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mmu_circElmo2_1077
Basic information of circular RNA
PanCircBase ID:
mmu_circElmo2_1077
mm39_Chr. Position ID:
chr2|165136160|165140662|-
Gene name:
Elmo2
Transcript:
ENSMUST00000103091.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
19,18,17,16,15,14,13,12,11
Exon/intron offsets:
0,773,1003,1222,2600,3106,3300,3968,4379
Genomic length:
4502
Exon/intron sizes in nt:
8,31,08,11,31,64,13,71,00,00,00,00,000
Flanking exon/introns:
chr2:165134102-165136160|chr2:165140662-165143860
Splice length:
1077
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CATGTGATCAGAGGGAATCGTCCAATCAAAACAGAGATGGCCCATCAGCTGTATGTCCTTCAGGTCTTGACCTTTAACCTTCTGGAAGAAAGAATGATGACCAAGATGGATCCCAATGACCAGGCTCAGAGAGACATTATATTTGAACTGAGGAGGATTGCCTTCGACGCAGAGTCTGACCCCAGCAACGTCCCCGGGAGTGGGACTGAAAAGCGCAAGGCCATGTATACCAAGGACTATAAAATGCTGGGCTTCACCAACCATATCAACCCAGCCTTGGACTTCACCCAGACTCCTCCTGGAATGCTGGCGCTGGACAACATGCTGTACCTGGCTAAAGTCCACCAGGACACCTACATCCGGATCGTGCTGGAGAACAGCAGTCGGGAGGACAAACACGAGTGTCCGTTCGGCCGCAGTGCCATCGAGCTCACCAAGATGCTCTGTGAGATCCTGCAGGTCGGGGAGCTCCCTAATGAAGGGCGCAATGACTACCACCCCATGTTCTTCACCCACGACCGAGCCTTCGAGGAACTCTTCGGGATCTGCATCCAGCTGCTGAACAAGACCTGGAAGGAGATGAGGGCGACAGCCGAGGATTTCAACAAGGTTATGCAAGTTGTCCGAGAGCAGATCACCCGGGCTCTGCCCTCTAAACCCAACTCTTTGGATCAGTTCAAGAGTAAACTTCGTAGCCTGAGCTACTCAGAAATTCTGCGGTTGCGCCAGTCTGAGAGGATGAGCCAGGATGATTTCCAGTCCCCACCAATTGTGGAGCTTCGAGAGAAGATACAGCCTGAGATCCTGGAGCTGATCAAGCAACAGCGCCTCAACCGGCTATGCGAGGGCAGCAGCTTCCGGAAAATCGGGAACCGTCGTCGGCAAGAGAGGTTCTGGCACTGCCGCTTGGCACTGAACCACAAGGTTTTGCATTACGGTGACTTGGATGACAACCCTCAAGGGGAGGTGACATTCGAATCCCTGCAGGAGAAAATTCCTGTTGCAGATATTAAGGCCATTGTTACTGGAAAAGACTGTCCTCACATGAAAGAGAAGAGTGCCCTGAAACAGAACAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCCTGCAGGAGAAAATTCC
57.49
GCTGATGGGCCATCTCTGTT
60.11
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCCCTGCAGGAGAAAATTCC
57.49
GACATACAGCTGATGGGCCA
59.82
156
PCR template sequence:
ATCCCTGCAGGAGAAAATTCCTGTTGCAGATATTAAGGCCATTGTTACTGGAAAAGACTGTCCTCACATGAAAGAGAAGAGTGCCCTGAAACAGAACAAGCATGTGATCAGAGGGAATCGTCCAATCAAAACAGAGATGGCCCATCAGCTGTATGTCCTTCAGGTCTTGACCTTTAACCTTCTGGAAGAAAGAATGATGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-103-3p(1), mmu-miR-34a-5p(1), mmu-miR-149-5p(1), mmu-miR-183-5p(1), mmu-miR-541-5p(1), mmu-miR-107-3p(1), mmu-miR-224-5p(1), mmu-miR-802-5p(1), mmu-miR-5101(1)
siRNAs for circular RNA silencing
Junction sequence
GAAACAGAACAAGCATGTGATCAGAG
siRNA sets
TGATCACATGCTTGTTCTG, GATCACATGCTTGTTCTGT, ATCACATGCTTGTTCTGTT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCELMO2_1077_ORF_1
1.3114
0.513266085
0.99999951
2118
ORF sequence:
