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mmu_circElapor2_310_2
Basic information of circular RNA
PanCircBase ID:
mmu_circElapor2_310_2
mm39_Chr. Position ID:
chr5|9477689|9478533|+
Gene name:
Elapor2
Transcript:
ENSMUST00000155764.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
9,10
Exon/intron offsets:
0,715
Genomic length:
844
Exon/intron sizes in nt:
1,81,129
Flanking exon/introns:
chr5:9472390-9477689|chr5:9478533-9484620
Splice length:
310
Other database IDs:
CIRCpedia: MMU_CIRCpedia_34740, circAtlas: mmu-9330182L06Rik_0005
circBase: hsa_circ_0134912, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_59151
Human Ortholog IDs:
Mature spliced Sequence:
ACACAGATAATGTACAAGTGGATAGAGCCAAAAATCTGCAGGGAGGATCTCACAGATGCTATTAGGCTGCCCCCTTCTGGAGAGAAGAAGGATTGTCCTCCTTGCAACCCAGGATTCTATAACAATGGATCGTCTTCTTGCCATCCCTGCCCTCCGGGGACGTTTTCAGATGGAACAAAGGAATGCAAGTCATGTCCGGCCGGAACAGAGCCAGCACTTGGGTTTGAATATAAATGGTGGAATGTCCTCCCTGCCAACATGAAAACTTCCTGCTTCAATGTTGGGAATTCCAAGTGCGATGGAATGAACG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATGTCCTCCCTGCCAACATG
60.03
GAAGGGGGCAGCCTAATAGC
60.25
146
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTCCTCCCTGCCAACATGAA
59.96
GAAGGGGGCAGCCTAATAGC
60.25
144
PCR template sequence:
CCAGCACTTGGGTTTGAATATAAATGGTGGAATGTCCTCCCTGCCAACATGAAAACTTCCTGCTTCAATGTTGGGAATTCCAAGTGCGATGGAATGAACGACACAGATAATGTACAAGTGGATAGAGCCAAAAATCTGCAGGGAGGATCTCACAGATGCTATTAGGCTGCCCCCTTCTGGAGAGAAGAAGGATTGTCCTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-135a-5p(1), mmu-miR-31-5p(1), mmu-miR-149-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GATGGAATGAACGACACAGATAATGT
siRNA sets
TATCTGTGTCGTTCATTCC, ATCTGTGTCGTTCATTCCA, ATTATCTGTGTCGTTCATT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCELAPOR2_310_2_ORF_1
0.8893
0.028305803
0.300078018
366
ORF sequence:
ATGTACAAGTGGATAGAGCCAAAAATCTGCAGGGAGGATCTCACAGATGCTATTAGGCTGCCCCCTTCTGGAGAGAAGAAGGATTGTCCTCCTTGCAACCCAGGATTCTATAACAATGGATCGTCTTCTTGCCATCCCTGCCCTCCGGGGACGTTTTCAGATGGAACAAAGGAATGCAAGTCATGTCCGGCCGGAACAGAGCCAGCACTTGGGTTTGAATATAAATGGTGGAATGTCCTCCCTGCCAACATGAAAACTTCCTGCTTCAATGTTGGGAATTCCAAGTGCGATGGAATGAACGACACAGATAATGTACAAGTGGATAGAGCCAAAAATCTGCAGGGAGGATCTCACAGATGCTATTAG
Peptide sequence:
MYKWIEPKICREDLTDAIRLPPSGEKKDCPPCNPGFYNNGSSSCHPCPPGTFSDGTKECKSCPAGTEPALGFEYKWWNVLPANMKTSCFNVGNSKCDGMNDTDNVQVDRAKNLQGGSHRCY
IRESfinder result
IRESfinder score
0.36249303
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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