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mmu_circEif2ak1_1107
Basic information of circular RNA
PanCircBase ID:
mmu_circEif2ak1_1107
mm39_Chr. Position ID:
chr5|143808680|143823779|+
Gene name:
Eif2ak1
Transcript:
ENSMUST00000100487.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
2,3,4,5,6,7,8,9,10
Exon/intron offsets:
0,1888,3797,5713,7572,10290,12110,12793,14987
Genomic length:
15099
Exon/intron sizes in nt:
1,59,13,43,81,00,81,10,00,00,00,000
Flanking exon/introns:
chr5:143803763-143808680|chr5:143823779-143826213
Splice length:
1107
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AGTCCGATGTCCCCGCAGAGCTCCAAGTGTTAAAAGAGCCCCTACAGCAGCCCACGTTCCCTTTCCTGGTGGCAAACCAGCTGCTGCTCGTCTCCTTACTGGAACACTTGAGCCATGTGCACGAGCCAAACCCGCTCCACTCCAAACAGGTGTTTAAATTACTGTGCCAGACTTTTATCAAGATGGGGCTGCTCTCTTCCTTTACCTGCAGTGATGAGTTTAGCTCTCTGAGACTCCACCACAACAGAGCCATCACTCATTTAATGAGGTCTGCCAAAGAGAGAGTCCGTCAGGATCCTTGTCAAGATAATTCTTACATGCAGAAAATCAGATCCAGAGAGATAGCCTTCGAAGCACAAACGTCACGCTACTTAAATGAATTTGAAGAGCTTGCCATCTTAGGGAAAGGAGGATATGGAAGAGTTTACAAGGTCCGGAACAAATTAGATGGTCAGCATTATGCAATTAAGAAAATCCTGATTAAGAGCGCAACTAAAACAGATTGTATGAAGGTGCTACGGGAAGTGAAGGTTCTGGCAGGTCTCCAGCATCCCAATATTGTTGGCTACCACACTGCGTGGATAGAACATGTTCATGTGGTTCAGCCACAAGACAGAGTTCCGATTCAACTGCCCTCTCTCGAAGTGCTGTCGGAGCAGGAAGGGGACAGAGACCAAGGTGGTGTTAAAGATAATGAAAGCAGTTCGTCCATTGTCTTTGCTGAACTCACCCCAGAAAAAGAAAAACCTTTTGGGGAGTCTGAGGTTAAAAATGAGAATAACAACCTGGTGAGCTACACGGCCAACTTAGTGGTCAGGAACAGCAGTGAAAGTGAATCGTCCATTGAGCTCCAAGAAGACGGCTTGACTGATTTGTCCGTCAGACCAGTTGTCAGGCATCAGCTGCCGCTGGGGCATAGCTCGGAATTGGAAGGGAATTTTACATCCACGGATGAGTCTTCTGAAGGCAACTTGAACCTGCTGGGGCAGACGGAGGTTCGGTACCACCTGATGTTGCACATCCAGATGCAGCTGTGTGAGCTCTCCCTGTGGGACTGGATAACTGAGAGGAACAAGCGGAGCCGGGAGTATGTGGACGAAGCTGCTT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCAGATGCAGCTGTGTGAG
60.04
TTTGCCACCAGGAAAGGGAA
59.74
163
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTGTGTGAGCTCTCCCTGTG
60.04
TTTGCCACCAGGAAAGGGAA
59.74
152
PCR template sequence:
CTGATGTTGCACATCCAGATGCAGCTGTGTGAGCTCTCCCTGTGGGACTGGATAACTGAGAGGAACAAGCGGAGCCGGGAGTATGTGGACGAAGCTGCTTAGTCCGATGTCCCCGCAGAGCTCCAAGTGTTAAAAGAGCCCCTACAGCAGCCCACGTTCCCTTTCCTGGTGGCAAACCAGCTGCTGCTCGTCTCCTTACT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-155-5p(1), mmu-miR-15a-5p(1), mmu-miR-15b-5p(1), mmu-miR-149-5p(1), mmu-miR-342-3p(1), mmu-miR-377-3p(1), mmu-miR-188-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GACGAAGCTGCTTAGTCCGATGTCCC
siRNA sets
GACATCGGACTAAGCAGCT, GGACATCGGACTAAGCAGC, CATCGGACTAAGCAGCTTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEIF2AK1_1107_ORF_1
1.0683
0.217560606
0.986222623
927
ORF sequence:
ATGGGGCTGCTCTCTTCCTTTACCTGCAGTGATGAGTTTAGCTCTCTGAGACTCCACCACAACAGAGCCATCACTCATTTAATGAGGTCTGCCAAAGAGAGAGTCCGTCAGGATCCTTGTCAAGATAATTCTTACATGCAGAAAATCAGATCCAGAGAGATAGCCTTCGAAGCACAAACGTCACGCTACTTAAATGAATTTGAAGAGCTTGCCATCTTAGGGAAAGGAGGATATGGAAGAGTTTACAAGGTCCGGAACAAATTAGATGGTCAGCATTATGCAATTAAGAAAATCCTGATTAAGAGCGCAACTAAAACAGATTGTATGAAGGTGCTACGGGAAGTGAAGGTTCTGGCAGGTCTCCAGCATCCCAATATTGTTGGCTACCACACTGCGTGGATAGAACATGTTCATGTGGTTCAGCCACAAGACAGAGTTCCGATTCAACTGCCCTCTCTCGAAGTGCTGTCGGAGCAGGAAGGGGACAGAGACCAAGGTGGTGTTAAAGATAATGAAAGCAGTTCGTCCATTGTCTTTGCTGAACTCACCCCAGAAAAAGAAAAACCTTTTGGGGAGTCTGAGGTTAAAAATGAGAATAACAACCTGGTGAGCTACACGGCCAACTTAGTGGTCAGGAACAGCAGTGAAAGTGAATCGTCCATTGAGCTCCAAGAAGACGGCTTGACTGATTTGTCCGTCAGACCAGTTGTCAGGCATCAGCTGCCGCTGGGGCATAGCTCGGAATTGGAAGGGAATTTTACATCCACGGATGAGTCTTCTGAAGGCAACTTGAACCTGCTGGGGCAGACGGAGGTTCGGTACCACCTGATGTTGCACATCCAGATGCAGCTGTGTGAGCTCTCCCTGTGGGACTGGATAACTGAGAGGAACAAGCGGAGCCGGGAGTATGTGGACGAAGCTGCTTAG
Peptide sequence:
MGLLSSFTCSDEFSSLRLHHNRAITHLMRSAKERVRQDPCQDNSYMQKIRSREIAFEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQHYAIKKILIKSATKTDCMKVLREVKVLAGLQHPNIVGYHTAWIEHVHVVQPQDRVPIQLPSLEVLSEQEGDRDQGGVKDNESSSSIVFAELTPEKEKPFGESEVKNENNNLVSYTANLVVRNSSESESSIELQEDGLTDLSVRPVVRHQLPLGHSSELEGNFTSTDESSEGNLNLLGQTEVRYHLMLHIQMQLCELSLWDWITERNKRSREYVDEAA
IRESfinder result
IRESfinder score
0.56977566
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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