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mmu_circErn1_2045
Basic information of circular RNA
PanCircBase ID:
mmu_circErn1_2045
mm39_Chr. Position ID:
chr11|106294273|106314314|-
Gene name:
Ern1
Transcript:
ENSMUST00000001059.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
13
Exon/intron indexes:
19,18,17,16,15,14,13,12,11,10,9,8,7
Exon/intron offsets:
0,2274,3593,4048,4612,5442,6447,8175,10903,15376,16571,18258,19939
Genomic length:
20041
Exon/intron sizes in nt:
12,81,48,20,01,00,19,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr11:106291144-106294273|chr11:106314314-106317638
Splice length:
2045
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AATACACCATCACCATGTATGACACCAAGACCCGGGAGCTCCGCTGGAATGCCACCTATTTTGACTATGCAGCCTCACTGCCGGAAGACGACGTGGACTACAAGATGTCCCACTTTGTGTCCAATGGCGATGGACTGGTGGTAACTGTGGACAGTGAATCTGGGGATGTCCTGTGGATCCAAAACTATGCCTCTCCTGTGGTGGCCTTCTACGTCTGGCAGGGGGAGGTCCTGAGAAAGGTGGTGCACATCAACGTTGCTGTGGAGACTCTACGCTACTTGACCTTCATGTCTGGGGAAGTGGGGCGCATCACCAAGTGGAAGTATCCATTCCCCAAGGAGACAGAGGCCAAGAGCAAGCTAACGCCTACTCTGTATGTTGGGAAGTATTCCACCAGCCTCTATGCCTCTCCCTCAATGGTGCATGAGGGGGTTGCTGTCGTGCCTCGAGGCAGCACTCTTCCTTTGCTGGAAGGCCCCCAGACAGATGGCGTCACCATTGGAGACAAAGGAGAGTGTGTGATCACTCCCAGCACAGACCTCAAGTTTGACCCTGGACTCAAAGGGAAGAGCAAGCTGAACTACTTGAGGAATTACTGGCTTCTCATAGGACACCATGAAACTCCTCTGTCTGCATCCACCAAGATGCTGGAGAGATTTCCTAACAACCTGCCCAAACATCGAGAAAATGTGATTCCTGCTGATTCAGAAAAAAGGAGCTTTGAGGAAGTTATCAACATAGTTGGCCAGACTTCAGACAACACACCGACCACCGTATCTCAGGATGTGGAGGAGAAGCTCGCTCGCGCCCCTGCCAAGCCTGAGGCCCCCGTGGACTCCATGCTCAAGGACATGGCTACCATTATCCTGAGCACCTTCCTGCTGGTTGGATGGGTGGCGTTCATCATCACTTACCCCCTGAGCGTGCATCAGCAGCGTCAGCTCCAGCACCAACAGTTCCAGAAGGAGCTGGAGAAGATTCAGCTCCTGCAGCAGCAGCAGCTGCCCTTCCACCCACACGGAGACCTTACCCAGGACCCTGAGTTCCTGGATTCATCTGGCCCCTTCTCAGAGAGCTCTGGCACCAGCAGCCCCAGCCCATCCCCCAGAGCCTCCAACCACTCCCTCCACCCCAGCAGCTCTGCCTCCAGGGCCGGCACCAGCCCCTCTCTGGAGCAGGATGATGAGGATGAGGAAACCAGAATGGTGATTGTTGGGAAAATTTCATTCTGCCCCAAGGATGTCCTGGGTCATGGAGCTGAGGGCACAATTGTATACAAAGGTATGTTTGACAACCGAGATGTGGCCGTGAAGAGGATCCTCCCTGAGTGTTTTAGCTTTGCCGACCGTGAGGTCCAGCTGCTTCGAGAATCAGACGAGCACCCAAATGTGATCCGCTACTTTTGCACAGAGAAGGACCGGCAGTTCCAGTACATTGCTATCGAGCTGTGTGCAGCCACCCTACAAGAGTATGTGGAGCAGAAGGACTTTGCCCACCTTGGCCTCGAGCCCATCACCCTGCTTCATCAGACCACCTCAGGCCTGGCACACCTGCATTCTCTCAACATTGTTCACAGAGACCTGAAGCCCCACAACATTCTCCTCTCCATGCCCAACGCACATGGCAGGATCAAGGCGATGATCTCTGACTTTGGCCTCTGCAAGAAGCTGGCAGTGGGCAGGCACAGTTTCAGCCGCCGTTCAGGGGTACCTGGCACTGAAGGGTGGATCGCCCCAGAGATGCTGAGTGAAGACTGTAAGGACAACCCTACCTACACGGTGGACATCTTTTCTGCAGGCTGTGTCTTTTACTATGTCATCTCTGAGGGCAACCATCCTTTTGGCAAATCCTTGCAGCGGCAGGCCAACATCCTCCTGGGCGCCTGCAACCTTGACTGTTTCCACTCAGACAAGCATGAGGACGTCATTGCTCGTGAATTGATAGAGAAAATGATTGCTATGGATCCCCAGCAGCGTCCCTCTGCAAAGCACGTGCTGAAACACCCCTTCTTCTGGAGCCTGGAGAAGCAGCTCCAGTTTTTCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AACACCCCTTCTTCTGGAGC
59.6
TAGTCCACGTCGTCTTCCGG
61.3
147
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGTGCTGAAACACCCCTTCT
60.54
TAGTCCACGTCGTCTTCCGG
61.3
155
PCR template sequence:
GAAAATGATTGCTATGGATCCCCAGCAGCGTCCCTCTGCAAAGCACGTGCTGAAACACCCCTTCTTCTGGAGCCTGGAGAAGCAGCTCCAGTTTTTCCAGAATACACCATCACCATGTATGACACCAAGACCCGGGAGCTCCGCTGGAATGCCACCTATTTTGACTATGCAGCCTCACTGCCGGAAGACGACGTGGACTA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
13
Interacting miRNAs (#binding sites)
mmu-miR-207(3), mmu-miR-298-5p(2), mmu-miR-140-5p(1), mmu-miR-26a-5p(1), mmu-miR-709(1), mmu-miR-326-3p(1), mmu-miR-153-3p(1), mmu-miR-26b-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-182-5p(1), mmu-miR-370-3p(1), mmu-miR-697(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
CCAGTTTTTCCAGAATACACCATCAC
siRNA sets
TGTATTCTGGAAAAACTGG, GTGTATTCTGGAAAAACTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCERN1_2045_ORF_1
1.1364
0.359917951
0.999999119
2175
ORF sequence:
ATGTATGACACCAAGACCCGGGAGCTCCGCTGGAATGCCACCTATTTTGACTATGCAGCCTCACTGCCGGAAGACGACGTGGACTACAAGATGTCCCACTTTGTGTCCAATGGCGATGGACTGGTGGTAACTGTGGACAGTGAATCTGGGGATGTCCTGTGGATCCAAAACTATGCCTCTCCTGTGGTGGCCTTCTACGTCTGGCAGGGGGAGGTCCTGAGAAAGGTGGTGCACATCAACGTTGCTGTGGAGACTCTACGCTACTTGACCTTCATGTCTGGGGAAGTGGGGCGCATCACCAAGTGGAAGTATCCATTCCCCAAGGAGACAGAGGCCAAGAGCAAGCTAACGCCTACTCTGTATGTTGGGAAGTATTCCACCAGCCTCTATGCCTCTCCCTCAATGGTGCATGAGGGGGTTGCTGTCGTGCCTCGAGGCAGCACTCTTCCTTTGCTGGAAGGCCCCCAGACAGATGGCGTCACCATTGGAGACAAAGGAGAGTGTGTGATCACTCCCAGCACAGACCTCAAGTTTGACCCTGGACTCAAAGGGAAGAGCAAGCTGAACTACTTGAGGAATTACTGGCTTCTCATAGGACACCATGAAACTCCTCTGTCTGCATCCACCAAGATGCTGGAGAGATTTCCTAACAACCTGCCCAAACATCGAGAAAATGTGATTCCTGCTGATTCAGAAAAAAGGAGCTTTGAGGAAGTTATCAACATAGTTGGCCAGACTTCAGACAACACACCGACCACCGTATCTCAGGATGTGGAGGAGAAGCTCGCTCGCGCCCCTGCCAAGCCTGAGGCCCCCGTGGACTCCATGCTCAAGGACATGGCTACCATTATCCTGAGCACCTTCCTGCTGGTTGGATGGGTGGCGTTCATCATCACTTACCCCCTGAGCGTGCATCAGCAGCGTCAGCTCCAGCACCAACAGTTCCAGAAGGAGCTGGAGAAGATTCAGCTCCTGCAGCAGCAGCAGCTGCCCTTCCACCCACACGGAGACCTTACCCAGGACCCTGAGTTCCTGGATTCATCTGGCCCCTTCTCAGAGAGCTCTGGCACCAGCAGCCCCAGCCCATCCCCCAGAGCCTCCAACCACTCCCTCCACCCCAGCAGCTCTGCCTCCAGGGCCGGCACCAGCCCCTCTCTGGAGCAGGATGATGAGGATGAGGAAACCAGAATGGTGATTGTTGGGAAAATTTCATTCTGCCCCAAGGATGTCCTGGGTCATGGAGCTGAGGGCACAATTGTATACAAAGGTATGTTTGACAACCGAGATGTGGCCGTGAAGAGGATCCTCCCTGAGTGTTTTAGCTTTGCCGACCGTGAGGTCCAGCTGCTTCGAGAATCAGACGAGCACCCAAATGTGATCCGCTACTTTTGCACAGAGAAGGACCGGCAGTTCCAGTACATTGCTATCGAGCTGTGTGCAGCCACCCTACAAGAGTATGTGGAGCAGAAGGACTTTGCCCACCTTGGCCTCGAGCCCATCACCCTGCTTCATCAGACCACCTCAGGCCTGGCACACCTGCATTCTCTCAACATTGTTCACAGAGACCTGAAGCCCCACAACATTCTCCTCTCCATGCCCAACGCACATGGCAGGATCAAGGCGATGATCTCTGACTTTGGCCTCTGCAAGAAGCTGGCAGTGGGCAGGCACAGTTTCAGCCGCCGTTCAGGGGTACCTGGCACTGAAGGGTGGATCGCCCCAGAGATGCTGAGTGAAGACTGTAAGGACAACCCTACCTACACGGTGGACATCTTTTCTGCAGGCTGTGTCTTTTACTATGTCATCTCTGAGGGCAACCATCCTTTTGGCAAATCCTTGCAGCGGCAGGCCAACATCCTCCTGGGCGCCTGCAACCTTGACTGTTTCCACTCAGACAAGCATGAGGACGTCATTGCTCGTGAATTGATAGAGAAAATGATTGCTATGGATCCCCAGCAGCGTCCCTCTGCAAAGCACGTGCTGAAACACCCCTTCTTCTGGAGCCTGGAGAAGCAGCTCCAGTTTTTCCAGAATACACCATCACCATGTATGACACCAAGACCCGGGAGCTCCGCTGGAATGCCACCTATTTTGACTATGCAGCCTCACTGCCGGAAGACGACGTGGACTACAAGATGTCCCACTTTGTGTCCAATGGCGATGGACTGGTGGTAA
Peptide sequence:
MYDTKTRELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVTVDSESGDVLWIQNYASPVVAFYVWQGEVLRKVVHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDLKFDPGLKGKSKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKRSFEEVINIVGQTSDNTPTTVSQDVEEKLARAPAKPEAPVDSMLKDMATIILSTFLLVGWVAFIITYPLSVHQQRQLQHQQFQKELEKIQLLQQQQLPFHPHGDLTQDPEFLDSSGPFSESSGTSSPSPSPRASNHSLHPSSSASRAGTSPSLEQDDEDEETRMVIVGKISFCPKDVLGHGAEGTIVYKGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLHQTTSGLAHLHSLNIVHRDLKPHNILLSMPNAHGRIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKDNPTYTVDIFSAGCVFYYVISEGNHPFGKSLQRQANILLGACNLDCFHSDKHEDVIARELIEKMIAMDPQQRPSAKHVLKHPFFWSLEKQLQFFQNTPSPCMTPRPGSSAGMPPILTMQPHCRKTTWTTRCPTLCPMAMDWW
IRESfinder result
IRESfinder score
0.499808471
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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