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mmu_circEva1c_312
Basic information of circular RNA
PanCircBase ID:
mmu_circEva1c_312
mm39_Chr. Position ID:
chr16|90687417|90694341|+
Gene name:
Eva1c
Transcript:
ENSMUST00000232274.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
6,7,8
Exon/intron offsets:
0,4048,6834
Genomic length:
6924
Exon/intron sizes in nt:
144,78,90
Flanking exon/introns:
chr16:90673079-90687417|chr16:90694341-90700991
Splice length:
312
Other database IDs:
CIRCpedia: MMU_CIRCpedia_218506
Human Ortholog IDs:
Mature spliced Sequence:
ACTGCCTGTCTTACACGGCTTTACAAGTCCTGTCCAGGAGGTGCTATGGGAAGCAGAGATGTAAGGTCCTCGTCGATAATTACCACTTCGGAAGCCCCTGTCTTCCAGGAGTGAAAAAATACCTCACTGTTGCCTACGCATGTGTTCCCAAGAACATACTCACGGCAGTGGATCCGGCTGTTGCTAATCTAAATCCTTCTCTGAAGAAAGATGATGAACACGGTATAACATTCAACCCAAGCGGGTCGAGGGTTGTGCGGAAAGATGGCGTTATCGTCAGCAACTCTCTGGCCGCGTTCGCTTACATTCGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGCGTTATCGTCAGCAACT
60.04
GGGGCTTCCGAAGTGGTAAT
59.74
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCGGAAAGATGGCGTTATCG
59.77
GGGGCTTCCGAAGTGGTAAT
59.74
154
PCR template sequence:
GATGAACACGGTATAACATTCAACCCAAGCGGGTCGAGGGTTGTGCGGAAAGATGGCGTTATCGTCAGCAACTCTCTGGCCGCGTTCGCTTACATTCGAGACTGCCTGTCTTACACGGCTTTACAAGTCCTGTCCAGGAGGTGCTATGGGAAGCAGAGATGTAAGGTCCTCGTCGATAATTACCACTTCGGAAGCCCCTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-7b-5p(1), mmu-miR-7a-5p(1), mmu-miR-434-5p(1), mmu-miR-511-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GCTTACATTCGAGACTGCCTGTCTTA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEVA1C_312_ORF_1
0.757
-0.053808647
0.052108115
162
ORF sequence:
ATGGCGTTATCGTCAGCAACTCTCTGGCCGCGTTCGCTTACATTCGAGACTGCCTGTCTTACACGGCTTTACAAGTCCTGTCCAGGAGGTGCTATGGGAAGCAGAGATGTAAGGTCCTCGTCGATAATTACCACTTCGGAAGCCCCTGTCTTCCAGGAGTGA
Peptide sequence:
MALSSATLWPRSLTFETACLTRLYKSCPGGAMGSRDVRSSSIITTSEAPVFQE
IRESfinder result
IRESfinder score
0.322106387
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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