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mmu_circEif3a_1304
Basic information of circular RNA
PanCircBase ID:
mmu_circEif3a_1304
mm39_Chr. Position ID:
chr19|60756012|60762130|-
Gene name:
Eif3a
Transcript:
ENSMUST00000025955.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
18,17,16,15,14,13,12,11
Exon/intron offsets:
0,1133,2291,3440,4069,4518,5117,5932
Genomic length:
6118
Exon/intron sizes in nt:
89,18,51,54,12,31,14,10,00,00,000
Flanking exon/introns:
chr19:60755433-60756012|chr19:60762130-60762338
Splice length:
1304
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTACGTATTGACCATACTTCCCGGACTCTGAGTTTTGGATCAGATTTGAATTATGCAACTCGAGAAGATGCCCCAGTTGGCCCTCATCTGCAGAGCATGCCTTCAGAGCAGATAAGAAACCAGCTCACGGCCATGTCCTCAGTGCTTGCCAAAGCACTTGAAGTCATCAGACCTGCTCACATTCTGCAAGAGAAAGAAGAACAGCATCAATTGGCAGTTAATGCATATCTTAAAAATTCAAGAAAAGAGCACCAGAGGATCCTGGCTCGGAGACAGACAATTGAGGAAAGAAAAGAGCGTCTTGAGAGTCTGAATATTCAACGTGAGAAGGAAGAACTCGAGCAGAGGGAAGCTGAACTCCAGAAAGTACGAAAGGCTGAAGAAGAAAGGCTGCGCCAAGAGGCAAAGGAGCGAGAGAAGGAACGAATCCTTCAAGAACACGAGCAAATCAAGAAGAAAACTGTTCGGGAGCGGTTAGAGCAGATCAAGAAGACAGAGCTGGGCGCCAAAGCATTTAAAGATATTGACATTGAAGACCTTGAAGAACTGGATCCAGATTTTATTATGGCCAAACAGGTTGAACAACTGGAGAAAGAAAAGAAGGAACTTCAGGAACGCCTGAAGAATCAAGAAAAGAAGATTGACTATTTTGAGAGAGCTAAGCGTTTGGAAGAAATTCCTTTAATAAAGAGTGCTTATGAGGAACAAAGGGTTAAAGACATGGACCTGTGGGAACAGCAAGAAGAAGAAAGAATCACTACAATGCAGCTAGAACGAGAAAAAGCTCTGGAGCATAAGAATAGGATGTCACGAATGTTGGAAGACAGAGATCTATTTGTGATGCGCCTCAAAGCTGCCCGGCAGTCTGTCTACGAGGAAAAACTGAAACAGTTTGAAGAGCGCTTAGCAGAAGAAAGGCATAGTCGCCTAGAAGATCGGAAAAGGCAGCGGAAAGAAGAACGCAAAATAACTTATTACAGAGAAAAGGAAGAAGAAGAGCAGAGGAGGGCAGAGGAGCAGATGCTCAAAGAGCGAGAAGAAAGAGAACGTGCTGAGAGAGCAAAACGGGAGGAAGAACTTAGAGAGTATCAGGAGCGAGTCAAGAAACTAGAAGAAGTAGAAAGGAAAAAGCGGCAAAGAGAGCTGGAAATTGAAGAAAGGGAAAGGCGCAGAGAGGAAGAAAGGAGACTTGGTGATGATCCACTTTCTAGGAAGGACTCTCGGTGGGGAGATAGAGATTCAGAAGGCACCTGGAGGAAAGGACCAGAAGCTGACTCTGAGTGGAGAAGAGGCCCACCAGAAAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TAGGAAGGACTCTCGGTGGG
60.03
TGGGGCATCTTCTCGAGTTG
59.75
171
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TAGGAAGGACTCTCGGTGGG
60.03
CAACTGGGGCATCTTCTCGA
59.75
175
PCR template sequence:
