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mmu_circEvi5_115

Basic information of circular RNA
PanCircBase ID:

mmu_circEvi5_115

mm39_Chr. Position ID:

chr5|107960223|107961472|-

Gene name:

Evi5

Transcript:

ENSMUST00000112642.8

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

2

Exon/intron indexes:

11,10

Exon/intron offsets:

0,1188

Genomic length:

1249

Exon/intron sizes in nt:

54,61

Flanking exon/introns:

chr5:107947126-107960223|chr5:107961472-107963476

Splice length:

115

Other database IDs:

circAtlas: hsa-SCAF8_0001

Human Ortholog IDs:
Mature spliced Sequence:

ACTTGAAAAAGAATACACTACAATAAAAACTAAAGAAATGGAAGAACAAGGTGAAATTAAAAGGCTACGCACAGAAAATAGGCTTTTAAAACAGCGCATTGAAACATTAGAAAAA

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GGCTACGCACAGAAAATAGGC

59.67

TCACCTTGTTCTTCCATTTCT

55.03

107

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GGCTACGCACAGAAAATAGGC

59.67

TCACCTTGTTCTTCCATTTCTT

55.83

107

PCR template sequence:

AAAAGGCTACGCACAGAAAATAGGCTTTTAAAACAGCGCATTGAAACATTAGAAAAAACTTGAAAAAGAATACACTACAATAAAAACTAAAGAAATGGAAGAACAAGGTGAAAT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

NA

Interacting miRNAs (#binding sites)

NA

siRNAs for circular RNA silencing
Junction sequence

AACATTAGAAAAAACTTGAAAAAGAA

siRNA sets

TTTTTCAAGTTTTTTCTAA, TTTTCAAGTTTTTTCTAAT, TTCTTTTTCAAGTTTTTTC

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCEVI5_115_ORF_1

0.9172

-0.094867084

0.034739307

84

ORF sequence:

ATGGAAGAACAAGGTGAAATTAAAAGGCTACGCACAGAAAATAGGCTTTTAAAACAGCGCATTGAAACATTAGAAAAAACTTGA

Peptide sequence:

MEEQGEIKRLRTENRLLKQRIETLEKT

IRESfinder result

IRESfinder score

0.547136295

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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Institute of Life sciences, Bhubaneswar, India

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