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mmu_circEp300_2040
Basic information of circular RNA
PanCircBase ID:
mmu_circEp300_2040
mm39_Chr. Position ID:
chr15|81485108|81507726|+
Gene name:
Ep300
Transcript:
ENSMUST00000068387.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
2,3,4,5,6,7,8,9,10,11
Exon/intron offsets:
0,7289,9447,10502,12262,15124,15818,19092,20154,22540
Genomic length:
22618
Exon/intron sizes in nt:
638,177,262,114,246,94,138,118,175,78
Flanking exon/introns:
chr15:81470923-81485108|chr15:81507726-81508725
Splice length:
2040
Other database IDs:
CIRCpedia: MMU_CIRCpedia_30877, circAtlas: mmu-Ep300_0018
circAtlas: hsa-EP300_0032, CIRCpedia: HSA_CIRCpedia_133294
Human Ortholog IDs:
Mature spliced Sequence:
ATTTTGGTTCACTGTTTGACCTGGAACATGACTTACCAGATGAATTAATCAACTCTACAGAATTGGGACTAACCAATGGTGGCGATATCAGTCAGCTTCAGACAAGTCTTGGCATAGTACAAGATGCAGCCTCGAAACATAAACAGCTGTCAGAACTGCTGAGGTCTGGTAGCTCCCCAAACCTCAACATGGGAGTCGGTGGCCCAGGCCAAGCGATGGCCAGCCAGGCCCAACAGAACAGCCCTGGATTAAGTTTGATAAATAGCATGGTCAAAAGCCCAATGGCACAGACAGGCTTGACTTCTCCAAACATGGGGATTGGCAGTAGTGGACCAAATCAGGGTCCTACTCAGTCCCCAGCAGGTATGATGAACAGTCCAGTGAACCAGCCTGCCATGGGAATGAACACAGGGATGAATGCTGGCATGAATCCTGGAATGTTGGCTGCAGGCAATGGACAAGGGATAATGCCCAATCAAGTCATGAACGGTTCCATTGGAGCAGGCCGGGGACGGCCAAACATGCAGTACCCAAATGCAGGCATGGGCAATGCTGGCAGTTTATTGACTGAGCCACTACAGCAGGGCTCTCCTCAGATGGGAGGACAGCCAGGATTGAGAGGCCCCCAACCACTTAAGATGGGAATGATGAACAATCCCAGTCCTTATGGTTCACCATACACTCAGAATTCTGGACAGCAGATTGGAGCAAGTGGCCTTGGTCTCCAAATTCAGACAAAGACTGTTCTACCAAATAACTTATCTCCATTTGCAATGGACAAAAAGGCAGTTCCTGGTGGGGGAATGCCCAGTATGGGCCAGCAGCCTACCCCATCGGTCCAGCAGCCAGGCCTGGTGACTCCAGTTGCCGCAGGAATGGGTTCTGGAGCACACACAGCTGATCCAGAGAAGCGCAAGCTCATCCAGCAGCAGCTTGTTCTCCTTTTACATGCTCACAAGTGCCAGCGCCGGGAGCAAGCTAATGGGGAAGTGAGGCAGTGCAACCTTCCTCACTGTCGTACCATGAAAAATGTCCTAAACCATATGACACATTGCCAGTCAGGCAAATCCTGCCAAGTGGCACATTGTGCATCTTCTCGACAAATCATTTCACACTGGAAAAATTGCACAAGGCATGATTGTCCTGTGTGTCTTCCTCTCAAAAATGCTGGGGATAAGCGAAATCAACAGTCAATTTTGACTGGAGCACCAGTTGGGCTTGGAAACCCTAGCTCTCTAGGAGTGGGGCAGCAGTCCACTCCTAGCCTAAGCACTGTTAGTCAGATTGACCCCAGCTCTATAGAGCGAGCTTACGCTGCTCTTGGACTACCCTATCAAGTAAACCAGATTCCACCACAACCCCAGGTACAAGCAAAGAATCAACAAAGCCAGCCATCTGGACAGTCTCCCCAGGGCATGCGGTCTGTGAACAACATGAGTGCTAGTCCTATGGGTGTAAATGGAGGTGTTGGGGTTCAGACGCCAAATCTTCTTTCTGACTCCATGTTGCATTCAACTATAAATTCTCAAAACCCAATGATGAGTGAAAATGCTGGTGTGGCCTCCCTGGGTCCTTTGCCAACAGCAGCTCAGCCATCTAGTACTGGAATTCGAAAACAGTGGCATGAAGATATTACTCAGGATCTTCGAAACCACCTTGTTCATAAACTTGTTCAAGCCATATTTCCCACTCCGGATCCTGCTGCTTTAAAAGACCGACGGATGGAAAATCTTGTTGCATATGCTCGTAAAGTGGAAGGGGACATGTATGAATCTGCAAACAATAGAGCGGAATACTATCACCTCCTAGCCGAGAAGATCTATAAGATCCAGAAGGAACTAGAAGAGAAACGAAGGACTAGACTACAGAAACAGAACATGCTACCGAATGCTCCTGGCATGGGTCCAGTTCCTATGAATACTGGGTCGAACATGGGGCAGCAACCAACAGGAATGACTACCAATGGTCCTGTACCTGACCCGTCTATGATCCGTGGCAGTGTGCCGAACCACATGATGCCTCGGATGACTCCACAGCCTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GACCCGTCTATGATCCGTGG
59.69
TCGCCACCATTGGTTAGTCC
60.04
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GACCCGTCTATGATCCGTGG
59.69
GCCACCATTGGTTAGTCCCA
59.96
147
PCR template sequence:
CAACCAACAGGAATGACTACCAATGGTCCTGTACCTGACCCGTCTATGATCCGTGGCAGTGTGCCGAACCACATGATGCCTCGGATGACTCCACAGCCTGATTTTGGTTCACTGTTTGACCTGGAACATGACTTACCAGATGAATTAATCAACTCTACAGAATTGGGACTAACCAATGGTGGCGATATCAGTCAGCTTCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
14
Interacting miRNAs (#binding sites)
mmu-miR-370-3p(2), mmu-miR-199a-5p(1), mmu-miR-34a-5p(1), mmu-miR-196a-5p(1), mmu-miR-24-3p(1), mmu-miR-503-5p(1), mmu-miR-136-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-483-5p(1), mmu-miR-182-5p(1), mmu-miR-324-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-207(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ACTCCACAGCCTGATTTTGGTTCACT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEP300_2040_ORF_1
0.9208
0.129207594
1
3891
ORF sequence:
ATGGGAGTCGGTGGCCCAGGCCAAGCGATGGCCAGCCAGGCCCAACAGAACAGCCCTGGATTAAGTTTGATAAATAGCATGGTCAAAAGCCCAATGGCACAGACAGGCTTGACTTCTCCAAACATGGGGATTGGCAGTAGTGGACCAAATCAGGGTCCTACTCAGTCCCCAGCAGGTATGATGAACAGTCCAGTGAACCAGCCTGCCATGGGAATGAACACAGGGATGAATGCTGGCATGAATCCTGGAATGTTGGCTGCAGGCAATGGACAAGGGATAATGCCCAATCAAGTCATGAACGGTTCCATTGGAGCAGGCCGGGGACGGCCAAACATGCAGTACCCAAATGCAGGCATGGGCAATGCTGGCAGTTTATTGACTGAGCCACTACAGCAGGGCTCTCCTCAGATGGGAGGACAGCCAGGATTGAGAGGCCCCCAACCACTTAAGATGGGAATGATGAACAATCCCAGTCCTTATGGTTCACCATACACTCAGAATTCTGGACAGCAGATTGGAGCAAGTGGCCTTGGTCTCCAAATTCAGACAAAGACTGTTCTACCAAATAACTTATCTCCATTTGCAATGGACAAAAAGGCAGTTCCTGGTGGGGGAATGCCCAGTATGGGCCAGCAGCCTACCCCATCGGTCCAGCAGCCAGGCCTGGTGACTCCAGTTGCCGCAGGAATGGGTTCTGGAGCACACACAGCTGATCCAGAGAAGCGCAAGCTCATCCAGCAGCAGCTTGTTCTCCTTTTACATGCTCACAAGTGCCAGCGCCGGGAGCAAGCTAATGGGGAAGTGAGGCAGTGCAACCTTCCTCACTGTCGTACCATGAAAAATGTCCTAAACCATATGACACATTGCCAGTCAGGCAAATCCTGCCAAGTGGCACATTGTGCATCTTCTCGACAAATCATTTCACACTGGAAAAATTGCACAAGGCATGATTGTCCTGTGTGTCTTCCTCTCAAAAATGCTGGGGATAAGCGAAATCAACAGTCAATTTTGACTGGAGCACCAGTTGGGCTTGGAAACCCTAGCTCTCTAGGAGTGGGGCAGCAGTCCACTCCTAGCCTAAGCACTGTTAGTCAGATTGACCCCAGCTCTATAGAGCGAGCTTACGCTGCTCTTGGACTACCCTATCAAGTAAACCAGATTCCACCACAACCCCAGGTACAAGCAAAGAATCAACAAAGCCAGCCATCTGGACAGTCTCCCCAGGGCATGCGGTCTGTGAACAACATGAGTGCTAGTCCTATGGGTGTAAATGGAGGTGTTGGGGTTCAGACGCCAAATCTTCTTTCTGACTCCATGTTGCATTCAACTATAAATTCTCAAAACCCAATGATGAGTGAAAATGCTGGTGTGGCCTCCCTGGGTCCTTTGCCAACAGCAGCTCAGCCATCTAGTACTGGAATTCGAAAACAGTGGCATGAAGATATTACTCAGGATCTTCGAAACCACCTTGTTCATAAACTTGTTCAAGCCATATTTCCCACTCCGGATCCTGCTGCTTTAAAAGACCGACGGATGGAAAATCTTGTTGCATATGCTCGTAAAGTGGAAGGGGACATGTATGAATCTGCAAACAATAGAGCGGAATACTATCACCTCCTAGCCGAGAAGATCTATAAGATCCAGAAGGAACTAGAAGAGAAACGAAGGACTAGACTACAGAAACAGAACATGCTACCGAATGCTCCTGGCATGGGTCCAGTTCCTATGAATACTGGGTCGAACATGGGGCAGCAACCAACAGGAATGACTACCAATGGTCCTGTACCTGACCCGTCTATGATCCGTGGCAGTGTGCCGAACCACATGATGCCTCGGATGACTCCACAGCCTGATTTTGGTTCACTGTTTGACCTGGAACATGACTTACCAGATGAATTAATCAACTCTACAGAATTGGGACTAACCAATGGTGGCGATATCAGTCAGCTTCAGACAAGTCTTGGCATAGTACAAGATGCAGCCTCGAAACATAAACAGCTGTCAGAACTGCTGAGGTCTGGTAGCTCCCCAAACCTCAACATGGGAGTCGGTGGCCCAGGCCAAGCGATGGCCAGCCAGGCCCAACAGAACAGCCCTGGATTAAGTTTGATAAATAGCATGGTCAAAAGCCCAATGGCACAGACAGGCTTGACTTCTCCAAACATGGGGATTGGCAGTAGTGGACCAAATCAGGGTCCTACTCAGTCCCCAGCAGGTATGATGAACAGTCCAGTGAACCAGCCTGCCATGGGAATGAACACAGGGATGAATGCTGGCATGAATCCTGGAATGTTGGCTGCAGGCAATGGACAAGGGATAATGCCCAATCAAGTCATGAACGGTTCCATTGGAGCAGGCCGGGGACGGCCAAACATGCAGTACCCAAATGCAGGCATGGGCAATGCTGGCAGTTTATTGACTGAGCCACTACAGCAGGGCTCTCCTCAGATGGGAGGACAGCCAGGATTGAGAGGCCCCCAACCACTTAAGATGGGAATGATGAACAATCCCAGTCCTTATGGTTCACCATACACTCAGAATTCTGGACAGCAGATTGGAGCAAGTGGCCTTGGTCTCCAAATTCAGACAAAGACTGTTCTACCAAATAACTTATCTCCATTTGCAATGGACAAAAAGGCAGTTCCTGGTGGGGGAATGCCCAGTATGGGCCAGCAGCCTACCCCATCGGTCCAGCAGCCAGGCCTGGTGACTCCAGTTGCCGCAGGAATGGGTTCTGGAGCACACACAGCTGATCCAGAGAAGCGCAAGCTCATCCAGCAGCAGCTTGTTCTCCTTTTACATGCTCACAAGTGCCAGCGCCGGGAGCAAGCTAATGGGGAAGTGAGGCAGTGCAACCTTCCTCACTGTCGTACCATGAAAAATGTCCTAAACCATATGACACATTGCCAGTCAGGCAAATCCTGCCAAGTGGCACATTGTGCATCTTCTCGACAAATCATTTCACACTGGAAAAATTGCACAAGGCATGATTGTCCTGTGTGTCTTCCTCTCAAAAATGCTGGGGATAAGCGAAATCAACAGTCAATTTTGACTGGAGCACCAGTTGGGCTTGGAAACCCTAGCTCTCTAGGAGTGGGGCAGCAGTCCACTCCTAGCCTAAGCACTGTTAGTCAGATTGACCCCAGCTCTATAGAGCGAGCTTACGCTGCTCTTGGACTACCCTATCAAGTAAACCAGATTCCACCACAACCCCAGGTACAAGCAAAGAATCAACAAAGCCAGCCATCTGGACAGTCTCCCCAGGGCATGCGGTCTGTGAACAACATGAGTGCTAGTCCTATGGGTGTAAATGGAGGTGTTGGGGTTCAGACGCCAAATCTTCTTTCTGACTCCATGTTGCATTCAACTATAAATTCTCAAAACCCAATGATGAGTGAAAATGCTGGTGTGGCCTCCCTGGGTCCTTTGCCAACAGCAGCTCAGCCATCTAGTACTGGAATTCGAAAACAGTGGCATGAAGATATTACTCAGGATCTTCGAAACCACCTTGTTCATAAACTTGTTCAAGCCATATTTCCCACTCCGGATCCTGCTGCTTTAAAAGACCGACGGATGGAAAATCTTGTTGCATATGCTCGTAAAGTGGAAGGGGACATGTATGAATCTGCAAACAATAGAGCGGAATACTATCACCTCCTAGCCGAGAAGATCTATAAGATCCAGAAGGAACTAGAAGAGAAACGAAGGACTAGACTACAGAAACAGAACATGCTACCGAATGCTCCTGGCATGGGTCCAGTTCCTATGAATACTGGGTCGAACATGGGGCAGCAACCAACAGGAATGACTACCAATGGTCCTGTACCTGACCCGTCTATGATCCGTGGCAGTGTGCCGAACCACATGATGCCTCGGATGACTCCACAGCCT
Peptide sequence:
MGVGGPGQAMASQAQQNSPGLSLINSMVKSPMAQTGLTSPNMGIGSSGPNQGPTQSPAGMMNSPVNQPAMGMNTGMNAGMNPGMLAAGNGQGIMPNQVMNGSIGAGRGRPNMQYPNAGMGNAGSLLTEPLQQGSPQMGGQPGLRGPQPLKMGMMNNPSPYGSPYTQNSGQQIGASGLGLQIQTKTVLPNNLSPFAMDKKAVPGGGMPSMGQQPTPSVQQPGLVTPVAAGMGSGAHTADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRQCNLPHCRTMKNVLNHMTHCQSGKSCQVAHCASSRQIISHWKNCTRHDCPVCLPLKNAGDKRNQQSILTGAPVGLGNPSSLGVGQQSTPSLSTVSQIDPSSIERAYAALGLPYQVNQIPPQPQVQAKNQQSQPSGQSPQGMRSVNNMSASPMGVNGGVGVQTPNLLSDSMLHSTINSQNPMMSENAGVASLGPLPTAAQPSSTGIRKQWHEDITQDLRNHLVHKLVQAIFPTPDPAALKDRRMENLVAYARKVEGDMYESANNRAEYYHLLAEKIYKIQKELEEKRRTRLQKQNMLPNAPGMGPVPMNTGSNMGQQPTGMTTNGPVPDPSMIRGSVPNHMMPRMTPQPDFGSLFDLEHDLPDELINSTELGLTNGGDISQLQTSLGIVQDAASKHKQLSELLRSGSSPNLNMGVGGPGQAMASQAQQNSPGLSLINSMVKSPMAQTGLTSPNMGIGSSGPNQGPTQSPAGMMNSPVNQPAMGMNTGMNAGMNPGMLAAGNGQGIMPNQVMNGSIGAGRGRPNMQYPNAGMGNAGSLLTEPLQQGSPQMGGQPGLRGPQPLKMGMMNNPSPYGSPYTQNSGQQIGASGLGLQIQTKTVLPNNLSPFAMDKKAVPGGGMPSMGQQPTPSVQQPGLVTPVAAGMGSGAHTADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRQCNLPHCRTMKNVLNHMTHCQSGKSCQVAHCASSRQIISHWKNCTRHDCPVCLPLKNAGDKRNQQSILTGAPVGLGNPSSLGVGQQSTPSLSTVSQIDPSSIERAYAALGLPYQVNQIPPQPQVQAKNQQSQPSGQSPQGMRSVNNMSASPMGVNGGVGVQTPNLLSDSMLHSTINSQNPMMSENAGVASLGPLPTAAQPSSTGIRKQWHEDITQDLRNHLVHKLVQAIFPTPDPAALKDRRMENLVAYARKVEGDMYESANNRAEYYHLLAEKIYKIQKELEEKRRTRLQKQNMLPNAPGMGPVPMNTGSNMGQQPTGMTTNGPVPDPSMIRGSVPNHMMPRMTPQP
IRESfinder result
IRESfinder score
0.492502466
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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