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mmu_circEif4b_190
Basic information of circular RNA
PanCircBase ID:
mmu_circEif4b_190
mm39_Chr. Position ID:
chr15|101994437|101995074|+
Gene name:
Eif4b
Transcript:
ENSMUST00000169681.3
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
5,6
Exon/intron offsets:
0,502
Genomic length:
637
Exon/intron sizes in nt:
55,135
Flanking exon/introns:
chr15:101993230-101994437|chr15:101995074-101997050
Splice length:
190
Other database IDs:
CIRCpedia: MMU_CIRCpedia_644, circAtlas: mmu-Eif4b_0004
circBase: hsa_circ_0026515, CIRCpedia: HSA_CIRCpedia_8866
Human Ortholog IDs:
Mature spliced Sequence:
TCTCTAGGTAACAGGAGAATTCGTGTGGATGTTGCTGATCAAGCACAGGATAAAGACAGGGATGACCGTTCTTTTGGTCGAGATAGAAATCGGGATTCTGACAAAACAGACACAGACTGGAGGGCCCGTCCCACCACAGACAGTTTTGATGACTACCCACCTAGAAGAGGCGATGATAGCTTTGGAGACA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGTCCCACCACAGACAGTTT
60.18
ACCAAAAGAACGGTCATCCCT
59.58
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGTCCCACCACAGACAGTTT
60.18
AGAACGGTCATCCCTGTCTT
58.35
136
PCR template sequence:
TTCTGACAAAACAGACACAGACTGGAGGGCCCGTCCCACCACAGACAGTTTTGATGACTACCCACCTAGAAGAGGCGATGATAGCTTTGGAGACATCTCTAGGTAACAGGAGAATTCGTGTGGATGTTGCTGATCAAGCACAGGATAAAGACAGGGATGACCGTTCTTTTGGTCGAGATAGAAATCGGGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-218-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AGCTTTGGAGACATCTCTAGGTAACA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEIF4B_190_ORF_1
0.9639
0.19258252
0.465260873
351
ORF sequence:
ATGTTGCTGATCAAGCACAGGATAAAGACAGGGATGACCGTTCTTTTGGTCGAGATAGAAATCGGGATTCTGACAAAACAGACACAGACTGGAGGGCCCGTCCCACCACAGACAGTTTTGATGACTACCCACCTAGAAGAGGCGATGATAGCTTTGGAGACATCTCTAGGTAACAGGAGAATTCGTGTGGATGTTGCTGATCAAGCACAGGATAAAGACAGGGATGACCGTTCTTTTGGTCGAGATAGAAATCGGGATTCTGACAAAACAGACACAGACTGGAGGGCCCGTCCCACCACAGACAGTTTTGATGACTACCCACCTAGAAGAGGCGATGATAGCTTTGGAGAC
Peptide sequence:
MLLIKHRIKTGMTVLLVEIEIGILTKQTQTGGPVPPQTVLMTTHLEEAMIALETSLGNRRIRVDVADQAQDKDRDDRSFGRDRNRDSDKTDTDWRARPTTDSFDDYPPRRGDDSFGD
IRESfinder result
IRESfinder score
0.434434561
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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