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mmu_circEpb41l5_805
Basic information of circular RNA
PanCircBase ID:
mmu_circEpb41l5_805
mm39_Chr. Position ID:
chr1|119544130|119570388|-
Gene name:
Epb41l5
Transcript:
ENSMUST00000052404.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,963,1153,3766,4274,5485,7525,17144,26076
Genomic length:
26258
Exon/intron sizes in nt:
89,88,12,15,34,57,94,30,00,000
Flanking exon/introns:
chr1:119543145-119544130|chr1:119570388-119576514
Splice length:
805
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AAATGCTGAGCTTCCTGAGACGAACGCTTGGCCGCAGGTCTATGCGCAAGCATGCTGAAAAGGAGCGGCTCCGAGAGGCACAGCGTGCGGCCACACACATTCCTGCCGCTGGGGATGCCAAGTCCATCATCACATGCAGGGTGTCCCTTCTGGACGGTACAGATGTCAGTGTGGACTTGCCGAAAAAAGCCAAAGGACAGGAGCTGTTTGACCAAATCATGTATCACCTGGACTTGATTGAAAGTGACTATTTTGGTTTGAGATTCATGGATTCAGCCCAAGTAGCCCATTGGTTGGATGGTACAAAAAGCATCAAAAAGCAAGTAAAAATTGGTTCACCCTATTGTCTTCATCTTCGAGTTAAGTTTTATTCCTCGGAACCAAATAATCTTCGTGAGGAGCTAACCCGGTATTTATTTGTTCTTCAGCTAAAACAAGATATTCTCAGTGGGAAGTTAGAGTGTCCCTTTGATACAGCAGTTCAGTTGGCAGCTTATAATCTGCAAGCTGAACTTGGCGACTATGATCTTGCTGAACATAGTCCTGAACTGGTCTCTGAGTTCAGGTTTGTGCCCATCCAGACTGAAGAGATGGAACTGGCTATTTTTGAGAAATGGAAGGAATACAGAGGTCAGACACCAGCACAAGCAGAAACCAATTACCTGAATAAAGCCAAATGGCTAGAAATGTATGGTGTTGATATGCACGTGGTCAAGGCTAGAGATGGCAATGACTATAGTTTGGGACTGACTCCAACAGGAGTCCTTGTTTTTGAAGGAGAGACCAAAATTGGCTTGTTTTTCTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGTCAAGGCTAGAGATGGCA
59.17
CGCTCCTTTTCAGCATGCTT
59.48
163
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGTCAAGGCTAGAGATGGC
59.17
CGCTCCTTTTCAGCATGCTT
59.48
164
PCR template sequence:
ACGTGGTCAAGGCTAGAGATGGCAATGACTATAGTTTGGGACTGACTCCAACAGGAGTCCTTGTTTTTGAAGGAGAGACCAAAATTGGCTTGTTTTTCTGAAATGCTGAGCTTCCTGAGACGAACGCTTGGCCGCAGGTCTATGCGCAAGCATGCTGAAAAGGAGCGGCTCCGAGAGGCACAGCGTGCGGCCACACACAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-324-5p(1), mmu-miR-138-5p(1), mmu-miR-22-3p(1), mmu-miR-125b-1-3p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-466p-5p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
GCTTGTTTTTCTGAAATGCTGAGCTT
siRNA sets
GCATTTCAGAAAAACAAGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEPB41L5_805_ORF_1
1.0902
0.152373564
0.959750204
804
ORF sequence:
ATGCTGAGCTTCCTGAGACGAACGCTTGGCCGCAGGTCTATGCGCAAGCATGCTGAAAAGGAGCGGCTCCGAGAGGCACAGCGTGCGGCCACACACATTCCTGCCGCTGGGGATGCCAAGTCCATCATCACATGCAGGGTGTCCCTTCTGGACGGTACAGATGTCAGTGTGGACTTGCCGAAAAAAGCCAAAGGACAGGAGCTGTTTGACCAAATCATGTATCACCTGGACTTGATTGAAAGTGACTATTTTGGTTTGAGATTCATGGATTCAGCCCAAGTAGCCCATTGGTTGGATGGTACAAAAAGCATCAAAAAGCAAGTAAAAATTGGTTCACCCTATTGTCTTCATCTTCGAGTTAAGTTTTATTCCTCGGAACCAAATAATCTTCGTGAGGAGCTAACCCGGTATTTATTTGTTCTTCAGCTAAAACAAGATATTCTCAGTGGGAAGTTAGAGTGTCCCTTTGATACAGCAGTTCAGTTGGCAGCTTATAATCTGCAAGCTGAACTTGGCGACTATGATCTTGCTGAACATAGTCCTGAACTGGTCTCTGAGTTCAGGTTTGTGCCCATCCAGACTGAAGAGATGGAACTGGCTATTTTTGAGAAATGGAAGGAATACAGAGGTCAGACACCAGCACAAGCAGAAACCAATTACCTGAATAAAGCCAAATGGCTAGAAATGTATGGTGTTGATATGCACGTGGTCAAGGCTAGAGATGGCAATGACTATAGTTTGGGACTGACTCCAACAGGAGTCCTTGTTTTTGAAGGAGAGACCAAAATTGGCTTGTTTTTCTGA
Peptide sequence:
MLSFLRRTLGRRSMRKHAEKERLREAQRAATHIPAAGDAKSIITCRVSLLDGTDVSVDLPKKAKGQELFDQIMYHLDLIESDYFGLRFMDSAQVAHWLDGTKSIKKQVKIGSPYCLHLRVKFYSSEPNNLREELTRYLFVLQLKQDILSGKLECPFDTAVQLAAYNLQAELGDYDLAEHSPELVSEFRFVPIQTEEMELAIFEKWKEYRGQTPAQAETNYLNKAKWLEMYGVDMHVVKARDGNDYSLGLTPTGVLVFEGETKIGLFF
IRESfinder result
IRESfinder score
0.566134519
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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