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mmu_circEif4enif1_476
Basic information of circular RNA
PanCircBase ID:
mmu_circEif4enif1_476
mm39_Chr. Position ID:
chr11|3183987|3186353|+
Gene name:
Eif4enif1
Transcript:
ENSMUST00000110049.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
11,12,13,14
Exon/intron offsets:
0,474,1593,2226
Genomic length:
2366
Exon/intron sizes in nt:
7,21,84,80,140
Flanking exon/introns:
chr11:3180101-3183987|chr11:3186353-3189400
Splice length:
476
Other database IDs:
CIRCpedia: MMU_CIRCpedia_218602, circAtlas: mmu-Eif4enif1_0012
circBase: hsa_circ_0003630, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_36396
Human Ortholog IDs:
Mature spliced Sequence:
CGAAACGTTGAAAGTCACTTATTGGCCCCTGCTGAGATTCCAGGCCAGCCTGTTTCTAAAAACATCCTACAGGAACTTCTGGGTCAACCGGTTCAGAGACCCGCTTCTTCCAATCTTCTCAGTGGCCTTATGGGGAGCTTGGAGGCTACTGCATCTTTATTGAGCCAAAGAGCGCCCTCTCCTCCAATGTCACAGGTATTTCGAACTCAAGCAGCCTCAGCAGACTACCTTCATCCAAGGATACCATCACCAATAGGTTTCCCTTCAGGACCCCAGCAACTACTTGGAGATCCATTCCAAGGCATGCGCAAGCCCATGAGTCCTGTCTCAGCCCAGATGAGCCAGCTAGAATTGCAGCAAGCTGCTTTGGAGGGGCTGGCCCTACCACATGACCTTGCAGTCCAGACAGCACCCTTCTACCAGCCTGGTTTTAGCAAACCACAAGTGGACAGAACCAGAGATGGGCTCAGAAACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCAGACAGCACCCTTCTACC
59.75
TGAACCGGTTGACCCAGAAG
59.89
170
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCAGACAGCACCCTTCTACC
59.75
CTGAACCGGTTGACCCAGAA
59.89
171
PCR template sequence:
TGGCCCTACCACATGACCTTGCAGTCCAGACAGCACCCTTCTACCAGCCTGGTTTTAGCAAACCACAAGTGGACAGAACCAGAGATGGGCTCAGAAACAGCGAAACGTTGAAAGTCACTTATTGGCCCCTGCTGAGATTCCAGGCCAGCCTGTTTCTAAAAACATCCTACAGGAACTTCTGGGTCAACCGGTTCAGAGAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-216a-5p(1), mmu-miR-196a-5p(1), mmu-miR-214-3p(1), mmu-miR-362-5p(1), mmu-miR-342-5p(1), mmu-miR-532-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGCTCAGAAACAGCGAAACGTTGAAA
siRNA sets
TTTCAACGTTTCGCTGTTT, CAACGTTTCGCTGTTTCTG, TTCAACGTTTCGCTGTTTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEIF4ENIF1_476_ORF_1
0.6561
0.013484152
0.423158649
510
ORF sequence:
ATGGGGAGCTTGGAGGCTACTGCATCTTTATTGAGCCAAAGAGCGCCCTCTCCTCCAATGTCACAGGTATTTCGAACTCAAGCAGCCTCAGCAGACTACCTTCATCCAAGGATACCATCACCAATAGGTTTCCCTTCAGGACCCCAGCAACTACTTGGAGATCCATTCCAAGGCATGCGCAAGCCCATGAGTCCTGTCTCAGCCCAGATGAGCCAGCTAGAATTGCAGCAAGCTGCTTTGGAGGGGCTGGCCCTACCACATGACCTTGCAGTCCAGACAGCACCCTTCTACCAGCCTGGTTTTAGCAAACCACAAGTGGACAGAACCAGAGATGGGCTCAGAAACAGCGAAACGTTGAAAGTCACTTATTGGCCCCTGCTGAGATTCCAGGCCAGCCTGTTTCTAAAAACATCCTACAGGAACTTCTGGGTCAACCGGTTCAGAGACCCGCTTCTTCCAATCTTCTCAGTGGCCTTATGGGGAGCTTGGAGGCTACTGCATCTTTATTGA
Peptide sequence:
MGSLEATASLLSQRAPSPPMSQVFRTQAASADYLHPRIPSPIGFPSGPQQLLGDPFQGMRKPMSPVSAQMSQLELQQAALEGLALPHDLAVQTAPFYQPGFSKPQVDRTRDGLRNSETLKVTYWPLLRFQASLFLKTSYRNFWVNRFRDPLLPIFSVALWGAWRLLHLY
IRESfinder result
IRESfinder score
0.632890015
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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