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mmu_circEsyt2_261
Basic information of circular RNA
PanCircBase ID:
mmu_circEsyt2_261
mm39_Chr. Position ID:
chr12|116311345|116320806|+
Gene name:
Esyt2
Transcript:
ENSMUST00000100986.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
12,13,14
Exon/intron offsets:
0,493,9386
Genomic length:
9461
Exon/intron sizes in nt:
90,96,75
Flanking exon/introns:
chr12:116310172-116311345|chr12:116320806-116321467
Splice length:
261
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TGGTTCACTCTGGACGAGGTTCCCAAAGGAAAGCTGCATTTGAAGTTAGAGTGGCTCACACTGATGCCTGATGCTGCAAACCTTGACAAGGTGCTGGCAGACATCAGAGCTGACAAGGACCAAGCCAGTGATGGCCTTTCATCTGCACTGCTGATCTTGTATTTGGATTCTGCAAGGAACCTTCCGTCAGGGAAGAAAATAAACAGCAACCCAAATCCTCTTGTCCAGATGTCAGTCGGTCACAAGGCTCAAGAGAGTAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAACCCAAATCCTCTTGTCC
59.45
CAAGGTTTGCAGCATCAGGC
60.39
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCAACCCAAATCCTCTTGTCC
59.45
CCTTGTCAAGGTTTGCAGCA
59.25
147
PCR template sequence:
TTTGGATTCTGCAAGGAACCTTCCGTCAGGGAAGAAAATAAACAGCAACCCAAATCCTCTTGTCCAGATGTCAGTCGGTCACAAGGCTCAAGAGAGTAAGTGGTTCACTCTGGACGAGGTTCCCAAAGGAAAGCTGCATTTGAAGTTAGAGTGGCTCACACTGATGCCTGATGCTGCAAACCTTGACAAGGTGCTGGCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-152-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TCAAGAGAGTAAGTGGTTCACTCTGG
siRNA sets
GAACCACTTACTCTCTTGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCESYT2_261_ORF_1
1.1781
0.232237133
0.768052731
459
ORF sequence:
ATGCCTGATGCTGCAAACCTTGACAAGGTGCTGGCAGACATCAGAGCTGACAAGGACCAAGCCAGTGATGGCCTTTCATCTGCACTGCTGATCTTGTATTTGGATTCTGCAAGGAACCTTCCGTCAGGGAAGAAAATAAACAGCAACCCAAATCCTCTTGTCCAGATGTCAGTCGGTCACAAGGCTCAAGAGAGTAAGTGGTTCACTCTGGACGAGGTTCCCAAAGGAAAGCTGCATTTGAAGTTAGAGTGGCTCACACTGATGCCTGATGCTGCAAACCTTGACAAGGTGCTGGCAGACATCAGAGCTGACAAGGACCAAGCCAGTGATGGCCTTTCATCTGCACTGCTGATCTTGTATTTGGATTCTGCAAGGAACCTTCCGTCAGGGAAGAAAATAAACAGCAACCCAAATCCTCTTGTCCAGATGTCAGTCGGTCACAAGGCTCAAGAGAGTAAG
Peptide sequence:
MPDAANLDKVLADIRADKDQASDGLSSALLILYLDSARNLPSGKKINSNPNPLVQMSVGHKAQESKWFTLDEVPKGKLHLKLEWLTLMPDAANLDKVLADIRADKDQASDGLSSALLILYLDSARNLPSGKKINSNPNPLVQMSVGHKAQESK
IRESfinder result
IRESfinder score
0.306908094
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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