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mmu_circEogt_321
Basic information of circular RNA
PanCircBase ID:
mmu_circEogt_321
mm39_Chr. Position ID:
chr6|97095841|97097765|-
Gene name:
Eogt
Transcript:
ENSMUST00000054344.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
14,13,12,11
Exon/intron offsets:
0,1082,1263,1831
Genomic length:
1924
Exon/intron sizes in nt:
69,87,72,93
Flanking exon/introns:
chr6:97093023-97095841|chr6:97097765-97102480
Splice length:
321
Other database IDs:
circAtlas: mmu-Eogt_0004
CIRCpedia: HSA_CIRCpedia_18652
Human Ortholog IDs:
Mature spliced Sequence:
AGCACTTACGGGTATGGTGACCTCTTCTCCGATACCTGGAAAGCATTTACTGACTATGACGTCATACATTTGAAAACCTATGATTCCAAAAAGGTGTGTTTTAAAGAAGCCGTGTTTTCGTTGCTGCCCCGCATGCGGTACGGGCTGTTCTATAACACGCCTCTGATCTCTGGCTGTCAGAATACAGGATTATTCAGAGCCTTCTCCCAGCATGTGCTGCATAGGCTGAACATCACTCAAGAGGGACCCAAGGATGGCAAAGTTCGAGTTACCATTCTTGCACGCAGCACAGAATACCGGAAAATCCTGAACCAAGACGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGCTGAACATCACTCAAGAGG
60.03
TGCTTTCCAGGTATCGGAGA
58.14
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCTGAACATCACTCAAGAGGGA
60.03
TGCTTTCCAGGTATCGGAGA
58.14
142
PCR template sequence:
TAGGCTGAACATCACTCAAGAGGGACCCAAGGATGGCAAAGTTCGAGTTACCATTCTTGCACGCAGCACAGAATACCGGAAAATCCTGAACCAAGACGAGAGCACTTACGGGTATGGTGACCTCTTCTCCGATACCTGGAAAGCATTTACTGACTATGACGTCATACATTTGAAAACCTATGATTCCAAAAAGGTGTGTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-362-5p(1), mmu-miR-383-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GAACCAAGACGAGAGCACTTACGGGT
siRNA sets
AAGTGCTCTCGTCTTGGTT, ACCCGTAAGTGCTCTCGTC, AGTGCTCTCGTCTTGGTTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEOGT_321_ORF_1
0.9719
0.289659319
0.804375877
510
ORF sequence:
ATGCGGTACGGGCTGTTCTATAACACGCCTCTGATCTCTGGCTGTCAGAATACAGGATTATTCAGAGCCTTCTCCCAGCATGTGCTGCATAGGCTGAACATCACTCAAGAGGGACCCAAGGATGGCAAAGTTCGAGTTACCATTCTTGCACGCAGCACAGAATACCGGAAAATCCTGAACCAAGACGAGAGCACTTACGGGTATGGTGACCTCTTCTCCGATACCTGGAAAGCATTTACTGACTATGACGTCATACATTTGAAAACCTATGATTCCAAAAAGGTGTGTTTTAAAGAAGCCGTGTTTTCGTTGCTGCCCCGCATGCGGTACGGGCTGTTCTATAACACGCCTCTGATCTCTGGCTGTCAGAATACAGGATTATTCAGAGCCTTCTCCCAGCATGTGCTGCATAGGCTGAACATCACTCAAGAGGGACCCAAGGATGGCAAAGTTCGAGTTACCATTCTTGCACGCAGCACAGAATACCGGAAAATCCTGAACCAAGACGAG
Peptide sequence:
MRYGLFYNTPLISGCQNTGLFRAFSQHVLHRLNITQEGPKDGKVRVTILARSTEYRKILNQDESTYGYGDLFSDTWKAFTDYDVIHLKTYDSKKVCFKEAVFSLLPRMRYGLFYNTPLISGCQNTGLFRAFSQHVLHRLNITQEGPKDGKVRVTILARSTEYRKILNQDE
IRESfinder result
IRESfinder score
0.500440947
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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