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mmu_circElovl5_510
Basic information of circular RNA
PanCircBase ID:
mmu_circElovl5_510
mm39_Chr. Position ID:
chr9|77883066|77888890|+
Gene name:
Elovl5
Transcript:
ENSMUST00000034904.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
4,5,6,7
Exon/intron offsets:
0,963,4040,5689
Genomic length:
5824
Exon/intron sizes in nt:
78,17,21,25,135
Flanking exon/introns:
chr9:77868335-77883066|chr9:77888890-77889946
Splice length:
510
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TTGGTGACAGGTGTGTGGGAAGGCAAATACAACTTTTTCTGCCAGGGAACACGCAGCGCGGGAGAATCCGATATGAAGATCATCCGCGTCCTCTGGTGGTACTACTTCTCCAAACTCATCGAATTCATGGACACCTTTTTCTTCATCCTTCGCAAGAACAACCACCAGATCACCGTGCTCCATGTCTACCACCACGCTACCATGCTCAACATCTGGTGGTTTGTGATGAACTGGGTTCCCTGCGGCCATTCATATTTTGGTGCGACACTCAACAGCTTCATCCATGTCCTCATGTACTCGTACTATGGTCTGTCCTCCATCCCGTCCATGCGTCCCTACCTCTGGTGGAAAAAGTACATCACTCAAGGGCAGCTGGTCCAGTTTGTGCTGACAATCATCCAGACGACCTGCGGGGTCTTCTGGCCATGCTCCTTCCCTCTCGGGTGGCTGTTCTTCCAGATTGGATACATGATTTCCCTGATTGCTCTCTTCACAAACTTCTACATTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGGTGGCTGTTCTTCCAGAT
59.67
GAGGACGCGGATGATCTTCA
59.61
162
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGGTGGCTGTTCTTCCAGAT
59.67
CAGAGGACGCGGATGATCTT
59.61
164
PCR template sequence:
CGGGGTCTTCTGGCCATGCTCCTTCCCTCTCGGGTGGCTGTTCTTCCAGATTGGATACATGATTTCCCTGATTGCTCTCTTCACAAACTTCTACATTCAGTTGGTGACAGGTGTGTGGGAAGGCAAATACAACTTTTTCTGCCAGGGAACACGCAGCGCGGGAGAATCCGATATGAAGATCATCCGCGTCCTCTGGTGGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-let-7a-5p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1), mmu-miR-298-5p(1), mmu-let-7d-5p(1), mmu-miR-433-5p(1), mmu-miR-22-3p(1), mmu-miR-350-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CTTCTACATTCAGTTGGTGACAGGTG
siRNA sets
TGTCACCAACTGAATGTAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCELOVL5_510_ORF_1
1.1571
0.298555526
0.992942539
948
ORF sequence:
ATGAAGATCATCCGCGTCCTCTGGTGGTACTACTTCTCCAAACTCATCGAATTCATGGACACCTTTTTCTTCATCCTTCGCAAGAACAACCACCAGATCACCGTGCTCCATGTCTACCACCACGCTACCATGCTCAACATCTGGTGGTTTGTGATGAACTGGGTTCCCTGCGGCCATTCATATTTTGGTGCGACACTCAACAGCTTCATCCATGTCCTCATGTACTCGTACTATGGTCTGTCCTCCATCCCGTCCATGCGTCCCTACCTCTGGTGGAAAAAGTACATCACTCAAGGGCAGCTGGTCCAGTTTGTGCTGACAATCATCCAGACGACCTGCGGGGTCTTCTGGCCATGCTCCTTCCCTCTCGGGTGGCTGTTCTTCCAGATTGGATACATGATTTCCCTGATTGCTCTCTTCACAAACTTCTACATTCAGTTGGTGACAGGTGTGTGGGAAGGCAAATACAACTTTTTCTGCCAGGGAACACGCAGCGCGGGAGAATCCGATATGAAGATCATCCGCGTCCTCTGGTGGTACTACTTCTCCAAACTCATCGAATTCATGGACACCTTTTTCTTCATCCTTCGCAAGAACAACCACCAGATCACCGTGCTCCATGTCTACCACCACGCTACCATGCTCAACATCTGGTGGTTTGTGATGAACTGGGTTCCCTGCGGCCATTCATATTTTGGTGCGACACTCAACAGCTTCATCCATGTCCTCATGTACTCGTACTATGGTCTGTCCTCCATCCCGTCCATGCGTCCCTACCTCTGGTGGAAAAAGTACATCACTCAAGGGCAGCTGGTCCAGTTTGTGCTGACAATCATCCAGACGACCTGCGGGGTCTTCTGGCCATGCTCCTTCCCTCTCGGGTGGCTGTTCTTCCAGATTGGATACATGATTTCCCTGATTGCTCTCTTCACAAACTTCTACATTCAG
Peptide sequence:
MKIIRVLWWYYFSKLIEFMDTFFFILRKNNHQITVLHVYHHATMLNIWWFVMNWVPCGHSYFGATLNSFIHVLMYSYYGLSSIPSMRPYLWWKKYITQGQLVQFVLTIIQTTCGVFWPCSFPLGWLFFQIGYMISLIALFTNFYIQLVTGVWEGKYNFFCQGTRSAGESDMKIIRVLWWYYFSKLIEFMDTFFFILRKNNHQITVLHVYHHATMLNIWWFVMNWVPCGHSYFGATLNSFIHVLMYSYYGLSSIPSMRPYLWWKKYITQGQLVQFVLTIIQTTCGVFWPCSFPLGWLFFQIGYMISLIALFTNFYIQ
IRESfinder result
IRESfinder score
0.624985798
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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