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mmu_circEif3b_304
Basic information of circular RNA
PanCircBase ID:
mmu_circEif3b_304
mm39_Chr. Position ID:
chr5|140427491|140428191|+
Gene name:
Eif3b
Transcript:
ENSMUST00000100507.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
16,17,18
Exon/intron offsets:
0,387,583
Genomic length:
700
Exon/intron sizes in nt:
7,81,09,117
Flanking exon/introns:
chr5:140426866-140427491|chr5:140428191-140428628
Splice length:
304
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
CAAATTAAAAAGGATCTGAAGAAGTACTCCAAGATCTTTGAGCAGAAGGATCGCTTGAGCCAGTCCAAAGCTTCAAAGGAACTGGTGGAAAGAAGGCGGACCATGATGGAGGACTTCCGGCAATACCGGAAAATGGCCCAGGAACTCTACATGAAGCAGAAGAACGAGCGTCTAGAGCTACGAGGAGGGGTGGACACTGACGAGCTGGACAGCAACGTGGATGACTGGGAGGAAGAGACCATTGAGTTTTTTGTCACCGAGGAAGTCATTCCTCTGGGAAGTCAGGAGTGATCTCAGCACTGTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGTGGATGACTGGGAGGAAG
59.82
TTTGGACTGGCTCAAGCGAT
59.96
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGTGGATGACTGGGAGGAAG
59.82
ACTGGCTCAAGCGATCCTTC
60.11
153
PCR template sequence:
CTGGACAGCAACGTGGATGACTGGGAGGAAGAGACCATTGAGTTTTTTGTCACCGAGGAAGTCATTCCTCTGGGAAGTCAGGAGTGATCTCAGCACTGTGCAAATTAAAAAGGATCTGAAGAAGTACTCCAAGATCTTTGAGCAGAAGGATCGCTTGAGCCAGTCCAAAGCTTCAAAGGAACTGGTGGAAAGAAGGCGGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-128-3p(1), mmu-miR-149-5p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
TCTCAGCACTGTGCAAATTAAAAAGG
siRNA sets
TTTTAATTTGCACAGTGCT, TTTAATTTGCACAGTGCTG, TTAATTTGCACAGTGCTGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEIF3B_304_ORF_1
1.1421
0.396176208
0.767184904
375
ORF sequence:
ATGACTGGGAGGAAGAGACCATTGAGTTTTTTGTCACCGAGGAAGTCATTCCTCTGGGAAGTCAGGAGTGATCTCAGCACTGTGCAAATTAAAAAGGATCTGAAGAAGTACTCCAAGATCTTTGAGCAGAAGGATCGCTTGAGCCAGTCCAAAGCTTCAAAGGAACTGGTGGAAAGAAGGCGGACCATGATGGAGGACTTCCGGCAATACCGGAAAATGGCCCAGGAACTCTACATGAAGCAGAAGAACGAGCGTCTAGAGCTACGAGGAGGGGTGGACACTGACGAGCTGGACAGCAACGTGGATGACTGGGAGGAAGAGACCATTGAGTTTTTTGTCACCGAGGAAGTCATTCCTCTGGGAAGTCAGGAGTGA
Peptide sequence:
MTGRKRPLSFLSPRKSFLWEVRSDLSTVQIKKDLKKYSKIFEQKDRLSQSKASKELVERRRTMMEDFRQYRKMAQELYMKQKNERLELRGGVDTDELDSNVDDWEEETIEFFVTEEVIPLGSQE
IRESfinder result
IRESfinder score
0.285711879
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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