TTTCTAGGAAGGACTCTCGGTGGGGAGATAGAGATTCAGAAGGCACCTGGAGGAAAGGACCAGAAGCTGACTCTGAGTGGAGAAGAGGCCCACCAGAAAAGTACGTATTGACCATACTTCCCGGACTCTGAGTTTTGGATCAGATTTGAATTATGCAACTCGAGAAGATGCCCCAGTTGGCCCTCATCTGCAGAGCATGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-33-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-22-3p(1), mmu-miR-680(1), mmu-miR-384-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-697(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
GCCCACCAGAAAAGTACGTATTGACC
siRNA sets
CAATACGTACTTTTCTGGT, GTCAATACGTACTTTTCTG, ATACGTACTTTTCTGGTGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEIF3A_1304_ORF_1
1.2299
0.328679581
0.999302972
1239
ORF sequence:
ATGCCTTCAGAGCAGATAAGAAACCAGCTCACGGCCATGTCCTCAGTGCTTGCCAAAGCACTTGAAGTCATCAGACCTGCTCACATTCTGCAAGAGAAAGAAGAACAGCATCAATTGGCAGTTAATGCATATCTTAAAAATTCAAGAAAAGAGCACCAGAGGATCCTGGCTCGGAGACAGACAATTGAGGAAAGAAAAGAGCGTCTTGAGAGTCTGAATATTCAACGTGAGAAGGAAGAACTCGAGCAGAGGGAAGCTGAACTCCAGAAAGTACGAAAGGCTGAAGAAGAAAGGCTGCGCCAAGAGGCAAAGGAGCGAGAGAAGGAACGAATCCTTCAAGAACACGAGCAAATCAAGAAGAAAACTGTTCGGGAGCGGTTAGAGCAGATCAAGAAGACAGAGCTGGGCGCCAAAGCATTTAAAGATATTGACATTGAAGACCTTGAAGAACTGGATCCAGATTTTATTATGGCCAAACAGGTTGAACAACTGGAGAAAGAAAAGAAGGAACTTCAGGAACGCCTGAAGAATCAAGAAAAGAAGATTGACTATTTTGAGAGAGCTAAGCGTTTGGAAGAAATTCCTTTAATAAAGAGTGCTTATGAGGAACAAAGGGTTAAAGACATGGACCTGTGGGAACAGCAAGAAGAAGAAAGAATCACTACAATGCAGCTAGAACGAGAAAAAGCTCTGGAGCATAAGAATAGGATGTCACGAATGTTGGAAGACAGAGATCTATTTGTGATGCGCCTCAAAGCTGCCCGGCAGTCTGTCTACGAGGAAAAACTGAAACAGTTTGAAGAGCGCTTAGCAGAAGAAAGGCATAGTCGCCTAGAAGATCGGAAAAGGCAGCGGAAAGAAGAACGCAAAATAACTTATTACAGAGAAAAGGAAGAAGAAGAGCAGAGGAGGGCAGAGGAGCAGATGCTCAAAGAGCGAGAAGAAAGAGAACGTGCTGAGAGAGCAAAACGGGAGGAAGAACTTAGAGAGTATCAGGAGCGAGTCAAGAAACTAGAAGAAGTAGAAAGGAAAAAGCGGCAAAGAGAGCTGGAAATTGAAGAAAGGGAAAGGCGCAGAGAGGAAGAAAGGAGACTTGGTGATGATCCACTTTCTAGGAAGGACTCTCGGTGGGGAGATAGAGATTCAGAAGGCACCTGGAGGAAAGGACCAGAAGCTGACTCTGAGTGGAGAAGAGGCCCACCAGAAAAGTACGTATTGACCATACTTCCCGGACTCTGA
Peptide sequence:
MPSEQIRNQLTAMSSVLAKALEVIRPAHILQEKEEQHQLAVNAYLKNSRKEHQRILARRQTIEERKERLESLNIQREKEELEQREAELQKVRKAEEERLRQEAKEREKERILQEHEQIKKKTVRERLEQIKKTELGAKAFKDIDIEDLEELDPDFIMAKQVEQLEKEKKELQERLKNQEKKIDYFERAKRLEEIPLIKSAYEEQRVKDMDLWEQQEEERITTMQLEREKALEHKNRMSRMLEDRDLFVMRLKAARQSVYEEKLKQFEERLAEERHSRLEDRKRQRKEERKITYYREKEEEEQRRAEEQMLKEREERERAERAKREEELREYQERVKKLEEVERKKRQRELEIEERERRREEERRLGDDPLSRKDSRWGDRDSEGTWRKGPEADSEWRRGPPEKYVLTILPGL
IRESfinder result
IRESfinder score
0.363785633
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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