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mmu_circEnoph1_696
Basic information of circular RNA
PanCircBase ID:
mmu_circEnoph1_696
mm39_Chr. Position ID:
chr5|100206998|100215800|+
Gene name:
Enoph1
Transcript:
ENSMUST00000031268.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
2,3,4,5,6
Exon/intron offsets:
0,1834,3003,4619,8659
Genomic length:
8802
Exon/intron sizes in nt:
102,194,133,124,143
Flanking exon/introns:
chr5:100188161-100206998|chr5:100215800-100216154
Splice length:
696
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GATGTTTTATTTCCGTACATCAAAGAAAATGTCAAGGAGTACCTGCAGACCCACTGGGAGGAGGAGGAGTGTCAGCAGGACGTCAGCCTGCTGAGGAAACAGGCCGAGGAAGATGCACATCTGGACGGGGCTGTTCCGATACCCGTGGCTTCTGGCAGTGATCTGCAGCAGATGATCCAGGCTGTGGTGGACAACGTGTACTGGCAGATGTCCCATGACCGAAAGACCACAGCGCTCAAACAGCTGCAAGGCCACATGTGGAAGGCAGCTTTCACGGCTGGCCGCATGAAGGCAGAGTTCTTTGCAGATGTGGTTCCTGCAGTCAGAAGGTGGAGAGAGGCTGGGATGAAGGTATACATCTATTCTTCAGGGAGCGTGGAGGCACAGAAGCTGCTCTTCGGCCATTCCACAGAGGGAGACATTCTTGAGCTCATCGACGGTCACTTTGACACCAAGATTGGACACAAAGTGGACAGTGAGAGTTACCGGAAGATTGCTGATAGCATCGGTTGTTCAACCAACAACATCCTGTTTCTCACGGATGTCACCGTAGAGGCCAGTGCTGCTGAGGAGGCGGACGTGCACGTGGCTGTGGTGGTGCGACCTGGCAACGCGGGGCTCACTGACGACGAGAAGACATACTACAACCTCATCACGTCCTTCAGCGAGCTCTACCTGCCGTCCACCTAGAGCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGCTCACTGACGACGAGAAG
60.46
CTGACGTCCTGCTGACACTC
60.39
166
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCTCACTGACGACGAGAAGA
59.48
CTGACGTCCTGCTGACACTC
60.39
165
PCR template sequence:
GGTGCGACCTGGCAACGCGGGGCTCACTGACGACGAGAAGACATACTACAACCTCATCACGTCCTTCAGCGAGCTCTACCTGCCGTCCACCTAGAGCCAGGATGTTTTATTTCCGTACATCAAAGAAAATGTCAAGGAGTACCTGCAGACCCACTGGGAGGAGGAGGAGTGTCAGCAGGACGTCAGCCTGCTGAGGAAAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-205-5p(1), mmu-miR-670-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CACCTAGAGCCAGGATGTTTTATTTC
siRNA sets
AAATAAAACATCCTGGCTC, AATAAAACATCCTGGCTCT, TAAAACATCCTGGCTCTAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.293644363
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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