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mmu_circEnox2_498
Basic information of circular RNA
PanCircBase ID:
mmu_circEnox2_498
mm39_Chr. Position ID:
chrX|48151019|48169628|-
Gene name:
Enox2
Transcript:
ENSMUST00000114914.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
6,5,4
Exon/intron offsets:
0,7492,18474
Genomic length:
18609
Exon/intron sizes in nt:
20,71,56,135
Flanking exon/introns:
chrX:48145445-48151019|chrX:48169628-48257517
Splice length:
498
Other database IDs:
circBase: mmu_circ_0016293, CIRCpedia: MMU_CIRCpedia_12172, circAtlas: mmu-Enox2_0004
circBase: hsa_circ_0139783, circAtlas: hsa-ENOX2_0003, CIRCpedia: HSA_CIRCpedia_65213
Human Ortholog IDs:
Mature spliced Sequence:
CTGATAATACTAGAACTCTGAATGTAGATTCTGCTGCAATGACGCTGCCTGTGTCTGATCCAGCTGCATGGGCCACAGCAATGAATAATCTTGGAATGGCTCCACTGGGAATTGCTGGACAACCAATTTTACCTGACTTCGATCCTGCCCTTGGGATGATGACTGGAATACCACCAATAACTCCCATGATGCCGGGTTTGGGCATAGTCCCGCCACCGATTCCTCCAGATATGCCGGTAGCAAAGGAGATCATACACTGCAAAAGCTGCACGCTCTTCCCTCCCAACCCAAATCTTCCACCACCTGCAACACGAGAAAGGCCACCAGGCTGTAAGACAGTGTTTGTGGGTGGCCTGCCTGAAAATGGGACAGAGCAGATCATTGTGGAAGTGTTTGAACAGTGTGGAGAGATTATTGCTATCCGGAAGAGCAAAAAGAACTTCTGTCACATTCGCTTTGCTGAGGAATACATGGTGGACAAAGCCCTCTATCTGTCTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTCACATTCGCTTTGCTGAGG
60.14
ATTCATTGCTGTGGCCCATG
59.17
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCACATTCGCTTTGCTGAG
59.47
ATTCATTGCTGTGGCCCATG
59.17
141
PCR template sequence:
CAGTGTGGAGAGATTATTGCTATCCGGAAGAGCAAAAAGAACTTCTGTCACATTCGCTTTGCTGAGGAATACATGGTGGACAAAGCCCTCTATCTGTCTGCTGATAATACTAGAACTCTGAATGTAGATTCTGCTGCAATGACGCTGCCTGTGTCTGATCCAGCTGCATGGGCCACAGCAATGAATAATCTTGGAATGGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-34b-5p(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTCTATCTGTCTGCTGATAATACTAG
siRNA sets
TAGTATTATCAGCAGACAG, CTAGTATTATCAGCAGACA, AGTATTATCAGCAGACAGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCENOX2_498_ORF_1
1.2154
0.222063876
0.991900181
957
ORF sequence:
ATGACGCTGCCTGTGTCTGATCCAGCTGCATGGGCCACAGCAATGAATAATCTTGGAATGGCTCCACTGGGAATTGCTGGACAACCAATTTTACCTGACTTCGATCCTGCCCTTGGGATGATGACTGGAATACCACCAATAACTCCCATGATGCCGGGTTTGGGCATAGTCCCGCCACCGATTCCTCCAGATATGCCGGTAGCAAAGGAGATCATACACTGCAAAAGCTGCACGCTCTTCCCTCCCAACCCAAATCTTCCACCACCTGCAACACGAGAAAGGCCACCAGGCTGTAAGACAGTGTTTGTGGGTGGCCTGCCTGAAAATGGGACAGAGCAGATCATTGTGGAAGTGTTTGAACAGTGTGGAGAGATTATTGCTATCCGGAAGAGCAAAAAGAACTTCTGTCACATTCGCTTTGCTGAGGAATACATGGTGGACAAAGCCCTCTATCTGTCTGCTGATAATACTAGAACTCTGAATGTAGATTCTGCTGCAATGACGCTGCCTGTGTCTGATCCAGCTGCATGGGCCACAGCAATGAATAATCTTGGAATGGCTCCACTGGGAATTGCTGGACAACCAATTTTACCTGACTTCGATCCTGCCCTTGGGATGATGACTGGAATACCACCAATAACTCCCATGATGCCGGGTTTGGGCATAGTCCCGCCACCGATTCCTCCAGATATGCCGGTAGCAAAGGAGATCATACACTGCAAAAGCTGCACGCTCTTCCCTCCCAACCCAAATCTTCCACCACCTGCAACACGAGAAAGGCCACCAGGCTGTAAGACAGTGTTTGTGGGTGGCCTGCCTGAAAATGGGACAGAGCAGATCATTGTGGAAGTGTTTGAACAGTGTGGAGAGATTATTGCTATCCGGAAGAGCAAAAAGAACTTCTGTCACATTCGCTTTGCTGAGGAATACATGGTGGACAAAGCCCTCTATCTGTCT
Peptide sequence:
MTLPVSDPAAWATAMNNLGMAPLGIAGQPILPDFDPALGMMTGIPPITPMMPGLGIVPPPIPPDMPVAKEIIHCKSCTLFPPNPNLPPPATRERPPGCKTVFVGGLPENGTEQIIVEVFEQCGEIIAIRKSKKNFCHIRFAEEYMVDKALYLSADNTRTLNVDSAAMTLPVSDPAAWATAMNNLGMAPLGIAGQPILPDFDPALGMMTGIPPITPMMPGLGIVPPPIPPDMPVAKEIIHCKSCTLFPPNPNLPPPATRERPPGCKTVFVGGLPENGTEQIIVEVFEQCGEIIAIRKSKKNFCHIRFAEEYMVDKALYLS
IRESfinder result
IRESfinder score
0.425019123
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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