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mmu_circEro1b_496
Basic information of circular RNA
PanCircBase ID:
mmu_circEro1b_496
mm39_Chr. Position ID:
chr13|12605865|12616694|+
Gene name:
Ero1b
Transcript:
ENSMUST00000221560.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
8,9,10,11,12,13
Exon/intron offsets:
0,2523,4713,8884,9110,10759
Genomic length:
10829
Exon/intron sizes in nt:
47,12,27,93,247,70
Flanking exon/introns:
chr13:12605762-12605865|chr13:12616694-12617199
Splice length:
496
Other database IDs:
circAtlas: hsa-ERO1B_0017, CIRCpedia: HSA_CIRCpedia_174568
Human Ortholog IDs:
Mature spliced Sequence:
GCCTCGATCTGTTTATCGTCCTTTAAATCCTTTGGCGCCCAGCAGAGGGGAAGATGATGGAGAATCATTCTATACGTGGCTAGAAGGTTTGTGTCTTGAGAAAAGAGTCTTCTATAAGCTTATATCAGGACTCCATGCCAGCATCAATTTACATCTGTGTGCAAACTACCTTCTGGAAGAAACCTGGGGGAAACCTAGTTGGGGACCAAACATCAAGGAGTTTAGACGCCGCTTTGACCCTGTGGAAACAAAGGGGGAAGGTCCAAGGAGGCTAAAGAACCTGTACTTTTTATACTTGATAGAGCTCCGTGCTTTGTCAAAGGTGGCCCCTTACTTTGAGCGCTCGATTGTTGATCTCTATACTGGCAATGTGGAAGATGATGCCGACACCAAGACCCTTCTGCTCAGCATCTTTCAGGATACAAAGTCCTTTCCTATGCACTTCGATGAGAAATCCATGTTTGCAGGTGACAAAAAGGGGGCCAAGTCATTAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCTGCTCAGCATCTTTCAGGA
59.1
TCATCTTCCCCTCTGCTGGG
60.98
154
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCTGCTCAGCATCTTTCAGGA
59.1
CTCCATCATCTTCCCCTCTGC
59.93
159
PCR template sequence:
CCTTCTGCTCAGCATCTTTCAGGATACAAAGTCCTTTCCTATGCACTTCGATGAGAAATCCATGTTTGCAGGTGACAAAAAGGGGGCCAAGTCATTAAAGGCCTCGATCTGTTTATCGTCCTTTAAATCCTTTGGCGCCCAGCAGAGGGGAAGATGATGGAGAATCATTCTATACGTGGCTAGAAGGTTTGTGTCTTGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-26a-5p(1), mmu-miR-196a-5p(1), mmu-miR-26b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CAAGTCATTAAAGGCCTCGATCTGTT
siRNA sets
ATCGAGGCCTTTAATGACT,GATCGAGGCCTTTAATGAC,AGATCGAGGCCTTTAATGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCERO1B_496_ORF_5
0.9193
0.06446085
0.079521067
117
ORF sequence:
ATGCACTTCGATGAGAAATCCATGTTTGCAGGTGACAAAAAGGGGGCCAAGTCATTAAAGGCCTCGATCTGTTTATCGTCCTTTAAATCCTTTGGCGCCCAGCAGAGGGGAAGATGA
Peptide sequence:
MHFDEKSMFAGDKKGAKSLKASICLSSFKSFGAQQRGR
IRESfinder result
IRESfinder score
0.690651642
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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