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mmu_circEnah_253
Basic information of circular RNA
PanCircBase ID:
mmu_circEnah_253
mm39_Chr. Position ID:
chr1|181735862|181745880|-
Gene name:
Enah
Transcript:
ENSMUST00000193703.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
12,11,10,9
Exon/intron offsets:
0,5009,8901,9960
Genomic length:
10018
Exon/intron sizes in nt:
79,67,49,58
Flanking exon/introns:
chr1:181734009-181735862|chr1:181745880-181746002
Splice length:
253
Other database IDs:
circAtlas: mmu-Enah_0011
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
GAGAAGAATTGCTGAGAAGGGATCAACAATAGAAACAGAACAAAAGGAAGACAGAAATGAAGATGCAGAGCCTATAACTGCTAAGGCCCCATCAACAAGTACACCTGAACCAACCAGAAAACCTTGGGAAAGAACAAACACAATGAACGGCAGTAAGTCACCTGTCATCTCCAGACCCAAATCCACACCTTCATCACAGCCAAGTGCCAATGGAGTCCAGACAGAAGGCCTTGACTATGACAGGCTGAAGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTGCCAATGGAGTCCAGACA
59.96
GTTGATGGGGCCTTAGCAGT
60.03
146
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCCACACCTTCATCACAGCC
59.96
GTTGATGGGGCCTTAGCAGT
60.03
168
PCR template sequence:
TAAGTCACCTGTCATCTCCAGACCCAAATCCACACCTTCATCACAGCCAAGTGCCAATGGAGTCCAGACAGAAGGCCTTGACTATGACAGGCTGAAGCAGGAGAAGAATTGCTGAGAAGGGATCAACAATAGAAACAGAACAAAAGGAAGACAGAAATGAAGATGCAGAGCCTATAACTGCTAAGGCCCCATCAACAAGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-136-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CAGGCTGAAGCAGGAGAAGAATTGCT
siRNA sets
TTCTTCTCCTGCTTCAGCC, ATTCTTCTCCTGCTTCAGC, AATTCTTCTCCTGCTTCAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCENAH_253_ORF_1
0.5655
0.265594629
0.09901599
126
ORF sequence:
ATGAACGGCAGTAAGTCACCTGTCATCTCCAGACCCAAATCCACACCTTCATCACAGCCAAGTGCCAATGGAGTCCAGACAGAAGGCCTTGACTATGACAGGCTGAAGCAGGAGAAGAATTGCTGA
Peptide sequence:
MNGSKSPVISRPKSTPSSQPSANGVQTEGLDYDRLKQEKNC
IRESfinder result
IRESfinder score
0.423216387
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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