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mmu_circErlec1_1113
Basic information of circular RNA
PanCircBase ID:
mmu_circErlec1_1113
mm39_Chr. Position ID:
chr11|30884671|30901387|-
Gene name:
Erlec1
Transcript:
ENSMUST00000073192.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
11
Exon/intron indexes:
13,12,11,10,9,8,7,6,5,4,3
Exon/intron offsets:
0,310,4825,8367,9027,13407,13628,15271,15888,16069,16635
Genomic length:
16716
Exon/intron sizes in nt:
76,78,125,60,162,130,224,35,64,78,81
Flanking exon/introns:
chr11:30881848-30884671|chr11:30901387-30902571
Splice length:
1113
Other database IDs:
circAtlas: mmu-Erlec1_0001
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
GAAGAAGAAAAGGATTACAAAGGCCCCAATCCAAGAGAGCTTTTGGAACCACTATTTAAACAAAGCAGTTGTTCCTACAGAATTGAATCTTACTGGACTTATGAGGTCTGTCATGGGAAACATATTCGACAGTACCATGAAGAAAAAGAAACTGGCCAAAAAGTAAATATTCATGAATACTACCTTGGGAATATGTTGGCTAAGAATCTTCTGTATGAAAAAGAACGAGAAGCAAAAGAAAATGAAAAGTCAAATGAGATTCCCACTAAAAACATTGAAGGTCAGATGACACCCTACTATCCTGTTGGAATGGGAAATGGTACACCTTGTAGTCTGAAACAAAATCGACCCAGATCAAGTACTGTGATGTACATATGCCATCCTGAATCTAAGCATGAGATCCTTTCAGTAGCTGAAGTTACAACTTGTGAATATGAAGTTGTCATTTTGACACCACTCTTGTGCAGTCATCCTAAATACAAGTTCAGAGCCTCTCCTGTGAATGACATATTCTGTCAATCACTGCCGGGCTCTCCATTTAAACCCCTCACACTAAGACAGTTGGAGCAGCAGGAAGAGATACTGAGAGTGCCATTCAGGAGAAATAAAGAGGAAGATTTGCCATCTGCTAAGGAAGAGAGATTTCCAGCCATCCACAAACCTATTGCTGTTGGCTCTCAGCCAGTGCTCACTGTTGGAACAACCCACATTTCCAAGCTGACAGATGACCAGCTCATCAAGGAGTTCCTAAGTGGCTCTTACTGCTTTCATGGGGGTGTTGGTTGGTGGAAATATGAATTCTGCTATGGCAAACATGTACATCAATATCACGAGGACAAAGATAATGGAAAAACTTCTGTAGTTGTGGGGACATGGAACCAAGAAGAGCATGTTGAGTGGGCTAAGAAAAATACTGCTAGAGCGTATCATCTTCAAGACGATGGCACCCAGACAGTCAGGATGGTGTCACATTTTTATGGAAATGGGGATATTTGTGATATAACTGACAAACCCAGACAAGTGACTGTAAAACTAAAGTGTAAAGAATCAGACTCTCCTCATGCTGTCACTGTGTATATGCTAGAGCCTCACTCGTGCCAGTATATTCTTGGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTCCTCATGCTGTCACTGTGT
60
TCTGTAGGAACAACTGCTTTG
56.11
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCCTCATGCTGTCACTGTGT
60
TCAATTCTGTAGGAACAACTGCT
58.03
145
PCR template sequence:
CAGACAAGTGACTGTAAAACTAAAGTGTAAAGAATCAGACTCTCCTCATGCTGTCACTGTGTATATGCTAGAGCCTCACTCGTGCCAGTATATTCTTGGGGAAGAAGAAAAGGATTACAAAGGCCCCAATCCAAGAGAGCTTTTGGAACCACTATTTAAACAAAGCAGTTGTTCCTACAGAATTGAATCTTACTGGACTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(1), mmu-miR-223-3p(1), mmu-miR-101a-3p(1), mmu-miR-216a-5p(1), mmu-miR-196a-5p(1), mmu-miR-143-3p(1), mmu-miR-382-3p(1), mmu-miR-183-5p(1), mmu-miR-34b-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-335-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GTATATTCTTGGGGAAGAAGAAAAGG
siRNA sets
CTTTTCTTCTTCCCCAAGA, TTTTCTTCTTCCCCAAGAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCERLEC1_1113_ORF_1
0.9947
0.039248672
0.999988728
2034
ORF sequence:
ATGTTGGCTAAGAATCTTCTGTATGAAAAAGAACGAGAAGCAAAAGAAAATGAAAAGTCAAATGAGATTCCCACTAAAAACATTGAAGGTCAGATGACACCCTACTATCCTGTTGGAATGGGAAATGGTACACCTTGTAGTCTGAAACAAAATCGACCCAGATCAAGTACTGTGATGTACATATGCCATCCTGAATCTAAGCATGAGATCCTTTCAGTAGCTGAAGTTACAACTTGTGAATATGAAGTTGTCATTTTGACACCACTCTTGTGCAGTCATCCTAAATACAAGTTCAGAGCCTCTCCTGTGAATGACATATTCTGTCAATCACTGCCGGGCTCTCCATTTAAACCCCTCACACTAAGACAGTTGGAGCAGCAGGAAGAGATACTGAGAGTGCCATTCAGGAGAAATAAAGAGGAAGATTTGCCATCTGCTAAGGAAGAGAGATTTCCAGCCATCCACAAACCTATTGCTGTTGGCTCTCAGCCAGTGCTCACTGTTGGAACAACCCACATTTCCAAGCTGACAGATGACCAGCTCATCAAGGAGTTCCTAAGTGGCTCTTACTGCTTTCATGGGGGTGTTGGTTGGTGGAAATATGAATTCTGCTATGGCAAACATGTACATCAATATCACGAGGACAAAGATAATGGAAAAACTTCTGTAGTTGTGGGGACATGGAACCAAGAAGAGCATGTTGAGTGGGCTAAGAAAAATACTGCTAGAGCGTATCATCTTCAAGACGATGGCACCCAGACAGTCAGGATGGTGTCACATTTTTATGGAAATGGGGATATTTGTGATATAACTGACAAACCCAGACAAGTGACTGTAAAACTAAAGTGTAAAGAATCAGACTCTCCTCATGCTGTCACTGTGTATATGCTAGAGCCTCACTCGTGCCAGTATATTCTTGGGGAAGAAGAAAAGGATTACAAAGGCCCCAATCCAAGAGAGCTTTTGGAACCACTATTTAAACAAAGCAGTTGTTCCTACAGAATTGAATCTTACTGGACTTATGAGGTCTGTCATGGGAAACATATTCGACAGTACCATGAAGAAAAAGAAACTGGCCAAAAAGTAAATATTCATGAATACTACCTTGGGAATATGTTGGCTAAGAATCTTCTGTATGAAAAAGAACGAGAAGCAAAAGAAAATGAAAAGTCAAATGAGATTCCCACTAAAAACATTGAAGGTCAGATGACACCCTACTATCCTGTTGGAATGGGAAATGGTACACCTTGTAGTCTGAAACAAAATCGACCCAGATCAAGTACTGTGATGTACATATGCCATCCTGAATCTAAGCATGAGATCCTTTCAGTAGCTGAAGTTACAACTTGTGAATATGAAGTTGTCATTTTGACACCACTCTTGTGCAGTCATCCTAAATACAAGTTCAGAGCCTCTCCTGTGAATGACATATTCTGTCAATCACTGCCGGGCTCTCCATTTAAACCCCTCACACTAAGACAGTTGGAGCAGCAGGAAGAGATACTGAGAGTGCCATTCAGGAGAAATAAAGAGGAAGATTTGCCATCTGCTAAGGAAGAGAGATTTCCAGCCATCCACAAACCTATTGCTGTTGGCTCTCAGCCAGTGCTCACTGTTGGAACAACCCACATTTCCAAGCTGACAGATGACCAGCTCATCAAGGAGTTCCTAAGTGGCTCTTACTGCTTTCATGGGGGTGTTGGTTGGTGGAAATATGAATTCTGCTATGGCAAACATGTACATCAATATCACGAGGACAAAGATAATGGAAAAACTTCTGTAGTTGTGGGGACATGGAACCAAGAAGAGCATGTTGAGTGGGCTAAGAAAAATACTGCTAGAGCGTATCATCTTCAAGACGATGGCACCCAGACAGTCAGGATGGTGTCACATTTTTATGGAAATGGGGATATTTGTGATATAACTGACAAACCCAGACAAGTGACTGTAAAACTAAAGTGTAAAGAATCAGACTCTCCTCATGCTGTCACTGTGTATATGCTAGAGCCTCACTCGTGCCAGTATATTCTTGGG
Peptide sequence:
MLAKNLLYEKEREAKENEKSNEIPTKNIEGQMTPYYPVGMGNGTPCSLKQNRPRSSTVMYICHPESKHEILSVAEVTTCEYEVVILTPLLCSHPKYKFRASPVNDIFCQSLPGSPFKPLTLRQLEQQEEILRVPFRRNKEEDLPSAKEERFPAIHKPIAVGSQPVLTVGTTHISKLTDDQLIKEFLSGSYCFHGGVGWWKYEFCYGKHVHQYHEDKDNGKTSVVVGTWNQEEHVEWAKKNTARAYHLQDDGTQTVRMVSHFYGNGDICDITDKPRQVTVKLKCKESDSPHAVTVYMLEPHSCQYILGEEEKDYKGPNPRELLEPLFKQSSCSYRIESYWTYEVCHGKHIRQYHEEKETGQKVNIHEYYLGNMLAKNLLYEKEREAKENEKSNEIPTKNIEGQMTPYYPVGMGNGTPCSLKQNRPRSSTVMYICHPESKHEILSVAEVTTCEYEVVILTPLLCSHPKYKFRASPVNDIFCQSLPGSPFKPLTLRQLEQQEEILRVPFRRNKEEDLPSAKEERFPAIHKPIAVGSQPVLTVGTTHISKLTDDQLIKEFLSGSYCFHGGVGWWKYEFCYGKHVHQYHEDKDNGKTSVVVGTWNQEEHVEWAKKNTARAYHLQDDGTQTVRMVSHFYGNGDICDITDKPRQVTVKLKCKESDSPHAVTVYMLEPHSCQYILG
IRESfinder result
IRESfinder score
0.573675327
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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