ATGGCCCATCAGCTGTATGTCCTTCAGGTCTTGACCTTTAACCTTCTGGAAGAAAGAATGATGACCAAGATGGATCCCAATGACCAGGCTCAGAGAGACATTATATTTGAACTGAGGAGGATTGCCTTCGACGCAGAGTCTGACCCCAGCAACGTCCCCGGGAGTGGGACTGAAAAGCGCAAGGCCATGTATACCAAGGACTATAAAATGCTGGGCTTCACCAACCATATCAACCCAGCCTTGGACTTCACCCAGACTCCTCCTGGAATGCTGGCGCTGGACAACATGCTGTACCTGGCTAAAGTCCACCAGGACACCTACATCCGGATCGTGCTGGAGAACAGCAGTCGGGAGGACAAACACGAGTGTCCGTTCGGCCGCAGTGCCATCGAGCTCACCAAGATGCTCTGTGAGATCCTGCAGGTCGGGGAGCTCCCTAATGAAGGGCGCAATGACTACCACCCCATGTTCTTCACCCACGACCGAGCCTTCGAGGAACTCTTCGGGATCTGCATCCAGCTGCTGAACAAGACCTGGAAGGAGATGAGGGCGACAGCCGAGGATTTCAACAAGGTTATGCAAGTTGTCCGAGAGCAGATCACCCGGGCTCTGCCCTCTAAACCCAACTCTTTGGATCAGTTCAAGAGTAAACTTCGTAGCCTGAGCTACTCAGAAATTCTGCGGTTGCGCCAGTCTGAGAGGATGAGCCAGGATGATTTCCAGTCCCCACCAATTGTGGAGCTTCGAGAGAAGATACAGCCTGAGATCCTGGAGCTGATCAAGCAACAGCGCCTCAACCGGCTATGCGAGGGCAGCAGCTTCCGGAAAATCGGGAACCGTCGTCGGCAAGAGAGGTTCTGGCACTGCCGCTTGGCACTGAACCACAAGGTTTTGCATTACGGTGACTTGGATGACAACCCTCAAGGGGAGGTGACATTCGAATCCCTGCAGGAGAAAATTCCTGTTGCAGATATTAAGGCCATTGTTACTGGAAAAGACTGTCCTCACATGAAAGAGAAGAGTGCCCTGAAACAGAACAAGCATGTGATCAGAGGGAATCGTCCAATCAAAACAGAGATGGCCCATCAGCTGTATGTCCTTCAGGTCTTGACCTTTAACCTTCTGGAAGAAAGAATGATGACCAAGATGGATCCCAATGACCAGGCTCAGAGAGACATTATATTTGAACTGAGGAGGATTGCCTTCGACGCAGAGTCTGACCCCAGCAACGTCCCCGGGAGTGGGACTGAAAAGCGCAAGGCCATGTATACCAAGGACTATAAAATGCTGGGCTTCACCAACCATATCAACCCAGCCTTGGACTTCACCCAGACTCCTCCTGGAATGCTGGCGCTGGACAACATGCTGTACCTGGCTAAAGTCCACCAGGACACCTACATCCGGATCGTGCTGGAGAACAGCAGTCGGGAGGACAAACACGAGTGTCCGTTCGGCCGCAGTGCCATCGAGCTCACCAAGATGCTCTGTGAGATCCTGCAGGTCGGGGAGCTCCCTAATGAAGGGCGCAATGACTACCACCCCATGTTCTTCACCCACGACCGAGCCTTCGAGGAACTCTTCGGGATCTGCATCCAGCTGCTGAACAAGACCTGGAAGGAGATGAGGGCGACAGCCGAGGATTTCAACAAGGTTATGCAAGTTGTCCGAGAGCAGATCACCCGGGCTCTGCCCTCTAAACCCAACTCTTTGGATCAGTTCAAGAGTAAACTTCGTAGCCTGAGCTACTCAGAAATTCTGCGGTTGCGCCAGTCTGAGAGGATGAGCCAGGATGATTTCCAGTCCCCACCAATTGTGGAGCTTCGAGAGAAGATACAGCCTGAGATCCTGGAGCTGATCAAGCAACAGCGCCTCAACCGGCTATGCGAGGGCAGCAGCTTCCGGAAAATCGGGAACCGTCGTCGGCAAGAGAGGTTCTGGCACTGCCGCTTGGCACTGAACCACAAGGTTTTGCATTACGGTGACTTGGATGACAACCCTCAAGGGGAGGTGACATTCGAATCCCTGCAGGAGAAAATTCCTGTTGCAGATATTAAGGCCATTGTTACTGGAAAAGACTGTCCTCACATGAAAGAGAAGAGTGCCCTGAAACAGAACAAG
Peptide sequence:
MAHQLYVLQVLTFNLLEERMMTKMDPNDQAQRDIIFELRRIAFDAESDPSNVPGSGTEKRKAMYTKDYKMLGFTNHINPALDFTQTPPGMLALDNMLYLAKVHQDTYIRIVLENSSREDKHECPFGRSAIELTKMLCEILQVGELPNEGRNDYHPMFFTHDRAFEELFGICIQLLNKTWKEMRATAEDFNKVMQVVREQITRALPSKPNSLDQFKSKLRSLSYSEILRLRQSERMSQDDFQSPPIVELREKIQPEILELIKQQRLNRLCEGSSFRKIGNRRRQERFWHCRLALNHKVLHYGDLDDNPQGEVTFESLQEKIPVADIKAIVTGKDCPHMKEKSALKQNKHVIRGNRPIKTEMAHQLYVLQVLTFNLLEERMMTKMDPNDQAQRDIIFELRRIAFDAESDPSNVPGSGTEKRKAMYTKDYKMLGFTNHINPALDFTQTPPGMLALDNMLYLAKVHQDTYIRIVLENSSREDKHECPFGRSAIELTKMLCEILQVGELPNEGRNDYHPMFFTHDRAFEELFGICIQLLNKTWKEMRATAEDFNKVMQVVREQITRALPSKPNSLDQFKSKLRSLSYSEILRLRQSERMSQDDFQSPPIVELREKIQPEILELIKQQRLNRLCEGSSFRKIGNRRRQERFWHCRLALNHKVLHYGDLDDNPQGEVTFESLQEKIPVADIKAIVTGKDCPHMKEKSALKQNK
IRESfinder result
IRESfinder score
0.346285911
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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