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mmu_circEprs_365
Basic information of circular RNA
PanCircBase ID:
mmu_circEprs_365
mm39_Chr. Position ID:
chr1|185111741|185114168|+
Gene name:
Eprs
Transcript:
ENSMUST00000192284.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
8,9
Exon/intron offsets:
0,2255
Genomic length:
2427
Exon/intron sizes in nt:
1,93,172
Flanking exon/introns:
chr1:185111098-185111741|chr1:185114168-185117073
Splice length:
365
Other database IDs:
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_170507
Human Ortholog IDs:
Mature spliced Sequence:
GTCATCTTGGAAGATGTTGCAATGCTGCATATTAAACCAGATCAGTTTACTTACACTTCAGATCATTTTGAAACTATAATGAAGTATGCAGAGAAATTAATTCAAGAAGGGAAGGCTTATGTGGATGACACTCCTGCAGAACAGATGAAGGCGGAACGTGAACAGAGGACAGAATCCAAACACAGGAAGAACTCTGTTGAGAAGAATCTACAGATGTGGGAAGAAATGAAAAAAGGAAGCCAGTTTGGTCAGTCCTGTTGCCTGCGAGCAAAAATTGACATGAGTAGTAACAATGGGTGTATGAGAGACCCGACCCTTTACCGATGCAAAATCCAGCCCCACCCAAGGACTGGGAATAAATACAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGTATGAGAGACCCGACCCT
59.37
AGTTTCAAAATGATCTGAAGTGTAAGT
57.87
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTGTATGAGAGACCCGACCC
59.25
AGTTTCAAAATGATCTGAAGTGTAAGT
57.87
144
PCR template sequence:
GAGCAAAAATTGACATGAGTAGTAACAATGGGTGTATGAGAGACCCGACCCTTTACCGATGCAAAATCCAGCCCCACCCAAGGACTGGGAATAAATACAAGTCATCTTGGAAGATGTTGCAATGCTGCATATTAAACCAGATCAGTTTACTTACACTTCAGATCATTTTGAAACTATAATGAAGTATGCAGAGAAATTAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-103-3p(1), mmu-miR-205-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-107-3p(1), mmu-miR-335-3p(1), mmu-miR-188-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGAATAAATACAAGTCATCTTGGAAG
siRNA sets
GATGACTTGTATTTATTCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEPRS_365_ORF_1
0.7507
0.03323837
0.337208767
423
ORF sequence:
ATGCTGCATATTAAACCAGATCAGTTTACTTACACTTCAGATCATTTTGAAACTATAATGAAGTATGCAGAGAAATTAATTCAAGAAGGGAAGGCTTATGTGGATGACACTCCTGCAGAACAGATGAAGGCGGAACGTGAACAGAGGACAGAATCCAAACACAGGAAGAACTCTGTTGAGAAGAATCTACAGATGTGGGAAGAAATGAAAAAAGGAAGCCAGTTTGGTCAGTCCTGTTGCCTGCGAGCAAAAATTGACATGAGTAGTAACAATGGGTGTATGAGAGACCCGACCCTTTACCGATGCAAAATCCAGCCCCACCCAAGGACTGGGAATAAATACAAGTCATCTTGGAAGATGTTGCAATGCTGCATATTAAACCAGATCAGTTTACTTACACTTCAGATCATTTTGAAACTATAA
Peptide sequence:
MLHIKPDQFTYTSDHFETIMKYAEKLIQEGKAYVDDTPAEQMKAEREQRTESKHRKNSVEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYKSSWKMLQCCILNQISLLTLQIILKL
IRESfinder result
IRESfinder score
0.508410046
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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