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mmu_circEnoph1_257
Basic information of circular RNA
PanCircBase ID:
mmu_circEnoph1_257
mm39_Chr. Position ID:
chr5|100210001|100211741|+
Gene name:
Enoph1
Transcript:
ENSMUST00000169390.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
4,5
Exon/intron offsets:
0,1616
Genomic length:
1740
Exon/intron sizes in nt:
1,33,124
Flanking exon/introns:
chr5:100209026-100210001|chr5:100211741-100215657
Splice length:
257
Other database IDs:
CIRCpedia: MMU_CIRCpedia_19
circBase: hsa_circ_0003451, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_49274
Human Ortholog IDs:
Mature spliced Sequence:
GTTCTTTGCAGATGTGGTTCCTGCAGTCAGAAGGTGGAGAGAGGCTGGGATGAAGGTATACATCTATTCTTCAGGGAGCGTGGAGGCACAGAAGCTGCTCTTCGGCCATTCCACAGAGGGAGACATTCTTGAGCTCATCGACGGTCACTTTGACACCAAGATTGGACACAAAGTGGACAGTGAGAGTTACCGGAAGATTGCTGATAGCATCGGTTGTTCAACCAACAACATCCTGTTTCTCACGGATGTCACCGTAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTGGACAGTGAGAGTTACCGG
60.07
ACCTTCATCCCAGCCTCTCT
59.96
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGACAGTGAGAGTTACCGGA
60.2
ACCTTCATCCCAGCCTCTCT
59.96
141
PCR template sequence:
AAGATTGGACACAAAGTGGACAGTGAGAGTTACCGGAAGATTGCTGATAGCATCGGTTGTTCAACCAACAACATCCTGTTTCTCACGGATGTCACCGTAGGTTCTTTGCAGATGTGGTTCCTGCAGTCAGAAGGTGGAGAGAGGCTGGGATGAAGGTATACATCTATTCTTCAGGGAGCGTGGAGGCACAGAAGCTGCTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-205-5p(1), mmu-miR-670-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GATGTCACCGTAGGTTCTTTGCAGAT
siRNA sets
ATCTGCAAAGAACCTACGG, AAGAACCTACGGTGACATC, AAAGAACCTACGGTGACAT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCENOPH1_257_ORF_1
1.0181
0.34631821
0.484396574
261
ORF sequence:
ATGAAGGTATACATCTATTCTTCAGGGAGCGTGGAGGCACAGAAGCTGCTCTTCGGCCATTCCACAGAGGGAGACATTCTTGAGCTCATCGACGGTCACTTTGACACCAAGATTGGACACAAAGTGGACAGTGAGAGTTACCGGAAGATTGCTGATAGCATCGGTTGTTCAACCAACAACATCCTGTTTCTCACGGATGTCACCGTAGGTTCTTTGCAGATGTGGTTCCTGCAGTCAGAAGGTGGAGAGAGGCTGGGATGA
Peptide sequence:
MKVYIYSSGSVEAQKLLFGHSTEGDILELIDGHFDTKIGHKVDSESYRKIADSIGCSTNNILFLTDVTVGSLQMWFLQSEGGERLG
IRESfinder result
IRESfinder score
0.515455063
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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