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mmu_circEml5_1869
Basic information of circular RNA
PanCircBase ID:
mmu_circEml5_1869
mm39_Chr. Position ID:
chr12|98782193|98818960|-
Gene name:
Eml5
Transcript:
ENSMUST00000065716.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
14
Exon/intron indexes:
29,28,27,26,25,24,23,22,21,20,19,18,17,16
Exon/intron offsets:
0,1885,8699,9444,11399,12772,14925,15137,21308,25616,26737,28019,35447,36673
Genomic length:
36767
Exon/intron sizes in nt:
130,79,127,202,177,89,132,153,142,128,112,203,101,94
Flanking exon/introns:
chr12:98776876-98782193|chr12:98818960-98822182
Splice length:
1869
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CTGATGGGAAACGCCTGGCTTCAGTTGGAATAGATGACAGCCACACCATTGTATTGTGGGACTGGAAGAAAGGGGAGAAACTTTCAGTGACAAGAGGCAGTAAAGATAAGATTTTTGTTGTGAAAATGAACCCCTATGTGCCTGATAAACTAATTACAGCTGGGATTAAACACATGAAATTTTGGCGTAGAGCAGGGGGAGGATTAATTGGAAAAAAAGGCTACGTAGGCACTCTGGGGAAAAACGACACAATGATGTGTGCAGTGTACGGATGGACTGAAGAGATGGCTTTTTCTGGAACATCCACAGGAGATGTGTGCATCTGGAGAGATGTTTTTCTTGTAAAAACAGTTAAAGCCCATGATGGGCCCGTGTTCAGTATGCATGCATTGGAGAAAGGATTTGTAACTGGAGGAAAAGATGGTATGGTAGCCCTTTGGGATGATTCCTTTGAAAGATGCCTCAAGACCTATGCTATAAAAAGAGCTGATTTAGCCCCAGGATCTAAAGGTCTGCTCTTGGAAGATAACCCATCTATACGTGCCATATCATTAGGGCATGGTCATATTTTGGTTGGCACAAAGAATGGTGAGATATTAGAAGTGGATAAGAGTGGCCCAATCACTCTTCTGGTCCAGGGACACATGGAAGGAGAGGTGTGGGGTTTGGCCACACATCCTTACCTGCCCATCTGTGCTACTGTAAGTGATGATAAGACCTTAAGAATATGGGATCTCTCTCCTAGTCATTGTATGTTGGCAGTCCGGAAACTGAAAAAGGGGGGCCGCTGCTGCTGCTTTTCCCCTGATGGAAAAGCTTTGGCTGTGGGTCTCAACGACGGAAGCTTCTTAATGGCCAATGCTGACACTCTGGAGGATCTTGTGTCCTTTCACCACAGAAAAGATATTATTTCAGACATCCGGTTTTCACCTGGTTCTGGGAAATACCTAGCTGTGGCATCCCATGACAGCTTCGTTGATATATACAACGTGACGAGTAGTAAACGAGTGGGGGTTTGCAAAGGAGCAACCAGCTACATCACCCATATCGACTGGGACAGCAGAGGAAAGCTTTTACAAGTCAACACTGGTGCTAAAGAACAGCTATTCTTTGAAGCTCCCAGAGGAAAAAGACAAACCATCCCCAGTGTGGAGGTGGAAAAAATTAGTTGGGCAACATGGACAAGTGTTCTTGGTTTATGCTGTGAGGGAATTTGGCCGGTGATTGGAGAAGTCACAGAAGTAACCGCCTCTTGCCTCACCAGTGACAAAATGGTCCTAGCCACAGGGGATGACTTGGGATTCGTGAAGCTGTTCAGATACCCAGCTAAAGGAAAATTTGGAAAGTTTAAGAAGTATGTGGCTCACAGCACACATGTCACAAATGTTCGCTGGACTTATGATGACAGCATGCTGGTTACCCTGGGAGGAGCAGATATGTCCTTAATGGTATGGACAAATGAAGTGGAGAGCCATCGAGAAAAGAAGTACTGTGACAGTGAAGAGTCTGATATAGATTCTGAAGAAGATGGAGGCTATGACAGCGATGTTACAAGAGAGAATGAAATTAGCTATACCATCAGAGCCTTATCAACAAATATTCGCCCAATGTTTGGAGTCAAGCCTCATTTGCAACAGAAAGAGCCATCAGTTGATGAAAGGCCTCCAGTGAGTAGGGCCCCACCACAGCCAGAGAAACTTCAGTCAAACAATGTTGGCAAGAAGAAGAGACCTATAGAGGACCTTGTGTTGGAGCTTGCTTTTGGCTATCGGGGCAGAGACTGCAGGAACAATGTGCACTATTTAAATGATGGTGATGATATAATTTATCACACTGCCTCTATTGGAATTCTACACAATGTTGCCACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACTGCAGGAACAATGTGCAC
59.33
CCCCTTTCTTCCAGTCCCAC
59.96
165
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACTGCAGGAACAATGTGCAC
59.33
TCCCCTTTCTTCCAGTCCCA
60.1
166
PCR template sequence:
CGGGGCAGAGACTGCAGGAACAATGTGCACTATTTAAATGATGGTGATGATATAATTTATCACACTGCCTCTATTGGAATTCTACACAATGTTGCCACAGCTGATGGGAAACGCCTGGCTTCAGTTGGAATAGATGACAGCCACACCATTGTATTGTGGGACTGGAAGAAAGGGGAGAAACTTTCAGTGACAAGAGGCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
16
Interacting miRNAs (#binding sites)
mmu-miR-1198-5p(2), mmu-miR-27b-3p(1), mmu-miR-199a-5p(1), mmu-miR-135a-5p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-326-3p(1), mmu-miR-183-5p(1), mmu-miR-758-3p(1), mmu-miR-34b-5p(1), mmu-miR-383-5p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-466p-5p(1), mmu-miR-188-3p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AATGTTGCCACAGCTGATGGGAAACG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEML5_1869_ORF_1
0.8664
0.099831932
1
3612
ORF sequence:
ATGAACCCCTATGTGCCTGATAAACTAATTACAGCTGGGATTAAACACATGAAATTTTGGCGTAGAGCAGGGGGAGGATTAATTGGAAAAAAAGGCTACGTAGGCACTCTGGGGAAAAACGACACAATGATGTGTGCAGTGTACGGATGGACTGAAGAGATGGCTTTTTCTGGAACATCCACAGGAGATGTGTGCATCTGGAGAGATGTTTTTCTTGTAAAAACAGTTAAAGCCCATGATGGGCCCGTGTTCAGTATGCATGCATTGGAGAAAGGATTTGTAACTGGAGGAAAAGATGGTATGGTAGCCCTTTGGGATGATTCCTTTGAAAGATGCCTCAAGACCTATGCTATAAAAAGAGCTGATTTAGCCCCAGGATCTAAAGGTCTGCTCTTGGAAGATAACCCATCTATACGTGCCATATCATTAGGGCATGGTCATATTTTGGTTGGCACAAAGAATGGTGAGATATTAGAAGTGGATAAGAGTGGCCCAATCACTCTTCTGGTCCAGGGACACATGGAAGGAGAGGTGTGGGGTTTGGCCACACATCCTTACCTGCCCATCTGTGCTACTGTAAGTGATGATAAGACCTTAAGAATATGGGATCTCTCTCCTAGTCATTGTATGTTGGCAGTCCGGAAACTGAAAAAGGGGGGCCGCTGCTGCTGCTTTTCCCCTGATGGAAAAGCTTTGGCTGTGGGTCTCAACGACGGAAGCTTCTTAATGGCCAATGCTGACACTCTGGAGGATCTTGTGTCCTTTCACCACAGAAAAGATATTATTTCAGACATCCGGTTTTCACCTGGTTCTGGGAAATACCTAGCTGTGGCATCCCATGACAGCTTCGTTGATATATACAACGTGACGAGTAGTAAACGAGTGGGGGTTTGCAAAGGAGCAACCAGCTACATCACCCATATCGACTGGGACAGCAGAGGAAAGCTTTTACAAGTCAACACTGGTGCTAAAGAACAGCTATTCTTTGAAGCTCCCAGAGGAAAAAGACAAACCATCCCCAGTGTGGAGGTGGAAAAAATTAGTTGGGCAACATGGACAAGTGTTCTTGGTTTATGCTGTGAGGGAATTTGGCCGGTGATTGGAGAAGTCACAGAAGTAACCGCCTCTTGCCTCACCAGTGACAAAATGGTCCTAGCCACAGGGGATGACTTGGGATTCGTGAAGCTGTTCAGATACCCAGCTAAAGGAAAATTTGGAAAGTTTAAGAAGTATGTGGCTCACAGCACACATGTCACAAATGTTCGCTGGACTTATGATGACAGCATGCTGGTTACCCTGGGAGGAGCAGATATGTCCTTAATGGTATGGACAAATGAAGTGGAGAGCCATCGAGAAAAGAAGTACTGTGACAGTGAAGAGTCTGATATAGATTCTGAAGAAGATGGAGGCTATGACAGCGATGTTACAAGAGAGAATGAAATTAGCTATACCATCAGAGCCTTATCAACAAATATTCGCCCAATGTTTGGAGTCAAGCCTCATTTGCAACAGAAAGAGCCATCAGTTGATGAAAGGCCTCCAGTGAGTAGGGCCCCACCACAGCCAGAGAAACTTCAGTCAAACAATGTTGGCAAGAAGAAGAGACCTATAGAGGACCTTGTGTTGGAGCTTGCTTTTGGCTATCGGGGCAGAGACTGCAGGAACAATGTGCACTATTTAAATGATGGTGATGATATAATTTATCACACTGCCTCTATTGGAATTCTACACAATGTTGCCACAGCTGATGGGAAACGCCTGGCTTCAGTTGGAATAGATGACAGCCACACCATTGTATTGTGGGACTGGAAGAAAGGGGAGAAACTTTCAGTGACAAGAGGCAGTAAAGATAAGATTTTTGTTGTGAAAATGAACCCCTATGTGCCTGATAAACTAATTACAGCTGGGATTAAACACATGAAATTTTGGCGTAGAGCAGGGGGAGGATTAATTGGAAAAAAAGGCTACGTAGGCACTCTGGGGAAAAACGACACAATGATGTGTGCAGTGTACGGATGGACTGAAGAGATGGCTTTTTCTGGAACATCCACAGGAGATGTGTGCATCTGGAGAGATGTTTTTCTTGTAAAAACAGTTAAAGCCCATGATGGGCCCGTGTTCAGTATGCATGCATTGGAGAAAGGATTTGTAACTGGAGGAAAAGATGGTATGGTAGCCCTTTGGGATGATTCCTTTGAAAGATGCCTCAAGACCTATGCTATAAAAAGAGCTGATTTAGCCCCAGGATCTAAAGGTCTGCTCTTGGAAGATAACCCATCTATACGTGCCATATCATTAGGGCATGGTCATATTTTGGTTGGCACAAAGAATGGTGAGATATTAGAAGTGGATAAGAGTGGCCCAATCACTCTTCTGGTCCAGGGACACATGGAAGGAGAGGTGTGGGGTTTGGCCACACATCCTTACCTGCCCATCTGTGCTACTGTAAGTGATGATAAGACCTTAAGAATATGGGATCTCTCTCCTAGTCATTGTATGTTGGCAGTCCGGAAACTGAAAAAGGGGGGCCGCTGCTGCTGCTTTTCCCCTGATGGAAAAGCTTTGGCTGTGGGTCTCAACGACGGAAGCTTCTTAATGGCCAATGCTGACACTCTGGAGGATCTTGTGTCCTTTCACCACAGAAAAGATATTATTTCAGACATCCGGTTTTCACCTGGTTCTGGGAAATACCTAGCTGTGGCATCCCATGACAGCTTCGTTGATATATACAACGTGACGAGTAGTAAACGAGTGGGGGTTTGCAAAGGAGCAACCAGCTACATCACCCATATCGACTGGGACAGCAGAGGAAAGCTTTTACAAGTCAACACTGGTGCTAAAGAACAGCTATTCTTTGAAGCTCCCAGAGGAAAAAGACAAACCATCCCCAGTGTGGAGGTGGAAAAAATTAGTTGGGCAACATGGACAAGTGTTCTTGGTTTATGCTGTGAGGGAATTTGGCCGGTGATTGGAGAAGTCACAGAAGTAACCGCCTCTTGCCTCACCAGTGACAAAATGGTCCTAGCCACAGGGGATGACTTGGGATTCGTGAAGCTGTTCAGATACCCAGCTAAAGGAAAATTTGGAAAGTTTAAGAAGTATGTGGCTCACAGCACACATGTCACAAATGTTCGCTGGACTTATGATGACAGCATGCTGGTTACCCTGGGAGGAGCAGATATGTCCTTAATGGTATGGACAAATGAAGTGGAGAGCCATCGAGAAAAGAAGTACTGTGACAGTGAAGAGTCTGATATAGATTCTGAAGAAGATGGAGGCTATGACAGCGATGTTACAAGAGAGAATGAAATTAGCTATACCATCAGAGCCTTATCAACAAATATTCGCCCAATGTTTGGAGTCAAGCCTCATTTGCAACAGAAAGAGCCATCAGTTGATGAAAGGCCTCCAGTGAGTAGGGCCCCACCACAGCCAGAGAAACTTCAGTCAAACAATGTTGGCAAGAAGAAGAGACCTATAGAGGACCTTGTGTTGGAGCTTGCTTTTGGCTATCGGGGCAGAGACTGCAGGAACAATGTGCACTATTTAAATGATGGTGATGATATAATTTATCACACTGCCTCTATTGGAATTCTACACAATGTTGCCACA
Peptide sequence:
MNPYVPDKLITAGIKHMKFWRRAGGGLIGKKGYVGTLGKNDTMMCAVYGWTEEMAFSGTSTGDVCIWRDVFLVKTVKAHDGPVFSMHALEKGFVTGGKDGMVALWDDSFERCLKTYAIKRADLAPGSKGLLLEDNPSIRAISLGHGHILVGTKNGEILEVDKSGPITLLVQGHMEGEVWGLATHPYLPICATVSDDKTLRIWDLSPSHCMLAVRKLKKGGRCCCFSPDGKALAVGLNDGSFLMANADTLEDLVSFHHRKDIISDIRFSPGSGKYLAVASHDSFVDIYNVTSSKRVGVCKGATSYITHIDWDSRGKLLQVNTGAKEQLFFEAPRGKRQTIPSVEVEKISWATWTSVLGLCCEGIWPVIGEVTEVTASCLTSDKMVLATGDDLGFVKLFRYPAKGKFGKFKKYVAHSTHVTNVRWTYDDSMLVTLGGADMSLMVWTNEVESHREKKYCDSEESDIDSEEDGGYDSDVTRENEISYTIRALSTNIRPMFGVKPHLQQKEPSVDERPPVSRAPPQPEKLQSNNVGKKKRPIEDLVLELAFGYRGRDCRNNVHYLNDGDDIIYHTASIGILHNVATADGKRLASVGIDDSHTIVLWDWKKGEKLSVTRGSKDKIFVVKMNPYVPDKLITAGIKHMKFWRRAGGGLIGKKGYVGTLGKNDTMMCAVYGWTEEMAFSGTSTGDVCIWRDVFLVKTVKAHDGPVFSMHALEKGFVTGGKDGMVALWDDSFERCLKTYAIKRADLAPGSKGLLLEDNPSIRAISLGHGHILVGTKNGEILEVDKSGPITLLVQGHMEGEVWGLATHPYLPICATVSDDKTLRIWDLSPSHCMLAVRKLKKGGRCCCFSPDGKALAVGLNDGSFLMANADTLEDLVSFHHRKDIISDIRFSPGSGKYLAVASHDSFVDIYNVTSSKRVGVCKGATSYITHIDWDSRGKLLQVNTGAKEQLFFEAPRGKRQTIPSVEVEKISWATWTSVLGLCCEGIWPVIGEVTEVTASCLTSDKMVLATGDDLGFVKLFRYPAKGKFGKFKKYVAHSTHVTNVRWTYDDSMLVTLGGADMSLMVWTNEVESHREKKYCDSEESDIDSEEDGGYDSDVTRENEISYTIRALSTNIRPMFGVKPHLQQKEPSVDERPPVSRAPPQPEKLQSNNVGKKKRPIEDLVLELAFGYRGRDCRNNVHYLNDGDDIIYHTASIGILHNVAT
IRESfinder result
IRESfinder score
0.515016009
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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