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mmu_circEprs_482
Basic information of circular RNA
PanCircBase ID:
mmu_circEprs_482
mm39_Chr. Position ID:
chr1|185099727|185105254|+
Gene name:
Eprs
Transcript:
ENSMUST00000192284.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
2,3,4,5
Exon/intron offsets:
0,2046,3358,5387
Genomic length:
5527
Exon/intron sizes in nt:
85,10,01,57,140
Flanking exon/introns:
chr1:185095440-185099727|chr1:185105254-185106314
Splice length:
482
Other database IDs:
CIRCpedia: MMU_CIRCpedia_12528
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_113957
Human Ortholog IDs:
Mature spliced Sequence:
AAGCTCTGCTGGCGGTGGAACATGTGAAAGGTGATGTCAGCATTTCTGTGGAAGAAGGGAAAGAGAATCTTCTTCGGGTTTCTGAGACTGTGGCATTCACTGACGTGAATTCAATCCTGCGCTACCTGGCTAGAATTGCAACTACGTCTGGGCTGTATGGGACTAACCTGATGGAGCACACTGAGATTGATCATTGGTTGGAGTTTAGTGCTACAAAGTTGTCTTCGTGTGATAGGCTGACTTCTGCAATCAACGAGCTTAATCATTGCCTGTCTCTGAGAACATACCTAGTTGGGAACTCCTTGACCTTAGCAGATCTATGTGTTTGGGCCACCCTAAAAGGAAGTGCTGCATGGCAGGAACACTTGAAACAGAACAAAACTCTAGTCCACGTTAAACGCTGGTTTGGCTTTCTTGAGGCCCAGCAGGCCTTCCGCTCAGTTGGCACCAAGTGGGATGTTTCAGGAAACAGAGCTACAGTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTTGGCTTTCTTGAGGCCCA
60.11
TGCCACAGTCTCAGAAACCC
59.89
173
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTTTGGCTTTCTTGAGGCCC
59.68
TGCCACAGTCTCAGAAACCC
59.89
174
PCR template sequence:
TCTAGTCCACGTTAAACGCTGGTTTGGCTTTCTTGAGGCCCAGCAGGCCTTCCGCTCAGTTGGCACCAAGTGGGATGTTTCAGGAAACAGAGCTACAGTGAAGCTCTGCTGGCGGTGGAACATGTGAAAGGTGATGTCAGCATTTCTGTGGAAGAAGGGAAAGAGAATCTTCTTCGGGTTTCTGAGACTGTGGCATTCAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-34a-5p(1), mmu-miR-370-3p(1), mmu-miR-377-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CAGAGCTACAGTGAAGCTCTGCTGGC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEPRS_482_ORF_1
0.5431
0.000695887
0.14105829
339
ORF sequence:
ATGGAGCACACTGAGATTGATCATTGGTTGGAGTTTAGTGCTACAAAGTTGTCTTCGTGTGATAGGCTGACTTCTGCAATCAACGAGCTTAATCATTGCCTGTCTCTGAGAACATACCTAGTTGGGAACTCCTTGACCTTAGCAGATCTATGTGTTTGGGCCACCCTAAAAGGAAGTGCTGCATGGCAGGAACACTTGAAACAGAACAAAACTCTAGTCCACGTTAAACGCTGGTTTGGCTTTCTTGAGGCCCAGCAGGCCTTCCGCTCAGTTGGCACCAAGTGGGATGTTTCAGGAAACAGAGCTACAGTGAAGCTCTGCTGGCGGTGGAACATGTGA
Peptide sequence:
MEHTEIDHWLEFSATKLSSCDRLTSAINELNHCLSLRTYLVGNSLTLADLCVWATLKGSAAWQEHLKQNKTLVHVKRWFGFLEAQQAFRSVGTKWDVSGNRATVKLCWRWNM
IRESfinder result
IRESfinder score
0.601498032
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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