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mmu_circEif4enif1_637
Basic information of circular RNA
PanCircBase ID:
mmu_circEif4enif1_637
mm39_Chr. Position ID:
chr11|3179868|3186353|+
Gene name:
Eif4enif1
Transcript:
ENSMUST00000020734.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
10,11,12,13
Exon/intron offsets:
0,4593,5712,6345
Genomic length:
6485
Exon/intron sizes in nt:
23,31,84,80,140
Flanking exon/introns:
chr11:3177885-3179868|chr11:3186353-3189400
Splice length:
637
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CACATTCAGGGGTTGTACTTTCAGTGGAAGAGGTAGAAGCAGGGCTGAAGGGCTTGAAAGTGGACCAGCAAATGAAGAATTCAACTCCCTTCATGGCAGAACATTTAGAGGAGACCCTGAGTGCTGCGAGTAGCAATAGACAGCTCAAGAAAGATGGAGATATGACTGCATTCAACAAGCTAGTGAACACCATGAAGGCAAGTGGAACTTTGCCTACCCAGCCCAAAGTCAGTGAACTTCTGGGTCAACCGGTTCAGAGACCCGCTTCTTCCAATCTTCTCAGTGGCCTTATGGGGAGCTTGGAGGCTACTGCATCTTTATTGAGCCAAAGAGCGCCCTCTCCTCCAATGTCACAGGTATTTCGAACTCAAGCAGCCTCAGCAGACTACCTTCATCCAAGGATACCATCACCAATAGGTTTCCCTTCAGGACCCCAGCAACTACTTGGAGATCCATTCCAAGGCATGCGCAAGCCCATGAGTCCTGTCTCAGCCCAGATGAGCCAGCTAGAATTGCAGCAAGCTGCTTTGGAGGGGCTGGCCCTACCACATGACCTTGCAGTCCAGACAGCACCCTTCTACCAGCCTGGTTTTAGCAAACCACAAGTGGACAGAACCAGAGATGGGCTCAGAAACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCACATGACCTTGCAGTCCA
60.25
TCAGCCCTGCTTCTACCTCT
59.96
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCACATGACCTTGCAGTCC
60.25
TCAGCCCTGCTTCTACCTCT
59.96
141
PCR template sequence:
TGGCCCTACCACATGACCTTGCAGTCCAGACAGCACCCTTCTACCAGCCTGGTTTTAGCAAACCACAAGTGGACAGAACCAGAGATGGGCTCAGAAACAGCACATTCAGGGGTTGTACTTTCAGTGGAAGAGGTAGAAGCAGGGCTGAAGGGCTTGAAAGTGGACCAGCAAATGAAGAATTCAACTCCCTTCATGGCAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-196a-5p(1), mmu-miR-136-5p(1), mmu-miR-362-5p(1), mmu-miR-342-5p(1), mmu-miR-532-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGCTCAGAAACAGCACATTCAGGGGT
siRNA sets
GAATGTGCTGTTTCTGAGC, TGAATGTGCTGTTTCTGAG, AATGTGCTGTTTCTGAGCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEIF4ENIF1_637_ORF_1
1.0109
0.115782625
0.908696049
723
ORF sequence:
ATGACCTTGCAGTCCAGACAGCACCCTTCTACCAGCCTGGTTTTAGCAAACCACAAGTGGACAGAACCAGAGATGGGCTCAGAAACAGCACATTCAGGGGTTGTACTTTCAGTGGAAGAGGTAGAAGCAGGGCTGAAGGGCTTGAAAGTGGACCAGCAAATGAAGAATTCAACTCCCTTCATGGCAGAACATTTAGAGGAGACCCTGAGTGCTGCGAGTAGCAATAGACAGCTCAAGAAAGATGGAGATATGACTGCATTCAACAAGCTAGTGAACACCATGAAGGCAAGTGGAACTTTGCCTACCCAGCCCAAAGTCAGTGAACTTCTGGGTCAACCGGTTCAGAGACCCGCTTCTTCCAATCTTCTCAGTGGCCTTATGGGGAGCTTGGAGGCTACTGCATCTTTATTGAGCCAAAGAGCGCCCTCTCCTCCAATGTCACAGGTATTTCGAACTCAAGCAGCCTCAGCAGACTACCTTCATCCAAGGATACCATCACCAATAGGTTTCCCTTCAGGACCCCAGCAACTACTTGGAGATCCATTCCAAGGCATGCGCAAGCCCATGAGTCCTGTCTCAGCCCAGATGAGCCAGCTAGAATTGCAGCAAGCTGCTTTGGAGGGGCTGGCCCTACCACATGACCTTGCAGTCCAGACAGCACCCTTCTACCAGCCTGGTTTTAGCAAACCACAAGTGGACAGAACCAGAGATGGGCTCAGAAAC
Peptide sequence:
MTLQSRQHPSTSLVLANHKWTEPEMGSETAHSGVVLSVEEVEAGLKGLKVDQQMKNSTPFMAEHLEETLSAASSNRQLKKDGDMTAFNKLVNTMKASGTLPTQPKVSELLGQPVQRPASSNLLSGLMGSLEATASLLSQRAPSPPMSQVFRTQAASADYLHPRIPSPIGFPSGPQQLLGDPFQGMRKPMSPVSAQMSQLELQQAALEGLALPHDLAVQTAPFYQPGFSKPQVDRTRDGLRN
IRESfinder result
IRESfinder score
0.56995033
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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