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mmu_circElmo2_609
Basic information of circular RNA
PanCircBase ID:
mmu_circElmo2_609
mm39_Chr. Position ID:
chr2|165138760|165140662|-
Gene name:
Elmo2
Transcript:
ENSMUST00000103091.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
15,14,13,12,11
Exon/intron offsets:
0,506,700,1368,1779
Genomic length:
1902
Exon/intron sizes in nt:
13,71,09,10,51,35,123
Flanking exon/introns:
chr2:165137546-165138760|chr2:165140662-165143860
Splice length:
609
Other database IDs:
CIRCpedia: MMU_CIRCpedia_11512, circAtlas: mmu-Elmo2_0007
Human Ortholog IDs:
Mature spliced Sequence:
CATGTGATCAGAGGGAATCGTCCAATCAAAACAGAGATGGCCCATCAGCTGTATGTCCTTCAGGTCTTGACCTTTAACCTTCTGGAAGAAAGAATGATGACCAAGATGGATCCCAATGACCAGGCTCAGAGAGACATTATATTTGAACTGAGGAGGATTGCCTTCGACGCAGAGTCTGACCCCAGCAACGTCCCCGGGAGTGGGACTGAAAAGCGCAAGGCCATGTATACCAAGGACTATAAAATGCTGGGCTTCACCAACCATATCAACCCAGCCTTGGACTTCACCCAGACTCCTCCTGGAATGCTGGCGCTGGACAACATGCTGTACCTGGCTAAAGTCCACCAGGACACCTACATCCGGATCGTGCTGGAGAACAGCAGTCGGGAGGACAAACACGAGTGTCCGTTCGGCCGCAGTGCCATCGAGCTCACCAAGATGCTCTGTGAGATCCTGCAGGTCGGGGAGCTCCCTAATGAAGGGCGCAATGACTACCACCCCATGTTCTTCACCCACGACCGAGCCTTCGAGGAACTCTTCGGGATCTGCATCCAGCTGCTGAACAAGACCTGGAAGGAGATGAGGGCGACAGCCGAGGATTTCAACAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGAACTCTTCGGGATCTGC
59.17
AAGGACATACAGCTGATGGGC
60.13
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGAACTCTTCGGGATCTGCA
59.46
TGAAGGACATACAGCTGATGGG
59.83
141
PCR template sequence:
CACCCACGACCGAGCCTTCGAGGAACTCTTCGGGATCTGCATCCAGCTGCTGAACAAGACCTGGAAGGAGATGAGGGCGACAGCCGAGGATTTCAACAAGCATGTGATCAGAGGGAATCGTCCAATCAAAACAGAGATGGCCCATCAGCTGTATGTCCTTCAGGTCTTGACCTTTAACCTTCTGGAAGAAAGAATGATGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-103-3p(1), mmu-miR-183-5p(1), mmu-miR-107-3p(1), mmu-miR-5101(1)
siRNAs for circular RNA silencing
Junction sequence
GGATTTCAACAAGCATGTGATCAGAG
siRNA sets
CACATGCTTGTTGAAATCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCELMO2_609_ORF_1
1.3174
0.557795856
0.999660808
1182
ORF sequence:
ATGGCCCATCAGCTGTATGTCCTTCAGGTCTTGACCTTTAACCTTCTGGAAGAAAGAATGATGACCAAGATGGATCCCAATGACCAGGCTCAGAGAGACATTATATTTGAACTGAGGAGGATTGCCTTCGACGCAGAGTCTGACCCCAGCAACGTCCCCGGGAGTGGGACTGAAAAGCGCAAGGCCATGTATACCAAGGACTATAAAATGCTGGGCTTCACCAACCATATCAACCCAGCCTTGGACTTCACCCAGACTCCTCCTGGAATGCTGGCGCTGGACAACATGCTGTACCTGGCTAAAGTCCACCAGGACACCTACATCCGGATCGTGCTGGAGAACAGCAGTCGGGAGGACAAACACGAGTGTCCGTTCGGCCGCAGTGCCATCGAGCTCACCAAGATGCTCTGTGAGATCCTGCAGGTCGGGGAGCTCCCTAATGAAGGGCGCAATGACTACCACCCCATGTTCTTCACCCACGACCGAGCCTTCGAGGAACTCTTCGGGATCTGCATCCAGCTGCTGAACAAGACCTGGAAGGAGATGAGGGCGACAGCCGAGGATTTCAACAAGCATGTGATCAGAGGGAATCGTCCAATCAAAACAGAGATGGCCCATCAGCTGTATGTCCTTCAGGTCTTGACCTTTAACCTTCTGGAAGAAAGAATGATGACCAAGATGGATCCCAATGACCAGGCTCAGAGAGACATTATATTTGAACTGAGGAGGATTGCCTTCGACGCAGAGTCTGACCCCAGCAACGTCCCCGGGAGTGGGACTGAAAAGCGCAAGGCCATGTATACCAAGGACTATAAAATGCTGGGCTTCACCAACCATATCAACCCAGCCTTGGACTTCACCCAGACTCCTCCTGGAATGCTGGCGCTGGACAACATGCTGTACCTGGCTAAAGTCCACCAGGACACCTACATCCGGATCGTGCTGGAGAACAGCAGTCGGGAGGACAAACACGAGTGTCCGTTCGGCCGCAGTGCCATCGAGCTCACCAAGATGCTCTGTGAGATCCTGCAGGTCGGGGAGCTCCCTAATGAAGGGCGCAATGACTACCACCCCATGTTCTTCACCCACGACCGAGCCTTCGAGGAACTCTTCGGGATCTGCATCCAGCTGCTGAACAAGACCTGGAAGGAGATGAGGGCGACAGCCGAGGATTTCAACAAG
Peptide sequence:
MAHQLYVLQVLTFNLLEERMMTKMDPNDQAQRDIIFELRRIAFDAESDPSNVPGSGTEKRKAMYTKDYKMLGFTNHINPALDFTQTPPGMLALDNMLYLAKVHQDTYIRIVLENSSREDKHECPFGRSAIELTKMLCEILQVGELPNEGRNDYHPMFFTHDRAFEELFGICIQLLNKTWKEMRATAEDFNKHVIRGNRPIKTEMAHQLYVLQVLTFNLLEERMMTKMDPNDQAQRDIIFELRRIAFDAESDPSNVPGSGTEKRKAMYTKDYKMLGFTNHINPALDFTQTPPGMLALDNMLYLAKVHQDTYIRIVLENSSREDKHECPFGRSAIELTKMLCEILQVGELPNEGRNDYHPMFFTHDRAFEELFGICIQLLNKTWKEMRATAEDFNK
IRESfinder result
IRESfinder score
0.340063597
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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