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mmu_circEts2_303
Basic information of circular RNA
PanCircBase ID:
mmu_circEts2_303
mm39_Chr. Position ID:
chr16|95515380|95516229|+
Gene name:
Ets2
Transcript:
ENSMUST00000023612.17
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
6,7
Exon/intron offsets:
0,630
Genomic length:
849
Exon/intron sizes in nt:
84,219
Flanking exon/introns:
chr16:95513330-95515380|chr16:95516229-95517077
Splice length:
303
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
AGAACCAAGAAAAGACAGAAGACCAATATGAGGAAAACTCTCACCTCAACGCGGTTCCTCATTGGATCAACAGCAATACATTAGGCTTCAGCATGGAACAGGCTCCATATGGAATGCAGGCACCAAACTACCCCAAAGACAATCTCCTGGACAGCATGTGCCCGCCATCGGCCACGCCTGCAGCTCTGGGCTCTGAGCTCCAGATGTTGCCCAAGTCTCGGCTCAACACCGTCAATGTCAATTACTGTTCCATCAGCCAGGACTTCCCCAGCAGCAACGTGAATTTGCTCAACAACAATTCTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGCTCAACACCGTCAATGTC
59.48
CAATGAGGAACCGCGTTGAG
59.56
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCTCAACACCGTCAATGTC
59.48
AATGAGGAACCGCGTTGAGG
60.67
147
PCR template sequence:
ATGTTGCCCAAGTCTCGGCTCAACACCGTCAATGTCAATTACTGTTCCATCAGCCAGGACTTCCCCAGCAGCAACGTGAATTTGCTCAACAACAATTCTGAGAACCAAGAAAAGACAGAAGACCAATATGAGGAAAACTCTCACCTCAACGCGGTTCCTCATTGGATCAACAGCAATACATTAGGCTTCAGCATGGAACA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-666-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AACAACAATTCTGAGAACCAAGAAAA
siRNA sets
TTGGTTCTCAGAATTGTTG, TCTTGGTTCTCAGAATTGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCETS2_303_ORF_1
1.0244
0.413023072
0.884767351
513
ORF sequence:
ATGGAACAGGCTCCATATGGAATGCAGGCACCAAACTACCCCAAAGACAATCTCCTGGACAGCATGTGCCCGCCATCGGCCACGCCTGCAGCTCTGGGCTCTGAGCTCCAGATGTTGCCCAAGTCTCGGCTCAACACCGTCAATGTCAATTACTGTTCCATCAGCCAGGACTTCCCCAGCAGCAACGTGAATTTGCTCAACAACAATTCTGAGAACCAAGAAAAGACAGAAGACCAATATGAGGAAAACTCTCACCTCAACGCGGTTCCTCATTGGATCAACAGCAATACATTAGGCTTCAGCATGGAACAGGCTCCATATGGAATGCAGGCACCAAACTACCCCAAAGACAATCTCCTGGACAGCATGTGCCCGCCATCGGCCACGCCTGCAGCTCTGGGCTCTGAGCTCCAGATGTTGCCCAAGTCTCGGCTCAACACCGTCAATGTCAATTACTGTTCCATCAGCCAGGACTTCCCCAGCAGCAACGTGAATTTGCTCAACAACAATTCT
Peptide sequence:
MEQAPYGMQAPNYPKDNLLDSMCPPSATPAALGSELQMLPKSRLNTVNVNYCSISQDFPSSNVNLLNNNSENQEKTEDQYEENSHLNAVPHWINSNTLGFSMEQAPYGMQAPNYPKDNLLDSMCPPSATPAALGSELQMLPKSRLNTVNVNYCSISQDFPSSNVNLLNNNS
IRESfinder result
IRESfinder score
0.410394948
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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