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mmu_circEntpd5_1263
Basic information of circular RNA
PanCircBase ID:
mmu_circEntpd5_1263
mm39_Chr. Position ID:
chr12|84425953|84443732|-
Gene name:
Entpd5
Transcript:
ENSMUST00000021662.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
12
Exon/intron indexes:
14,13,12,11,10,9,8,7,6,5,4,3
Exon/intron offsets:
0,1141,3052,5039,5968,6617,6958,7558,7800,13226,15444,17499
Genomic length:
17779
Exon/intron sizes in nt:
11,65,71,41,10,26,28,00,00,00,00,00,00,000
Flanking exon/introns:
chr12:84424154-84425953|chr12:84443732-84446113
Splice length:
1263
Other database IDs:
CIRCpedia: MMU_CIRCpedia_225524, circAtlas: mmu-Entpd5_0008
circAtlas: hsa-ENTPD5_0012
Human Ortholog IDs:
Mature spliced Sequence:
CCTCCACAGGAGTGTGAGCAGCACTGCTTCAGCAACAAAGCCTCAGGTCCACATCTTGGGAAGAATATGGCCACTTCCTGGGGGGCTGTCTTCATGCTGATCATAGCCTGCGTTGGCAGCACTGTCTTCTACAGAGAACAGCAGACCTGGTTTGAAGGTGTCTTCTTGTCTTCCATGTGCCCCATTAATGTCAGTGCCGGCACCTTTTATGGAATTATGTTTGATGCGGGCAGCACTGGAACTCGGATTCATGTTTACACTTTTGTGCAGAAAACAGCAGGACAGCTCCCCTTTCTGGAAGGTGAAATTTTTGATTCTGTGAAGCCGGGACTTTCTGCTTTTGTGGATCAGCCCAAACAGGGTGCTGAGACTGTCCAGGAGCTCTTGGAGGTGGCCAAAGACTCGATCCCCAGAAGCCACTGGGAAAGGACCCCGGTGGTTCTGAAAGCAACGGCCGGACTCCGTTTGCTGCCTGAGCAGAAAGCCCAGGCTCTGCTCTTGGAGGTAGAGGAGATCTTCAAGAATTCACCTTTCCTGGTCCCAGATGGCAGCGTTAGCATCATGGATGGGTCCTATGAAGGCATACTAGCCTGGGTTACCGTGAACTTTCTAACAGGTCAGCTGCATGGTCGTGGCCAGGAGACTGTGGGGACCCTTGACCTGGGGGGTGCCTCCACCCAAATCACGTTTCTACCCCAGTTTGAGAAAACCCTGGAACAAACACCTAGGGGCTACCTCACTTCCTTTGAGATGTTTAACAGCACTTTTAAGCTCTATACACATAGTTACTTGGGATTTGGACTGAAAGCTGCAAGACTGGCAACTCTGGGAGCCCTGGAAGCAAAAGGGACTGATGGACATACGTTTCGAAGTGCCTGTTTACCAAGATGGTTGGAAGCAGAGTGGATCTTTGGGGGTGTGAAATACCAGTATGGTGGTAACCAAGAAGGGGAGATGGGCTTTGAACCCTGCTATGCGGAAGTGCTGAGGGTAGTACAGGGGAAACTTCACCAGCCAGAAGAAGTCCGAGGAAGCGCCTTCTACGCTTTCTCTTACTACTACGATCGAGCCGCTGACACACACTTGATCGATTATGAAAAGGGCGGGGTTTTAAAAGTTGAAGATTTTGAAAGAAAAGCCAGAGAAGTGTGTGACAACTTGGGGAGCTTCTCCTCGGGCAGTCCTTTCCTCTGCATGGACCTCACTTACATCACAGCCCTGTTGAAAGATGGTTTTGGCTTTGCCGACGGCACCCTCTTACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCTCTGCATGGACCTCACT
60.25
CCCCAGGAAGTGGCCATATT
59.44
160
