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mmu_circEva1c_618
Basic information of circular RNA
PanCircBase ID:
mmu_circEva1c_618
mm39_Chr. Position ID:
chr16|90663142|90687561|+
Gene name:
Eva1c
Transcript:
ENSMUST00000232274.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
3,4,5,6
Exon/intron offsets:
0,3311,9784,24275
Genomic length:
24419
Exon/intron sizes in nt:
1,97,12,41,53,144
Flanking exon/introns:
chr16:90661377-90663142|chr16:90687561-90691465
Splice length:
618
Other database IDs:
circBase: hsa_circ_0061482, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_35244
Human Ortholog IDs:
Mature spliced Sequence:
GTTACTTAACCAAACTCCTGCAAAACCACACTGCCTATGCCTGTGACGGGGACTATCTGAATCTACAGTGCCCTCGGCATTCAACAATAAGTGTCCAGTCGGCATTTTATGGGCAAGATTACCAAATGTGTAGTTCCCAGGAGCCCATCTCCCAGAGGGAAGACAACCTGACCTGTGTGGCATCCACCACTTTGCAGAAGGTGCTGGACGAGTGCCAGAACCAGCGGGCCTGCCACCTCCTGGTCAATAGCCGAGTCTTCGGACCTGACCTTTGTCCAGGAAGCAGTAAATACCTCCTGGTCTCCTTTAAATGCCAACCTAATGAGTTGAAAAACAAGACAGTGTGTGAAAACCAGGAGTTGAAGCTACACTGCCATGAGTCCAAGTTCCTCAACATCTACTCCGCCGCCTACGGCCGGAGGACCCAGCAAAGAGACATCTGCTCCTCCGGGGCGGAGCTGCTGCCCCCCTTCGACTGCCTGTCTTACACGGCTTTACAAGTCCTGTCCAGGAGGTGCTATGGGAAGCAGAGATGTAAGGTCCTCGTCGATAATTACCACTTCGGAAGCCCCTGTCTTCCAGGAGTGAAAAAATACCTCACTGTTGCCTACGCATGTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATTACCACTTCGGAAGCCCC
59.74
CCGAGGGCACTGTAGATTCA
59.18
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATTACCACTTCGGAAGCCCC
59.74
CCGAGGGCACTGTAGATTCAG
60.2
143
PCR template sequence:
TATGGGAAGCAGAGATGTAAGGTCCTCGTCGATAATTACCACTTCGGAAGCCCCTGTCTTCCAGGAGTGAAAAAATACCTCACTGTTGCCTACGCATGTGGTTACTTAACCAAACTCCTGCAAAACCACACTGCCTATGCCTGTGACGGGGACTATCTGAATCTACAGTGCCCTCGGCATTCAACAATAAGTGTCCAGTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(2), mmu-miR-34a-5p(2), mmu-miR-7b-5p(1), mmu-miR-106b-5p(1), mmu-miR-7a-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-511-3p(1), mmu-miR-34b-5p(1), mmu-miR-377-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GCCTACGCATGTGGTTACTTAACCAA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEVA1C_618_ORF_1
0.9005
0.233317459
0.995351967
1110
ORF sequence:
ATGTGTAGTTCCCAGGAGCCCATCTCCCAGAGGGAAGACAACCTGACCTGTGTGGCATCCACCACTTTGCAGAAGGTGCTGGACGAGTGCCAGAACCAGCGGGCCTGCCACCTCCTGGTCAATAGCCGAGTCTTCGGACCTGACCTTTGTCCAGGAAGCAGTAAATACCTCCTGGTCTCCTTTAAATGCCAACCTAATGAGTTGAAAAACAAGACAGTGTGTGAAAACCAGGAGTTGAAGCTACACTGCCATGAGTCCAAGTTCCTCAACATCTACTCCGCCGCCTACGGCCGGAGGACCCAGCAAAGAGACATCTGCTCCTCCGGGGCGGAGCTGCTGCCCCCCTTCGACTGCCTGTCTTACACGGCTTTACAAGTCCTGTCCAGGAGGTGCTATGGGAAGCAGAGATGTAAGGTCCTCGTCGATAATTACCACTTCGGAAGCCCCTGTCTTCCAGGAGTGAAAAAATACCTCACTGTTGCCTACGCATGTGGTTACTTAACCAAACTCCTGCAAAACCACACTGCCTATGCCTGTGACGGGGACTATCTGAATCTACAGTGCCCTCGGCATTCAACAATAAGTGTCCAGTCGGCATTTTATGGGCAAGATTACCAAATGTGTAGTTCCCAGGAGCCCATCTCCCAGAGGGAAGACAACCTGACCTGTGTGGCATCCACCACTTTGCAGAAGGTGCTGGACGAGTGCCAGAACCAGCGGGCCTGCCACCTCCTGGTCAATAGCCGAGTCTTCGGACCTGACCTTTGTCCAGGAAGCAGTAAATACCTCCTGGTCTCCTTTAAATGCCAACCTAATGAGTTGAAAAACAAGACAGTGTGTGAAAACCAGGAGTTGAAGCTACACTGCCATGAGTCCAAGTTCCTCAACATCTACTCCGCCGCCTACGGCCGGAGGACCCAGCAAAGAGACATCTGCTCCTCCGGGGCGGAGCTGCTGCCCCCCTTCGACTGCCTGTCTTACACGGCTTTACAAGTCCTGTCCAGGAGGTGCTATGGGAAGCAGAGATGTAAGGTCCTCGTCGATAATTACCACTTCGGAAGCCCCTGTCTTCCAGGAGTGAAAAAATACCTCACTGTTGCCTACGCATGT
Peptide sequence:
MCSSQEPISQREDNLTCVASTTLQKVLDECQNQRACHLLVNSRVFGPDLCPGSSKYLLVSFKCQPNELKNKTVCENQELKLHCHESKFLNIYSAAYGRRTQQRDICSSGAELLPPFDCLSYTALQVLSRRCYGKQRCKVLVDNYHFGSPCLPGVKKYLTVAYACGYLTKLLQNHTAYACDGDYLNLQCPRHSTISVQSAFYGQDYQMCSSQEPISQREDNLTCVASTTLQKVLDECQNQRACHLLVNSRVFGPDLCPGSSKYLLVSFKCQPNELKNKTVCENQELKLHCHESKFLNIYSAAYGRRTQQRDICSSGAELLPPFDCLSYTALQVLSRRCYGKQRCKVLVDNYHFGSPCLPGVKKYLTVAYAC
IRESfinder result
IRESfinder score
0.493539152
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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