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mmu_circEpn2_1270
Basic information of circular RNA
PanCircBase ID:
mmu_circEpn2_1270
mm39_Chr. Position ID:
chr11|61423343|61456720|-
Gene name:
Epn2
Transcript:
ENSMUST00000178202.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
7,6,5,4,3,2
Exon/intron offsets:
0,1053,2060,2753,13632,33304
Genomic length:
33377
Exon/intron sizes in nt:
175,111,36,113,762,73
Flanking exon/introns:
chr11:61414019-61423343|chr11:61456720-61470392
Splice length:
1270
Other database IDs:
circAtlas: mmu-Epn2_0022
Human Ortholog IDs:
Mature spliced Sequence:
TTATGGCATTCCTTGCTCCAGGATTTGGGACAGGACGCTGATTTTGCACATCTTAGGACCCATTGTGGATAAGGGCATGGCTGCCAGCTTCTGGGACCTGAGGCTTTGGAGGAGGAAATGACTGTGGAGGGCCATTTTGTTCCATCTGCATTTCTGACACGGAAACTTCCTAGGGTGCAGGCTTAGGGGACAGGAAAGTTTCTCTTGAAGGGCTTCACATTTGTAACAAAGAAGATAAAAATGACAACTTCATCTATCAGACGGCAGATGAAAAACATTGTGAACAATTACTCAGAGGCTGAAATCAAAGTCCGGGAGGCCACCTCCAATGACCCGTGGGGCCCATCCAGCTCTCTGATGACTGAGATTGCTGACCTGACCTACAATGTGGTAGCGTTCTCGGAGATCATGAGCATGGTTTGGAAGCGACTTAATGACCATGGCAAGAACTGGCGGCATGTATACAAGGCACTGACACTGCTGGACTACCTTATCAAGACAGGTTCTGAGCGGGTGGCCCAGCAGTGCCGTGAGAACATCTTTGCCATACAGACTCTGAAGGACTTTCAGTACATTGACCGTGATGGCAAAGACCAGGGTATTAACGTTCGAGAGAAATCAAAGCAACTGGTTGCGCTCCTCAAGGATGAGGAGCGGCTGAAGGTTGAGAGAGTTCAGGCTCTCAAAACCAAAGAGCGCATGGCTCAGGTGGCCACTGGCGTGGGCAGCAACCAAATCACCTTTGGCCGTGGCTCCAGCCAGCCCAACCTTTCTACCAGCTACTCAGAGCAGGAGTATGGCAAGGCTGGGGGCTCGCCGGCATCCTACCACGGCTCTACTTCCCCACGAGTATCCTCTGAGTTAGAGCAGGCCCGGCCACAGACAAGCGGAGAAGAGGAGCTGCAGCTACAACTGGCACTTGCCATGAGCAGAGAGGTTGCTGAGCAGTCATCAGAAAGCGTGCAGACAGCCAGAGGCAGCAAGGAAGAACGCCTCAGGCGGGGTGATGACCTCAGATTGCAGATGGCTCTGGAAGAAAGCCGGAGAGACACAGTAAAAGTTCCAAAAAAGAAAGAGGCGAAAGCTTGTTGCAAGCCAGGCTCCCACTCCCAGCAGACTACCTTGTTGGATTTAATGGATGCCCTCCCCAGCTCAGGCCCTGTTACACAGAAAACTGAGCCCTGGAGTGCAGGAGCCTCTGCTAACCAGACCAACCCCTGGGGTGGAACAGTGGCTCCTTCGAACATTACTGACCCGTGGCCATCGTTTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCTAACCAGACCAACCCCTG
60.32
CAGCCATGCCCTTATCCACA
60.11
153
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTGCTAACCAGACCAACCCC
60.32
CAGCCATGCCCTTATCCACA
60.11
155
PCR template sequence:
AAAACTGAGCCCTGGAGTGCAGGAGCCTCTGCTAACCAGACCAACCCCTGGGGTGGAACAGTGGCTCCTTCGAACATTACTGACCCGTGGCCATCGTTTGTTATGGCATTCCTTGCTCCAGGATTTGGGACAGGACGCTGATTTTGCACATCTTAGGACCCATTGTGGATAAGGGCATGGCTGCCAGCTTCTGGGACCTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-196a-5p(2), mmu-miR-9-5p(1), mmu-miR-328-3p(1), mmu-miR-298-5p(1), mmu-miR-216a-5p(1), mmu-miR-24-3p(1), mmu-miR-370-3p(1), mmu-miR-335-3p(1), mmu-miR-452-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGGCCATCGTTTGTTATGGCATTCCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEPN2_1270_ORF_1
0.9184
0.327663864
0.995822155
1071
ORF sequence:
ATGACAACTTCATCTATCAGACGGCAGATGAAAAACATTGTGAACAATTACTCAGAGGCTGAAATCAAAGTCCGGGAGGCCACCTCCAATGACCCGTGGGGCCCATCCAGCTCTCTGATGACTGAGATTGCTGACCTGACCTACAATGTGGTAGCGTTCTCGGAGATCATGAGCATGGTTTGGAAGCGACTTAATGACCATGGCAAGAACTGGCGGCATGTATACAAGGCACTGACACTGCTGGACTACCTTATCAAGACAGGTTCTGAGCGGGTGGCCCAGCAGTGCCGTGAGAACATCTTTGCCATACAGACTCTGAAGGACTTTCAGTACATTGACCGTGATGGCAAAGACCAGGGTATTAACGTTCGAGAGAAATCAAAGCAACTGGTTGCGCTCCTCAAGGATGAGGAGCGGCTGAAGGTTGAGAGAGTTCAGGCTCTCAAAACCAAAGAGCGCATGGCTCAGGTGGCCACTGGCGTGGGCAGCAACCAAATCACCTTTGGCCGTGGCTCCAGCCAGCCCAACCTTTCTACCAGCTACTCAGAGCAGGAGTATGGCAAGGCTGGGGGCTCGCCGGCATCCTACCACGGCTCTACTTCCCCACGAGTATCCTCTGAGTTAGAGCAGGCCCGGCCACAGACAAGCGGAGAAGAGGAGCTGCAGCTACAACTGGCACTTGCCATGAGCAGAGAGGTTGCTGAGCAGTCATCAGAAAGCGTGCAGACAGCCAGAGGCAGCAAGGAAGAACGCCTCAGGCGGGGTGATGACCTCAGATTGCAGATGGCTCTGGAAGAAAGCCGGAGAGACACAGTAAAAGTTCCAAAAAAGAAAGAGGCGAAAGCTTGTTGCAAGCCAGGCTCCCACTCCCAGCAGACTACCTTGTTGGATTTAATGGATGCCCTCCCCAGCTCAGGCCCTGTTACACAGAAAACTGAGCCCTGGAGTGCAGGAGCCTCTGCTAACCAGACCAACCCCTGGGGTGGAACAGTGGCTCCTTCGAACATTACTGACCCGTGGCCATCGTTTGTTATGGCATTCCTTGCTCCAGGATTTGGGACAGGACGCTGA
Peptide sequence:
MTTSSIRRQMKNIVNNYSEAEIKVREATSNDPWGPSSSLMTEIADLTYNVVAFSEIMSMVWKRLNDHGKNWRHVYKALTLLDYLIKTGSERVAQQCRENIFAIQTLKDFQYIDRDGKDQGINVREKSKQLVALLKDEERLKVERVQALKTKERMAQVATGVGSNQITFGRGSSQPNLSTSYSEQEYGKAGGSPASYHGSTSPRVSSELEQARPQTSGEEELQLQLALAMSREVAEQSSESVQTARGSKEERLRRGDDLRLQMALEESRRDTVKVPKKKEAKACCKPGSHSQQTTLLDLMDALPSSGPVTQKTEPWSAGASANQTNPWGGTVAPSNITDPWPSFVMAFLAPGFGTGR
IRESfinder result
IRESfinder score
0.317530428
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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