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTCTCCTCGGGCAGTCCTTT
60.84
ACCTGAGGCTTTGTTGCTGA
59.82
144
PCR template sequence:
GAGCTTCTCCTCGGGCAGTCCTTTCCTCTGCATGGACCTCACTTACATCACAGCCCTGTTGAAAGATGGTTTTGGCTTTGCCGACGGCACCCTCTTACAGCCTCCACAGGAGTGTGAGCAGCACTGCTTCAGCAACAAAGCCTCAGGTCCACATCTTGGGAAGAATATGGCCACTTCCTGGGGGGCTGTCTTCATGCTGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
15
Interacting miRNAs (#binding sites)
mmu-miR-20b-5p(1), mmu-miR-106a-5p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-106b-5p(1), mmu-miR-298-5p(1), mmu-miR-324-5p(1), mmu-miR-431-5p(1), mmu-miR-212-3p(1), mmu-miR-335-3p(1), mmu-miR-532-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CACCCTCTTACAGCCTCCACAGGAGT
siRNA sets
TGTGGAGGCTGTAAGAGGG, GTGGAGGCTGTAAGAGGGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCENTPD5_1263_ORF_1
0.9355
0.208707368
0.999999707
2460
ORF sequence:
ATGGCCACTTCCTGGGGGGCTGTCTTCATGCTGATCATAGCCTGCGTTGGCAGCACTGTCTTCTACAGAGAACAGCAGACCTGGTTTGAAGGTGTCTTCTTGTCTTCCATGTGCCCCATTAATGTCAGTGCCGGCACCTTTTATGGAATTATGTTTGATGCGGGCAGCACTGGAACTCGGATTCATGTTTACACTTTTGTGCAGAAAACAGCAGGACAGCTCCCCTTTCTGGAAGGTGAAATTTTTGATTCTGTGAAGCCGGGACTTTCTGCTTTTGTGGATCAGCCCAAACAGGGTGCTGAGACTGTCCAGGAGCTCTTGGAGGTGGCCAAAGACTCGATCCCCAGAAGCCACTGGGAAAGGACCCCGGTGGTTCTGAAAGCAACGGCCGGACTCCGTTTGCTGCCTGAGCAGAAAGCCCAGGCTCTGCTCTTGGAGGTAGAGGAGATCTTCAAGAATTCACCTTTCCTGGTCCCAGATGGCAGCGTTAGCATCATGGATGGGTCCTATGAAGGCATACTAGCCTGGGTTACCGTGAACTTTCTAACAGGTCAGCTGCATGGTCGTGGCCAGGAGACTGTGGGGACCCTTGACCTGGGGGGTGCCTCCACCCAAATCACGTTTCTACCCCAGTTTGAGAAAACCCTGGAACAAACACCTAGGGGCTACCTCACTTCCTTTGAGATGTTTAACAGCACTTTTAAGCTCTATACACATAGTTACTTGGGATTTGGACTGAAAGCTGCAAGACTGGCAACTCTGGGAGCCCTGGAAGCAAAAGGGACTGATGGACATACGTTTCGAAGTGCCTGTTTACCAAGATGGTTGGAAGCAGAGTGGATCTTTGGGGGTGTGAAATACCAGTATGGTGGTAACCAAGAAGGGGAGATGGGCTTTGAACCCTGCTATGCGGAAGTGCTGAGGGTAGTACAGGGGAAACTTCACCAGCCAGAAGAAGTCCGAGGAAGCGCCTTCTACGCTTTCTCTTACTACTACGATCGAGCCGCTGACACACACTTGATCGATTATGAAAAGGGCGGGGTTTTAAAAGTTGAAGATTTTGAAAGAAAAGCCAGAGAAGTGTGTGACAACTTGGGGAGCTTCTCCTCGGGCAGTCCTTTCCTCTGCATGGACCTCACTTACATCACAGCCCTGTTGAAAGATGGTTTTGGCTTTGCCGACGGCACCCTCTTACAGCCTCCACAGGAGTGTGAGCAGCACTGCTTCAGCAACAAAGCCTCAGGTCCACATCTTGGGAAGAATATGGCCACTTCCTGGGGGGCTGTCTTCATGCTGATCATAGCCTGCGTTGGCAGCACTGTCTTCTACAGAGAACAGCAGACCTGGTTTGAAGGTGTCTTCTTGTCTTCCATGTGCCCCATTAATGTCAGTGCCGGCACCTTTTATGGAATTATGTTTGATGCGGGCAGCACTGGAACTCGGATTCATGTTTACACTTTTGTGCAGAAAACAGCAGGACAGCTCCCCTTTCTGGAAGGTGAAATTTTTGATTCTGTGAAGCCGGGACTTTCTGCTTTTGTGGATCAGCCCAAACAGGGTGCTGAGACTGTCCAGGAGCTCTTGGAGGTGGCCAAAGACTCGATCCCCAGAAGCCACTGGGAAAGGACCCCGGTGGTTCTGAAAGCAACGGCCGGACTCCGTTTGCTGCCTGAGCAGAAAGCCCAGGCTCTGCTCTTGGAGGTAGAGGAGATCTTCAAGAATTCACCTTTCCTGGTCCCAGATGGCAGCGTTAGCATCATGGATGGGTCCTATGAAGGCATACTAGCCTGGGTTACCGTGAACTTTCTAACAGGTCAGCTGCATGGTCGTGGCCAGGAGACTGTGGGGACCCTTGACCTGGGGGGTGCCTCCACCCAAATCACGTTTCTACCCCAGTTTGAGAAAACCCTGGAACAAACACCTAGGGGCTACCTCACTTCCTTTGAGATGTTTAACAGCACTTTTAAGCTCTATACACATAGTTACTTGGGATTTGGACTGAAAGCTGCAAGACTGGCAACTCTGGGAGCCCTGGAAGCAAAAGGGACTGATGGACATACGTTTCGAAGTGCCTGTTTACCAAGATGGTTGGAAGCAGAGTGGATCTTTGGGGGTGTGAAATACCAGTATGGTGGTAACCAAGAAGGGGAGATGGGCTTTGAACCCTGCTATGCGGAAGTGCTGAGGGTAGTACAGGGGAAACTTCACCAGCCAGAAGAAGTCCGAGGAAGCGCCTTCTACGCTTTCTCTTACTACTACGATCGAGCCGCTGACACACACTTGATCGATTATGAAAAGGGCGGGGTTTTAAAAGTTGAAGATTTTGAAAGAAAAGCCAGAGAAGTGTGTGACAACTTGGGGAGCTTCTCCTCGGGCAGTCCTTTCCTCTGCATGGACCTCACTTACATCACAGCCCTGTTGAAAGATGGTTTTGGCTTTGCCGACGGCACCCTCTTACAG
Peptide sequence:
MATSWGAVFMLIIACVGSTVFYREQQTWFEGVFLSSMCPINVSAGTFYGIMFDAGSTGTRIHVYTFVQKTAGQLPFLEGEIFDSVKPGLSAFVDQPKQGAETVQELLEVAKDSIPRSHWERTPVVLKATAGLRLLPEQKAQALLLEVEEIFKNSPFLVPDGSVSIMDGSYEGILAWVTVNFLTGQLHGRGQETVGTLDLGGASTQITFLPQFEKTLEQTPRGYLTSFEMFNSTFKLYTHSYLGFGLKAARLATLGALEAKGTDGHTFRSACLPRWLEAEWIFGGVKYQYGGNQEGEMGFEPCYAEVLRVVQGKLHQPEEVRGSAFYAFSYYYDRAADTHLIDYEKGGVLKVEDFERKAREVCDNLGSFSSGSPFLCMDLTYITALLKDGFGFADGTLLQPPQECEQHCFSNKASGPHLGKNMATSWGAVFMLIIACVGSTVFYREQQTWFEGVFLSSMCPINVSAGTFYGIMFDAGSTGTRIHVYTFVQKTAGQLPFLEGEIFDSVKPGLSAFVDQPKQGAETVQELLEVAKDSIPRSHWERTPVVLKATAGLRLLPEQKAQALLLEVEEIFKNSPFLVPDGSVSIMDGSYEGILAWVTVNFLTGQLHGRGQETVGTLDLGGASTQITFLPQFEKTLEQTPRGYLTSFEMFNSTFKLYTHSYLGFGLKAARLATLGALEAKGTDGHTFRSACLPRWLEAEWIFGGVKYQYGGNQEGEMGFEPCYAEVLRVVQGKLHQPEEVRGSAFYAFSYYYDRAADTHLIDYEKGGVLKVEDFERKAREVCDNLGSFSSGSPFLCMDLTYITALLKDGFGFADGTLLQ
IRESfinder result
IRESfinder score
0.625723647
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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