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mmu_circEpg5_2008_2
Basic information of circular RNA
PanCircBase ID:
mmu_circEpg5_2008_2
mm39_Chr. Position ID:
chr18|78022338|78054205|+
Gene name:
Epg5
Transcript:
ENSMUST00000044622.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
13
Exon/intron indexes:
17,18,19,20,21,22,23,24,25,26,27,28,29
Exon/intron offsets:
0,1817,2174,3184,4347,5917,7262,9140,12222,16388,18083,30446,31710
Genomic length:
31867
Exon/intron sizes in nt:
14,11,45,19,81,11,12,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr18:78019308-78022338|chr18:78054205-78055915
Splice length:
2008
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CCTTGAGAAGTTCTGTGCAGAAGGCATCCCACTGCTGGGCGTGCTGGTCCAGTCACGGCATCTGAGAGCAGTGGTCCATGCCCTAGATAAGATCCTGCCTGTGTTCTACCCTTACCAGTGCTACCTTCTGAAGAACGAGCAGTTTTTGTCGAACCTTCTCCTCTTCCTACAATTGGACAGCGGTGTTCCCCAGGGGGTCACACAGCAGGTCACCCACAGGGTGGCCCAGCATCTGACAGGAGCCGTCCATGGAGACAATGTGAAACTTCTCAGCAGCATGATCCAGGCACACATATGTGTAAGCACTCAGCCGGATGGAGTGGGTCCTGTGGCTGTGTTGGAGTTCTGGGTCCAGGCTCTCATAAGCCAGCATCTGTGGTACCGAGAACAGCCCATTCTCTTCCTCATGGACCACTTGTGTAAGACAGCGTTTCACCTGATGCAGGAAGATTGTGTGCAAAAATTGCTCTACCAACAGCACAAGAATGCTTTGGGTTACCACTGTGACCGGAGTCTGCTCTCTTCCTTGGTGAACTGGATTGTGGCAGGGAACATCACGCCTTCCTTCGTGGAGGGCTTGTCCACGTCCACACAGGTTTGGTTTGCATGGACTGTGTTGAACATGGAATCCATCTTTGAAGAGGACTCTCAGCTCCGAAGAGTTGTTGAAAGGGAATTGGTTATAAATGCGTTTAGCCCTGACCAAGCTCTAAAGAAAGCCCAGGTCCAGCTGAAGCTGCCCATCGTCCCGTCCCTGCAGCGGCTGTTGATTTATCGCTGGGCCCACCAGGCTCTGGTCACACCTTCTGATCATCCCCTTTTGCCACTCATTTGGCAGAAGTTCTTCCTCCTGTATCTTCATCGCCCAGGACCACAATATGGGTTACCTGTGGATGGTTGTATTGGAAGAAGGTTTTTTCAAAGTCCCTCGCACGTTAATTTGTTGAAAGACATGAAGAGACGCCTGACGGAGGTGGCTGACTTCCACTATGCTGCCAGCAAGGCCCTCCGTGTTCCAGCAGAGGGCAGTGAAGGGACACCAGAAGGGCAGGCTGGCACCCCTGGCTTCCTGACTTCCCCAGAACTGCACAGGGAACTAGTGAGGCTCTTTAATGTATATGTATTATGGCTAGAAGATGAAAATTTTCAGAAAGGAGATACCTATATTCCTTCTTTACCAAAGCACTACGATGTTCACAGGCTAGCAAAAGTGATGCAGAATCAGCAGGACCTGTGGATGGAGTATGTGAATATGGAGCGCATACAGCACGAGTTCCAGGAGACAGTTGCTCTGTGGACACAGGCCAAGCTTGAGTCCCATGCCGCACCCTGCAGCTCTTCAGCACAGCTGGATTTCACGGACCCCTTGCTGGCTAAAGCAAGGGTTCTGAGTAACTTAGAGAAGCACGAGGCTCCACATCCTCCTCTTCTTCTGCACCCCGTGAGGCCTCCCGTGCCACTCATTCCCTCTGCTGCGCTGTTGACACAGAAGGACAGCACCCAGCTCATGTGCACAGACCTGAATCTGCTGCAGCAGCAGGCCAGGAGTGCCACTCTGCGGGAGTCGCAGCAGGTTGCCTTGGATGGCGAGCTGTTGGAGACCATGCCCAAACAGTACGTTAACCGGGAAGATCAGGCCACCCTGCATCTGGAGTGCAGAGGCAGCAGTGGCAAGAAGTGCCAAGGAGCAGCAGTTGTAACGGTCCAGTTTGAAGGCATGAACAAGAATGAAGCCGTAAGCCAGCAGATCCATGTTCTGCAGAAAGAAGTGAGGCAGCTCCAAGCGGAAGCTGCTCAGCCCCCGGCACTGAATGTTGTGGAGGCCGCTGTGCATGCAGAAAACCTGATCACGGCCTTAGTGAACACTTATAAGTTACAGCCTACACCTGGAGTTCAGAAACTTGGCATTAGCCTTTTCTTTACTGTTGTGGACCATGTCAGCGATGAGACCCAGCGCCATCCTCCTACAAGGCAGTTCTTCACTTCATGCATCGAGATCTTGGGACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTGGACCATGTCAGCGATGA
60.11
CTCTCAGATGCCGTGACTGG
60.18
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CATGTCAGCGATGAGACCCA
59.82
CTCTCAGATGCCGTGACTGG
60.18
143
PCR template sequence:
TAGCCTTTTCTTTACTGTTGTGGACCATGTCAGCGATGAGACCCAGCGCCATCCTCCTACAAGGCAGTTCTTCACTTCATGCATCGAGATCTTGGGACAGCCTTGAGAAGTTCTGTGCAGAAGGCATCCCACTGCTGGGCGTGCTGGTCCAGTCACGGCATCTGAGAGCAGTGGTCCATGCCCTAGATAAGATCCTGCCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
25
Interacting miRNAs (#binding sites)
mmu-miR-211-5p(2), mmu-miR-532-5p(2), mmu-miR-9-5p(1), mmu-miR-23b-3p(1), mmu-miR-134-5p(1), mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-181a-5p(1), mmu-miR-103-3p(1), mmu-miR-322-5p(1), mmu-miR-23a-3p(1), mmu-miR-181b-5p(1), mmu-miR-96-5p(1), mmu-miR-324-3p(1), mmu-miR-297a-5p(1), mmu-miR-107-3p(1), mmu-miR-383-5p(1), mmu-miR-342-5p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-466p-5p(1), mmu-miR-369-3p(1), mmu-miR-335-3p(1), mmu-miR-3065-5p(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GATCTTGGGACAGCCTTGAGAAGTTC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEPG5_2008_2_ORF_1
1.188
0.242551066
0.999997473
2082
ORF sequence:
ATGTCAGCGATGAGACCCAGCGCCATCCTCCTACAAGGCAGTTCTTCACTTCATGCATCGAGATCTTGGGACAGCCTTGAGAAGTTCTGTGCAGAAGGCATCCCACTGCTGGGCGTGCTGGTCCAGTCACGGCATCTGAGAGCAGTGGTCCATGCCCTAGATAAGATCCTGCCTGTGTTCTACCCTTACCAGTGCTACCTTCTGAAGAACGAGCAGTTTTTGTCGAACCTTCTCCTCTTCCTACAATTGGACAGCGGTGTTCCCCAGGGGGTCACACAGCAGGTCACCCACAGGGTGGCCCAGCATCTGACAGGAGCCGTCCATGGAGACAATGTGAAACTTCTCAGCAGCATGATCCAGGCACACATATGTGTAAGCACTCAGCCGGATGGAGTGGGTCCTGTGGCTGTGTTGGAGTTCTGGGTCCAGGCTCTCATAAGCCAGCATCTGTGGTACCGAGAACAGCCCATTCTCTTCCTCATGGACCACTTGTGTAAGACAGCGTTTCACCTGATGCAGGAAGATTGTGTGCAAAAATTGCTCTACCAACAGCACAAGAATGCTTTGGGTTACCACTGTGACCGGAGTCTGCTCTCTTCCTTGGTGAACTGGATTGTGGCAGGGAACATCACGCCTTCCTTCGTGGAGGGCTTGTCCACGTCCACACAGGTTTGGTTTGCATGGACTGTGTTGAACATGGAATCCATCTTTGAAGAGGACTCTCAGCTCCGAAGAGTTGTTGAAAGGGAATTGGTTATAAATGCGTTTAGCCCTGACCAAGCTCTAAAGAAAGCCCAGGTCCAGCTGAAGCTGCCCATCGTCCCGTCCCTGCAGCGGCTGTTGATTTATCGCTGGGCCCACCAGGCTCTGGTCACACCTTCTGATCATCCCCTTTTGCCACTCATTTGGCAGAAGTTCTTCCTCCTGTATCTTCATCGCCCAGGACCACAATATGGGTTACCTGTGGATGGTTGTATTGGAAGAAGGTTTTTTCAAAGTCCCTCGCACGTTAATTTGTTGAAAGACATGAAGAGACGCCTGACGGAGGTGGCTGACTTCCACTATGCTGCCAGCAAGGCCCTCCGTGTTCCAGCAGAGGGCAGTGAAGGGACACCAGAAGGGCAGGCTGGCACCCCTGGCTTCCTGACTTCCCCAGAACTGCACAGGGAACTAGTGAGGCTCTTTAATGTATATGTATTATGGCTAGAAGATGAAAATTTTCAGAAAGGAGATACCTATATTCCTTCTTTACCAAAGCACTACGATGTTCACAGGCTAGCAAAAGTGATGCAGAATCAGCAGGACCTGTGGATGGAGTATGTGAATATGGAGCGCATACAGCACGAGTTCCAGGAGACAGTTGCTCTGTGGACACAGGCCAAGCTTGAGTCCCATGCCGCACCCTGCAGCTCTTCAGCACAGCTGGATTTCACGGACCCCTTGCTGGCTAAAGCAAGGGTTCTGAGTAACTTAGAGAAGCACGAGGCTCCACATCCTCCTCTTCTTCTGCACCCCGTGAGGCCTCCCGTGCCACTCATTCCCTCTGCTGCGCTGTTGACACAGAAGGACAGCACCCAGCTCATGTGCACAGACCTGAATCTGCTGCAGCAGCAGGCCAGGAGTGCCACTCTGCGGGAGTCGCAGCAGGTTGCCTTGGATGGCGAGCTGTTGGAGACCATGCCCAAACAGTACGTTAACCGGGAAGATCAGGCCACCCTGCATCTGGAGTGCAGAGGCAGCAGTGGCAAGAAGTGCCAAGGAGCAGCAGTTGTAACGGTCCAGTTTGAAGGCATGAACAAGAATGAAGCCGTAAGCCAGCAGATCCATGTTCTGCAGAAAGAAGTGAGGCAGCTCCAAGCGGAAGCTGCTCAGCCCCCGGCACTGAATGTTGTGGAGGCCGCTGTGCATGCAGAAAACCTGATCACGGCCTTAGTGAACACTTATAAGTTACAGCCTACACCTGGAGTTCAGAAACTTGGCATTAGCCTTTTCTTTACTGTTGTGGACCATGTCAGCGATGAGACCCAGCGCCATCCTCCTACAAGGCAGTTCTTCACTTCATGCATCGAGATCTTGGGACAG
Peptide sequence:
MSAMRPSAILLQGSSSLHASRSWDSLEKFCAEGIPLLGVLVQSRHLRAVVHALDKILPVFYPYQCYLLKNEQFLSNLLLFLQLDSGVPQGVTQQVTHRVAQHLTGAVHGDNVKLLSSMIQAHICVSTQPDGVGPVAVLEFWVQALISQHLWYREQPILFLMDHLCKTAFHLMQEDCVQKLLYQQHKNALGYHCDRSLLSSLVNWIVAGNITPSFVEGLSTSTQVWFAWTVLNMESIFEEDSQLRRVVERELVINAFSPDQALKKAQVQLKLPIVPSLQRLLIYRWAHQALVTPSDHPLLPLIWQKFFLLYLHRPGPQYGLPVDGCIGRRFFQSPSHVNLLKDMKRRLTEVADFHYAASKALRVPAEGSEGTPEGQAGTPGFLTSPELHRELVRLFNVYVLWLEDENFQKGDTYIPSLPKHYDVHRLAKVMQNQQDLWMEYVNMERIQHEFQETVALWTQAKLESHAAPCSSSAQLDFTDPLLAKARVLSNLEKHEAPHPPLLLHPVRPPVPLIPSAALLTQKDSTQLMCTDLNLLQQQARSATLRESQQVALDGELLETMPKQYVNREDQATLHLECRGSSGKKCQGAAVVTVQFEGMNKNEAVSQQIHVLQKEVRQLQAEAAQPPALNVVEAAVHAENLITALVNTYKLQPTPGVQKLGISLFFTVVDHVSDETQRHPPTRQFFTSCIEILGQ
IRESfinder result
IRESfinder score
0.535484409
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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