top of page
mmu_circEif4enif1_1342
Basic information of circular RNA
PanCircBase ID:
mmu_circEif4enif1_1342
mm39_Chr. Position ID:
chr11|3165591|3180101|+
Gene name:
Eif4enif1
Transcript:
ENSMUST00000020734.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
4,5,6,7,8,9,10
Exon/intron offsets:
0,4652,5501,9040,11803,12114,14277
Genomic length:
14510
Exon/intron sizes in nt:
12,82,87,20,21,76,13,60,00,000
Flanking exon/introns:
chr11:3163333-3165591|chr11:3180101-3184461
Splice length:
1342
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TGATGGTGTCTGGGACCCTGAGAAGTGGCATGCCTCTCTCTACCCAGCCTCGGGGCGGAGCTCACCAGTGGAAAGTCTGAAGAAAGAGTCAGAATCCGATCGACCTTCTCTGGTGCGCAGAATAGCAGATCCACGAGAGCGAGTGAAAGAAGATGACTTAGATGTTGTTCTCAGCCCACAGAGACGAAGCTTTGGAGGTGGCTGCCATGTGACAGCTGCTGTTAGCTCCCGACGATCTGGCAGTCCATTGGAGAAAGACAGTGATGGACTTCGCCTGCTTGGTGGACGAAGAATTGGCAGTGGAAGGATAATTTCTGCCCGGGCCTTTGAAAAGGATCACCGTCTCAGTGATAAGGACCTGCGAGACTTGAGAGACCGAGACAGAGAGAGGGACTACAAGGACAAACGTTTCAGGAGGGAGTTTGGAGATAGTAAACGTGTCTTTGGTGAGCGTAGAAGAAACGATTCATACACTGAGGAAGAACCAGAATGGTTTTCAGCTGGACCCACCAGTCAGTCTGAAACCATAGAGCTGACTGGCTTTGATGATAAGATTCTAGAGGAGGATCACAAAGGCAGAAAAAGAACAAGGCGACGGACTGCCTCTGTGAAGGAAGGCATAGTGGAGTGCAATGGAGGGGTGGCTGAAGAGGACGAGGTGGAAGTCATTCTGGCCCAGGAGCCCTCTGCTGATCAGGAAGTTCCAAGGGATGTCATCCTACCTGAGCAGTCCCCAGGAGAATTTGACTTTAATGAATTCTTTAACCTTGATAAGGTGCCATGCTTGGCTTCGATGATAGAAGATGTTCTGGGGGAAGGATCGGTCTCTGCCAGCCGCTTCAGTAGGTGGTTCTCTAACCCAAGCCGGTCAGGGAGCAGATCCAGCAGCCTTGGATCCACACCCCATGAAGAACTTGAGAGACTGGCAGGTTTGGAGCAAGCCGTCCTCTCTCCTGGACAGAACTCGGGGAATTATTTTGCTCCTATACCATCGGAAGACCATGCTGAAAATAAAGTGGACATTTTAGAAATGCTACAGAAAGCCAAAGTAGATTTAAAACCTCTTCTTTCTAGCCTTTCTGCAAATAAAGAAAAACTTAAGGAGAGCTCACATTCAGGGGTTGTACTTTCAGTGGAAGAGGTAGAAGCAGGGCTGAAGGGCTTGAAAGTGGACCAGCAAATGAAGAATTCAACTCCCTTCATGGCAGAACATTTAGAGGAGACCCTGAGTGCTGCGAGTAGCAATAGACAGCTCAAGAAAGATGGAGATATGACTGCATTCAACAAGCTAGTGAACACCATGAAGGCAAGTGGAACTTTGCCTACCCAGCCCAAAGTCAGT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGACTGCATTCAACAAGCTAGTG
59.5
ACTTTCCACTGGTGAGCTCC
59.6
147
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGACAGCTCAAGAAAGATGGAGA
59.16
GGTAGAGAGAGGCATGCCAC
59.89
140
PCR template sequence:
CAATAGACAGCTCAAGAAAGATGGAGATATGACTGCATTCAACAAGCTAGTGAACACCATGAAGGCAAGTGGAACTTTGCCTACCCAGCCCAAAGTCAGTTGATGGTGTCTGGGACCCTGAGAAGTGGCATGCCTCTCTCTACCCAGCCTCGGGGCGGAGCTCACCAGTGGAAAGTCTGAAGAAAGAGTCAGAATCCGAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-32-5p(1), mmu-miR-92a-3p(1), mmu-miR-25-3p(1), mmu-miR-185-5p(1), mmu-miR-136-5p(1), mmu-miR-433-5p(1), mmu-miR-431-5p(1), mmu-miR-98-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-539-5p(1), mmu-miR-670-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GCCCAAAGTCAGTTGATGGTGTCTGG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEIF4ENIF1_1342_ORF_1
0.8482
0.099782982
0.659908502
552
ORF sequence:
ATGATAGAAGATGTTCTGGGGGAAGGATCGGTCTCTGCCAGCCGCTTCAGTAGGTGGTTCTCTAACCCAAGCCGGTCAGGGAGCAGATCCAGCAGCCTTGGATCCACACCCCATGAAGAACTTGAGAGACTGGCAGGTTTGGAGCAAGCCGTCCTCTCTCCTGGACAGAACTCGGGGAATTATTTTGCTCCTATACCATCGGAAGACCATGCTGAAAATAAAGTGGACATTTTAGAAATGCTACAGAAAGCCAAAGTAGATTTAAAACCTCTTCTTTCTAGCCTTTCTGCAAATAAAGAAAAACTTAAGGAGAGCTCACATTCAGGGGTTGTACTTTCAGTGGAAGAGGTAGAAGCAGGGCTGAAGGGCTTGAAAGTGGACCAGCAAATGAAGAATTCAACTCCCTTCATGGCAGAACATTTAGAGGAGACCCTGAGTGCTGCGAGTAGCAATAGACAGCTCAAGAAAGATGGAGATATGACTGCATTCAACAAGCTAGTGAACACCATGAAGGCAAGTGGAACTTTGCCTACCCAGCCCAAAGTCAGTTGA
Peptide sequence:
MIEDVLGEGSVSASRFSRWFSNPSRSGSRSSSLGSTPHEELERLAGLEQAVLSPGQNSGNYFAPIPSEDHAENKVDILEMLQKAKVDLKPLLSSLSANKEKLKESSHSGVVLSVEEVEAGLKGLKVDQQMKNSTPFMAEHLEETLSAASSNRQLKKDGDMTAFNKLVNTMKASGTLPTQPKVS
IRESfinder result
IRESfinder score
0.544562557
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page