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mmu_circEnah_166
Basic information of circular RNA
PanCircBase ID:
mmu_circEnah_166
mm39_Chr. Position ID:
chr1|181789318|181789484|-
Gene name:
Enah
Transcript:
ENSMUST00000193703.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
2
Exon/intron offsets:
0
Genomic length:
166
Exon/intron sizes in nt:
166
Flanking exon/introns:
chr1:181784136-181789318|chr1:181789484-181847094
Splice length:
166
Other database IDs:
CIRCpedia: MMU_CIRCpedia_213828, circAtlas: mmu-Enah_0041
circBase: hsa_circ_0016663, CIRCpedia: HSA_CIRCpedia_110659
Human Ortholog IDs:
Mature spliced Sequence:
TGAACAGAGTATCTGTCAGGCAAGAGCTGCTGTGATGGTCTATGATGATGCCAATAAGAAGTGGGTGCCAGCTGGTGGCTCAACTGGGTTCAGCAGAGTACATATATATCACCATACAGGCAACAACACATTCAGAGTTGTGGGCAGAAAGATTCAAGACCATCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CACCATACAGGCAACAACACA
59.05
CAGCTGGCACCCACTTCTTA
59.96
131
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCACCATACAGGCAACAACAC
58.77
CAGCTGGCACCCACTTCTTA
59.96
132
PCR template sequence:
CTGGGTTCAGCAGAGTACATATATATCACCATACAGGCAACAACACATTCAGAGTTGTGGGCAGAAAGATTCAAGACCATCAGTGAACAGAGTATCTGTCAGGCAAGAGCTGCTGTGATGGTCTATGATGATGCCAATAAGAAGTGGGTGCCAGCTGGTGGCTCAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-1198-5p(1), mmu-miR-673-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TCAAGACCATCAGTGAACAGAGTATC
siRNA sets
TACTCTGTTCACTGATGGT, TGTTCACTGATGGTCTTGA, ATACTCTGTTCACTGATGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCENAH_166_ORF_1
0.7403
0.074798347
0.187180269
291
ORF sequence:
ATGATGATGCCAATAAGAAGTGGGTGCCAGCTGGTGGCTCAACTGGGTTCAGCAGAGTACATATATATCACCATACAGGCAACAACACATTCAGAGTTGTGGGCAGAAAGATTCAAGACCATCAGTGAACAGAGTATCTGTCAGGCAAGAGCTGCTGTGATGGTCTATGATGATGCCAATAAGAAGTGGGTGCCAGCTGGTGGCTCAACTGGGTTCAGCAGAGTACATATATATCACCATACAGGCAACAACACATTCAGAGTTGTGGGCAGAAAGATTCAAGACCATCAG
Peptide sequence:
MMMPIRSGCQLVAQLGSAEYIYITIQATTHSELWAERFKTISEQSICQARAAVMVYDDANKKWVPAGGSTGFSRVHIYHHTGNNTFRVVGRKIQDHQ
IRESfinder result
IRESfinder score
0.374649506
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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