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mmu_circEif4b_639
Basic information of circular RNA
PanCircBase ID:
mmu_circEif4b_639
mm39_Chr. Position ID:
chr15|101997050|101999929|+
Gene name:
Eif4b
Transcript:
ENSMUST00000169681.3
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
7,8,9,10
Exon/intron offsets:
0,241,1250,2781
Genomic length:
2879
Exon/intron sizes in nt:
138,174,229,98
Flanking exon/introns:
chr15:101995074-101997050|chr15:101999929-102001420
Splice length:
639
Other database IDs:
CIRCpedia: MMU_CIRCpedia_646
circBase: hsa_circ_0026523, CIRCpedia: HSA_CIRCpedia_176706
Human Ortholog IDs:
Mature spliced Sequence:
AGTATCGAGATCGTTACGATTCAGACCGGTATCGGGATGGGTATAGGGACGGATATCGGGACGGCCCACGCAGAGACATGGACCGCTATGGGGGCCGGGATCGCTATGATGACCGAGGCAGCAGAGACTATGACCGAGGCTATGACTCCAGGATAGGCAGTGGCAGAAGGGCATTTGGAAGTGGGTACCGGAGAGATGATGACTACAGAGGAGGTGGGGACCGCTATGAAGACCGCTATGACAGACGGGATGATCGGTCGTGGAGCTCCAGGGATGACTACTCTCGGGATGATTATAGGCGTGATGACAGAGGTCCCCCCCAGAGACCCAGACTGAACCTCAAGCCTCGAAGCGCTCCTAAGGAGGATGACGCCTCCGCCAGCACCTCCCAGTCCAGCCGGGCAGCCTCCATCTTTGGAGGGGCGAAGCCTGTTGACACAGCTGCTAGGGAAAGAGAAGTAGAGGAGCGGCTACAGAAGGAGCAGGAGAAGCTGCAGCGTCAGCTGGATGAGCCAAAACTAGACCGCCGGCCCCGGGAGAGACACCCAAGCTGGCGAAGTGAAGAAACTCAGGAAAGAGAACGGTCAAGGACAGGAAGTGAGTCATCGCAGACTGGGGCCTCAGCCACATCTGGCAGAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACGGTCAAGGACAGGAAGTG
59.61
CCCATAGCGGTCCATGTCTC
59.97
151
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGGTCAAGGACAGGAAGTGA
59.32
CCCATAGCGGTCCATGTCTC
59.97
150
PCR template sequence:
AGACACCCAAGCTGGCGAAGTGAAGAAACTCAGGAAAGAGAACGGTCAAGGACAGGAAGTGAGTCATCGCAGACTGGGGCCTCAGCCACATCTGGCAGAAAGTATCGAGATCGTTACGATTCAGACCGGTATCGGGATGGGTATAGGGACGGATATCGGGACGGCCCACGCAGAGACATGGACCGCTATGGGGGCCGGGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-207(1), mmu-miR-3087-3p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
ACATCTGGCAGAAAGTATCGAGATCG
siRNA sets
GATCTCGATACTTTCTGCC, ATCTCGATACTTTCTGCCA, CGATCTCGATACTTTCTGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEIF4B_639_ORF_1
0.9947
0.463104548
0.999199773
1200
ORF sequence:
ATGGACCGCTATGGGGGCCGGGATCGCTATGATGACCGAGGCAGCAGAGACTATGACCGAGGCTATGACTCCAGGATAGGCAGTGGCAGAAGGGCATTTGGAAGTGGGTACCGGAGAGATGATGACTACAGAGGAGGTGGGGACCGCTATGAAGACCGCTATGACAGACGGGATGATCGGTCGTGGAGCTCCAGGGATGACTACTCTCGGGATGATTATAGGCGTGATGACAGAGGTCCCCCCCAGAGACCCAGACTGAACCTCAAGCCTCGAAGCGCTCCTAAGGAGGATGACGCCTCCGCCAGCACCTCCCAGTCCAGCCGGGCAGCCTCCATCTTTGGAGGGGCGAAGCCTGTTGACACAGCTGCTAGGGAAAGAGAAGTAGAGGAGCGGCTACAGAAGGAGCAGGAGAAGCTGCAGCGTCAGCTGGATGAGCCAAAACTAGACCGCCGGCCCCGGGAGAGACACCCAAGCTGGCGAAGTGAAGAAACTCAGGAAAGAGAACGGTCAAGGACAGGAAGTGAGTCATCGCAGACTGGGGCCTCAGCCACATCTGGCAGAAAGTATCGAGATCGTTACGATTCAGACCGGTATCGGGATGGGTATAGGGACGGATATCGGGACGGCCCACGCAGAGACATGGACCGCTATGGGGGCCGGGATCGCTATGATGACCGAGGCAGCAGAGACTATGACCGAGGCTATGACTCCAGGATAGGCAGTGGCAGAAGGGCATTTGGAAGTGGGTACCGGAGAGATGATGACTACAGAGGAGGTGGGGACCGCTATGAAGACCGCTATGACAGACGGGATGATCGGTCGTGGAGCTCCAGGGATGACTACTCTCGGGATGATTATAGGCGTGATGACAGAGGTCCCCCCCAGAGACCCAGACTGAACCTCAAGCCTCGAAGCGCTCCTAAGGAGGATGACGCCTCCGCCAGCACCTCCCAGTCCAGCCGGGCAGCCTCCATCTTTGGAGGGGCGAAGCCTGTTGACACAGCTGCTAGGGAAAGAGAAGTAGAGGAGCGGCTACAGAAGGAGCAGGAGAAGCTGCAGCGTCAGCTGGATGAGCCAAAACTAGACCGCCGGCCCCGGGAGAGACACCCAAGCTGGCGAAGTGAAGAAACTCAGGAAAGAGAACGGTCAAGGACAGGAAGTGAGTCATCGCAGACTGGGGCCTCAGCCACATCTGGCAGA
Peptide sequence:
MDRYGGRDRYDDRGSRDYDRGYDSRIGSGRRAFGSGYRRDDDYRGGGDRYEDRYDRRDDRSWSSRDDYSRDDYRRDDRGPPQRPRLNLKPRSAPKEDDASASTSQSSRAASIFGGAKPVDTAAREREVEERLQKEQEKLQRQLDEPKLDRRPRERHPSWRSEETQERERSRTGSESSQTGASATSGRKYRDRYDSDRYRDGYRDGYRDGPRRDMDRYGGRDRYDDRGSRDYDRGYDSRIGSGRRAFGSGYRRDDDYRGGGDRYEDRYDRRDDRSWSSRDDYSRDDYRRDDRGPPQRPRLNLKPRSAPKEDDASASTSQSSRAASIFGGAKPVDTAAREREVEERLQKEQEKLQRQLDEPKLDRRPRERHPSWRSEETQERERSRTGSESSQTGASATSGR
IRESfinder result
IRESfinder score
0.084187476
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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