGTAAGTCTTTAAGATCAGTGAATGGGTCAAGAAGAAACAGTGGTTCCTCTCTTGTATCAAGTTCATCAGCCTCCAGCAACCTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
22
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(1), mmu-miR-27a-3p(1), mmu-miR-106a-5p(1), mmu-miR-93-5p(1), mmu-miR-429-3p(1), mmu-miR-181a-5p(1), mmu-miR-135a-5p(1), mmu-miR-205-5p(1), mmu-miR-211-5p(1), mmu-miR-106b-5p(1), mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-381-3p(1), mmu-miR-382-3p(1), mmu-miR-382-5p(1), mmu-miR-680(1), mmu-miR-802-5p(1), mmu-miR-673-5p(1), mmu-miR-335-3p(1), mmu-miR-532-5p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AGAGCACTGGCAGATTAATAGAGACA
siRNA sets
TGTCTCTATTAATCTGCCA, TATTAATCTGCCAGTGCTC, CTATTAATCTGCCAGTGCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEVI5_1339_ORF_1
0.9003
0.075370984
0.990050431
1098
ORF sequence:
ATGACAATTAAGGACCAGTATTCAGAACTCCTGAAAATGACTTCACCTTGTGAAAAACTGATACGAAGGGACATTGCTAGAACTTATCCAGAACACAACTTTTTTAAGGAAAAAGATAGCCTTGGACAGGAGGTTTTATTTAATGTAATGAAGGCATACTCCTTAGTGGACCGTGAGGTTGGTTACTGTCAGGGCAGTGCTTTCATCGTTGGATTGTTGCTGATGCAGATGCCAGAAGAAGAGGCTTTTTGTGTATTTGTTAAATTAATGCAAGACTACAGACTTCGTGAACTATTTAAGCCAAGTATGGCGGAATTGGGCCTTTGTATGTACCAGTTTGAATGTATGATACAGGAGTATCTTCCGGAACTCTTTGTACATTTTCAATCTCAGAGTTTTCATACGTCAATGTATGCATCATCCTGGTTCCTGACAATCTTTCTTACAACTTTCCCACTACCAATTGCGACAAGGATATTTGACATCTTTATGTCTGAGGGTTTGGAAATAGTGTTTCGAGTAGGATTAGCGCTTCTTCAGATGAATCAGGCAGAATTAATGCAACTTGACATGGAAGGGATGTTACAGCACTTTCAGAAGGTCATTCCACATCAGTTTGATGGTGGCCCAGAAAAATTAATCCAGTCAGCTTACCAAGTCAAATACAACTCAAAAAAAATGAAAAAACTTGAAAAAGAATACACTACAATAAAAACTAAAGAAATGGAAGAACAAGGTGAAATTAAAAGGCTACGCACAGAAAATAGGCTTTTAAAACAGCGCATTGAAACATTAGAAAAACATAAATGCAGTTCCACCTACAACGAAGATTTCGTGCTGCAGCTGGAGAAGGAATTGGTTCAAGCCAGACTGAGTGAAGCCGAGTCTCAGTGTGCACTGAAGGAGATGCAGGATAAAGTGTTGGATATAGAGAAGAAGAACAACTCATTTCCTGATGAGAATAATATTGCAAGGCTTCAGGAGGAACTCATCGCTGTGAAGCTGAGGGAAGCCGAAGCCATTATGGGTTTGAAAGAACTTCGACAGCAAGTCAGAACCTTAGAAGAGCACTGGCAGATTAATAGAGACAGACAGTAA
Peptide sequence:
MTIKDQYSELLKMTSPCEKLIRRDIARTYPEHNFFKEKDSLGQEVLFNVMKAYSLVDREVGYCQGSAFIVGLLLMQMPEEEAFCVFVKLMQDYRLRELFKPSMAELGLCMYQFECMIQEYLPELFVHFQSQSFHTSMYASSWFLTIFLTTFPLPIATRIFDIFMSEGLEIVFRVGLALLQMNQAELMQLDMEGMLQHFQKVIPHQFDGGPEKLIQSAYQVKYNSKKMKKLEKEYTTIKTKEMEEQGEIKRLRTENRLLKQRIETLEKHKCSSTYNEDFVLQLEKELVQARLSEAESQCALKEMQDKVLDIEKKNNSFPDENNIARLQEELIAVKLREAEAIMGLKELRQQVRTLEEHWQINRDRQ
IRESfinder result
IRESfinder score
0.638411622
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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