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mmu_circElapor2_348
Basic information of circular RNA
PanCircBase ID:
mmu_circElapor2_348
mm39_Chr. Position ID:
chr5|9470616|9472390|+
Gene name:
Elapor2
Transcript:
ENSMUST00000155764.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
6,7,8
Exon/intron offsets:
0,888,1685
Genomic length:
1774
Exon/intron sizes in nt:
106,153,89
Flanking exon/introns:
chr5:9468071-9470616|chr5:9472390-9477689
Splice length:
348
Other database IDs:
CIRCpedia: HSA_CIRCpedia_360422
Human Ortholog IDs:
Mature spliced Sequence:
GTAATGCTGAAATCAGGCACAAATATTCTGTACTGGAGAACGACAGGCATCCTTATGGGTTCTAAGGCAGTCAAGCCCGTGCTAGTAAAGAATATCACCATCGAAGGGGTGGCATACACCTCAGAGTGCTTTCCATGTAAGCCAGGCACTTTCAGCAACAAACCAGGCTCATTTAACTGCCAGATGTGTCCCAGAAACACCTATTCTGAGAAGGGTGCTAAAGAATGTATTAGGTGTAAGGAGGACTCCCAGTTTTCAGAGGAAGGAGCCAGTGAGTGTGTGGATCGCCCACCCTGCACTACCAAAGACTATTTCCAGATCCATACCCCCTGTGACGAAGAAGGGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAGGAGCCAGTGAGTGTGTG
59.89
AAGGATGCCTGTCGTTCTCC
59.75
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGAGGAAGGAGCCAGTGA
59.88
GATGCCTGTCGTTCTCCAGT
59.75
144
PCR template sequence:
CCAGTTTTCAGAGGAAGGAGCCAGTGAGTGTGTGGATCGCCCACCCTGCACTACCAAAGACTATTTCCAGATCCATACCCCCTGTGACGAAGAAGGGAAGGTAATGCTGAAATCAGGCACAAATATTCTGTACTGGAGAACGACAGGCATCCTTATGGGTTCTAAGGCAGTCAAGCCCGTGCTAGTAAAGAATATCACCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-miR-149-5p(1), mmu-miR-218-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CGAAGAAGGGAAGGTAATGCTGAAAT
siRNA sets
ATTTCAGCATTACCTTCCC, TTTCAGCATTACCTTCCCT, TTCAGCATTACCTTCCCTT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCELAPOR2_348_ORF_1
0.7072
0.165586599
0.849879272
693
ORF sequence:
ATGCTGAAATCAGGCACAAATATTCTGTACTGGAGAACGACAGGCATCCTTATGGGTTCTAAGGCAGTCAAGCCCGTGCTAGTAAAGAATATCACCATCGAAGGGGTGGCATACACCTCAGAGTGCTTTCCATGTAAGCCAGGCACTTTCAGCAACAAACCAGGCTCATTTAACTGCCAGATGTGTCCCAGAAACACCTATTCTGAGAAGGGTGCTAAAGAATGTATTAGGTGTAAGGAGGACTCCCAGTTTTCAGAGGAAGGAGCCAGTGAGTGTGTGGATCGCCCACCCTGCACTACCAAAGACTATTTCCAGATCCATACCCCCTGTGACGAAGAAGGGAAGGTAATGCTGAAATCAGGCACAAATATTCTGTACTGGAGAACGACAGGCATCCTTATGGGTTCTAAGGCAGTCAAGCCCGTGCTAGTAAAGAATATCACCATCGAAGGGGTGGCATACACCTCAGAGTGCTTTCCATGTAAGCCAGGCACTTTCAGCAACAAACCAGGCTCATTTAACTGCCAGATGTGTCCCAGAAACACCTATTCTGAGAAGGGTGCTAAAGAATGTATTAGGTGTAAGGAGGACTCCCAGTTTTCAGAGGAAGGAGCCAGTGAGTGTGTGGATCGCCCACCCTGCACTACCAAAGACTATTTCCAGATCCATACCCCCTGTGACGAAGAAGGGAAG
Peptide sequence:
MLKSGTNILYWRTTGILMGSKAVKPVLVKNITIEGVAYTSECFPCKPGTFSNKPGSFNCQMCPRNTYSEKGAKECIRCKEDSQFSEEGASECVDRPPCTTKDYFQIHTPCDEEGKVMLKSGTNILYWRTTGILMGSKAVKPVLVKNITIEGVAYTSECFPCKPGTFSNKPGSFNCQMCPRNTYSEKGAKECIRCKEDSQFSEEGASECVDRPPCTTKDYFQIHTPCDEEGK
IRESfinder result
IRESfinder score
0.517393548
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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