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mmu_circEps15l1_234
Basic information of circular RNA
PanCircBase ID:
mmu_circEps15l1_234
mm39_Chr. Position ID:
chr8|73150745|73153610|-
Gene name:
Eps15l1
Transcript:
ENSMUST00000163643.3
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
5,4,3
Exon/intron offsets:
0,2104,2775
Genomic length:
2865
Exon/intron sizes in nt:
96,48,90
Flanking exon/introns:
chr8:73150140-73150745|chr8:73153610-73154035
Splice length:
234
Other database IDs:
circAtlas: hsa-EPS15L1_0056
Human Ortholog IDs:
Mature spliced Sequence:
GTGGACCCTGCCTACACAGGGAGAGTTGGGGCAAGCGAGGCTGCGCTGTTTCTGAAGAAATCGGGGCTCTCAGACATCATCCTGGGGAAGATATGGGACTTGGCAGACCCCGAAGGTAAAGGGTTCTTGGACAAACAGGGTTTCTATGTTGCACTGCGACTGGTGGCCTGTGCACAGAGTGGCCATGAAGTCACCTTGAGCAGTCTGAGCCTCACCATGCCACCACCAAAATTT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TATGTTGCACTGCGACTGGT
59.97
GATGATGTCTGAGAGCCCCG
59.97
171
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGAGTGGCCATGAAGTCACC
59.67
GATGATGTCTGAGAGCCCCG
59.97
140
PCR template sequence:
ACAGGGTTTCTATGTTGCACTGCGACTGGTGGCCTGTGCACAGAGTGGCCATGAAGTCACCTTGAGCAGTCTGAGCCTCACCATGCCACCACCAAAATTTGTGGACCCTGCCTACACAGGGAGAGTTGGGGCAAGCGAGGCTGCGCTGTTTCTGAAGAAATCGGGGCTCTCAGACATCATCCTGGGGAAGATATGGGACT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-130a-3p(1), mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-10a-5p(1), mmu-miR-130b-3p(1), mmu-miR-721(1), mmu-miR-3470b(1)
siRNAs for circular RNA silencing
Junction sequence
ACCACCAAAATTTGTGGACCCTGCCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEPS15L1_234_ORF_1
0.9107
0.265769313
0.341781233
252
ORF sequence:
ATGCCACCACCAAAATTTGTGGACCCTGCCTACACAGGGAGAGTTGGGGCAAGCGAGGCTGCGCTGTTTCTGAAGAAATCGGGGCTCTCAGACATCATCCTGGGGAAGATATGGGACTTGGCAGACCCCGAAGGTAAAGGGTTCTTGGACAAACAGGGTTTCTATGTTGCACTGCGACTGGTGGCCTGTGCACAGAGTGGCCATGAAGTCACCTTGAGCAGTCTGAGCCTCACCATGCCACCACCAAAATTT
Peptide sequence:
MPPPKFVDPAYTGRVGASEAALFLKKSGLSDIILGKIWDLADPEGKGFLDKQGFYVALRLVACAQSGHEVTLSSLSLTMPPPKF
IRESfinder result
IRESfinder score
0.500898551
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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