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mmu_circEhbp1_1860
Basic information of circular RNA
PanCircBase ID:
mmu_circEhbp1_1860
mm39_Chr. Position ID:
chr11|22018358|22096700|-
Gene name:
Ehbp1
Transcript:
ENSMUST00000109563.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
16,15,14,13,12,11,10
Exon/intron offsets:
0,21166,26959,32063,32693,69479,78104
Genomic length:
78342
Exon/intron sizes in nt:
1,48,14,48,82,49,17,92,00,000
Flanking exon/introns:
chr11:22012887-22018358|chr11:22096700-22101066
Splice length:
1860
Other database IDs:
circAtlas: mmu-Ehbp1_0026
Human Ortholog IDs:
Mature spliced Sequence:
AACCAATCACTGAAACAACTTCACCTAAAAAACCAGAAGAATCTTTTTATAATAACAGCTGTAATCCCTTTAAAGGTGTACAGACTCCACAGTATTTGAACCCATTTGATGAACCAGAAACTTTTGTAATGATAAAGGATTCTCCACCCCAGTCTACACGAAGAAAAAACCTAAGACCTGTAGACATGAGCAAGTACCTCTATGCTGATAGTTCAAAGAGTGAAGAGGAACTGGATGAATCAAATCCTTTTTATGAGCCTAAGCCAACTTCTCCAAATAATTTGGTCAATACAGTTCAAGAAGGGGAAACTGAACGGAGAGTGAAAAGAAGGGCCCCAGCCCCGCCGGCCCCACTAGCCCCGCCAGCCCCGCCAGCCCCACCAGCCCTCACACCCAAGACTGGAGTAAATGAAAACACAGTTGTCTCTGCAGGGAAAGATCTCTCTACTTCTCCAAAGCCAAGCCCGATACCAAGTCCTGTTCTGGGGCAAAAGCCAAACGCTAGTCAGTCGCTGCTTGCATGGTGCAGAGAAGTTACAAAAAACTACCGGGGCGTGAAAATCACCAACTTTACCACATCGTGGAGGAATGGCTTATCATTCTGTGCAATTTTACACCACTTTAGACCAGACTTAATCGACTACAAGTCTCTGAATCCTCAAGATATTAAAGAAAACAACAAAAAGGCTTACGATGGATTTGCCAGCATTGGAATTTCCCGTTTACTGGAACCTTCTGATATGGTTTTATTAGCAATCCCTGACAAACTGACTGTTATGACTTATCTCTATCAAATAAGGGCACATTTTAGCGGACAAGAACTAAATGTGGTTCAGATAGAGGAAAACAGCAGTAAGAGCACATATAAAGTTGGAAATTATGAGACAGACACAAACAGTTCTGTGGATCAAGAAAAGTTCTACGCTGAACTCAGCGATCTGAAGCGAGAGCCTGAACCTCACCAGCCTGCCCGTGGGGCTGTGGATCTGCTGTCGCAGGATGACTCTGTGTTTGTAACTGACAGTGGGGTGGGAGAGTCTGAAAGTGAACACCAGACTCCAGATGACCACCTTAGTCCAAGCACAGCCTCCCCTTACTACCGCAGGACTAAGAGTGACACGGAGCCCCAAAAGTCGCAGCAGAGCTCCGCGAGGACTTCAGGGTCTGATGACCCTGGGTTAAGTTCTAGTACTGATTCAGCTCAAGCCCTGGCTTCCTTAGGCAAGAAGAGACTAAAAGCTGAGAACTTGGAACTAAGTGACTTATGTGTCAGTGACAAGAAGAAGGATGTGTCTCCACTCTCAGCTTACGAGCAAAAGCTTCAGACTGTACATGCCAGCAGTGATATGGAACAAGGGAAAATGGAGAAGTCCAGATCTTTAGAATGCAGATTGGATGGTGAATTGGCTATCACAAAGCCAAATGTATCCTCTCCTTCTAAACTTGGATATAACAGAGACACAGACTTCACGAAAAAACCATGTGCTTCTCTGAGGCAGATAGAGTCTGATCCAGATGCAGACAAGAGCACTTTAAATCATGCAGACCACCCCAACAAAGCAGTTCAGCACCGAATGTTGTCCAGACAAGAGGAGCTGAAAGAAAGAGCGAGAGTTCTGCTTGAGCAAGCAAGAAGAGATGCTGCTTTCAAGGTGGGGAGCAAGCATGGTGGCAGCGCAGCCCCTGCGCTCTGCAGCAGACAGCTAAATGATCAGCAAGATGAAGAGAGACGTCGGCAGCTGAGAGAGAGGGCTCGTCAGCTAATAGCAGAAGCTCGATGCGGAGTGAAGATGTCAGAACTTCCCAGCTATGGTGAAATGGCTGCAGAAAAGTTGAAAGAAAGGTCAAAGGCATCTGGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCGATGCGGAGTGAAGATGT
59.18
ACTGTGGAGTCTGTACACCT
57.62
179
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCCAGCTATGGTGAAATGGC
58.96
ACTGTGGAGTCTGTACACCT
57.62
151
PCR template sequence:
CTAATAGCAGAAGCTCGATGCGGAGTGAAGATGTCAGAACTTCCCAGCTATGGTGAAATGGCTGCAGAAAAGTTGAAAGAAAGGTCAAAGGCATCTGGAGAACCAATCACTGAAACAACTTCACCTAAAAAACCAGAAGAATCTTTTTATAATAACAGCTGTAATCCCTTTAAAGGTGTACAGACTCCACAGTATTTGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
20
Interacting miRNAs (#binding sites)
mmu-miR-223-3p(2), mmu-miR-541-5p(2), mmu-miR-20b-5p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-32-5p(1), mmu-miR-429-3p(1), mmu-miR-200c-3p(1), mmu-miR-322-5p(1), mmu-miR-217-5p(1), mmu-miR-138-5p(1), mmu-miR-136-5p(1), mmu-miR-363-3p(1), mmu-miR-29b-1-5p(1), mmu-miR-218-5p(1), mmu-miR-382-5p(1), mmu-miR-383-5p(1), mmu-miR-224-5p(1), mmu-miR-335-3p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
AAGGCATCTGGAGAACCAATCACTGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEHBP1_1860_ORF_1
0.9615
0.152239868
1
3591
ORF sequence:
ATGATAAAGGATTCTCCACCCCAGTCTACACGAAGAAAAAACCTAAGACCTGTAGACATGAGCAAGTACCTCTATGCTGATAGTTCAAAGAGTGAAGAGGAACTGGATGAATCAAATCCTTTTTATGAGCCTAAGCCAACTTCTCCAAATAATTTGGTCAATACAGTTCAAGAAGGGGAAACTGAACGGAGAGTGAAAAGAAGGGCCCCAGCCCCGCCGGCCCCACTAGCCCCGCCAGCCCCGCCAGCCCCACCAGCCCTCACACCCAAGACTGGAGTAAATGAAAACACAGTTGTCTCTGCAGGGAAAGATCTCTCTACTTCTCCAAAGCCAAGCCCGATACCAAGTCCTGTTCTGGGGCAAAAGCCAAACGCTAGTCAGTCGCTGCTTGCATGGTGCAGAGAAGTTACAAAAAACTACCGGGGCGTGAAAATCACCAACTTTACCACATCGTGGAGGAATGGCTTATCATTCTGTGCAATTTTACACCACTTTAGACCAGACTTAATCGACTACAAGTCTCTGAATCCTCAAGATATTAAAGAAAACAACAAAAAGGCTTACGATGGATTTGCCAGCATTGGAATTTCCCGTTTACTGGAACCTTCTGATATGGTTTTATTAGCAATCCCTGACAAACTGACTGTTATGACTTATCTCTATCAAATAAGGGCACATTTTAGCGGACAAGAACTAAATGTGGTTCAGATAGAGGAAAACAGCAGTAAGAGCACATATAAAGTTGGAAATTATGAGACAGACACAAACAGTTCTGTGGATCAAGAAAAGTTCTACGCTGAACTCAGCGATCTGAAGCGAGAGCCTGAACCTCACCAGCCTGCCCGTGGGGCTGTGGATCTGCTGTCGCAGGATGACTCTGTGTTTGTAACTGACAGTGGGGTGGGAGAGTCTGAAAGTGAACACCAGACTCCAGATGACCACCTTAGTCCAAGCACAGCCTCCCCTTACTACCGCAGGACTAAGAGTGACACGGAGCCCCAAAAGTCGCAGCAGAGCTCCGCGAGGACTTCAGGGTCTGATGACCCTGGGTTAAGTTCTAGTACTGATTCAGCTCAAGCCCTGGCTTCCTTAGGCAAGAAGAGACTAAAAGCTGAGAACTTGGAACTAAGTGACTTATGTGTCAGTGACAAGAAGAAGGATGTGTCTCCACTCTCAGCTTACGAGCAAAAGCTTCAGACTGTACATGCCAGCAGTGATATGGAACAAGGGAAAATGGAGAAGTCCAGATCTTTAGAATGCAGATTGGATGGTGAATTGGCTATCACAAAGCCAAATGTATCCTCTCCTTCTAAACTTGGATATAACAGAGACACAGACTTCACGAAAAAACCATGTGCTTCTCTGAGGCAGATAGAGTCTGATCCAGATGCAGACAAGAGCACTTTAAATCATGCAGACCACCCCAACAAAGCAGTTCAGCACCGAATGTTGTCCAGACAAGAGGAGCTGAAAGAAAGAGCGAGAGTTCTGCTTGAGCAAGCAAGAAGAGATGCTGCTTTCAAGGTGGGGAGCAAGCATGGTGGCAGCGCAGCCCCTGCGCTCTGCAGCAGACAGCTAAATGATCAGCAAGATGAAGAGAGACGTCGGCAGCTGAGAGAGAGGGCTCGTCAGCTAATAGCAGAAGCTCGATGCGGAGTGAAGATGTCAGAACTTCCCAGCTATGGTGAAATGGCTGCAGAAAAGTTGAAAGAAAGGTCAAAGGCATCTGGAGAACCAATCACTGAAACAACTTCACCTAAAAAACCAGAAGAATCTTTTTATAATAACAGCTGTAATCCCTTTAAAGGTGTACAGACTCCACAGTATTTGAACCCATTTGATGAACCAGAAACTTTTGTAATGATAAAGGATTCTCCACCCCAGTCTACACGAAGAAAAAACCTAAGACCTGTAGACATGAGCAAGTACCTCTATGCTGATAGTTCAAAGAGTGAAGAGGAACTGGATGAATCAAATCCTTTTTATGAGCCTAAGCCAACTTCTCCAAATAATTTGGTCAATACAGTTCAAGAAGGGGAAACTGAACGGAGAGTGAAAAGAAGGGCCCCAGCCCCGCCGGCCCCACTAGCCCCGCCAGCCCCGCCAGCCCCACCAGCCCTCACACCCAAGACTGGAGTAAATGAAAACACAGTTGTCTCTGCAGGGAAAGATCTCTCTACTTCTCCAAAGCCAAGCCCGATACCAAGTCCTGTTCTGGGGCAAAAGCCAAACGCTAGTCAGTCGCTGCTTGCATGGTGCAGAGAAGTTACAAAAAACTACCGGGGCGTGAAAATCACCAACTTTACCACATCGTGGAGGAATGGCTTATCATTCTGTGCAATTTTACACCACTTTAGACCAGACTTAATCGACTACAAGTCTCTGAATCCTCAAGATATTAAAGAAAACAACAAAAAGGCTTACGATGGATTTGCCAGCATTGGAATTTCCCGTTTACTGGAACCTTCTGATATGGTTTTATTAGCAATCCCTGACAAACTGACTGTTATGACTTATCTCTATCAAATAAGGGCACATTTTAGCGGACAAGAACTAAATGTGGTTCAGATAGAGGAAAACAGCAGTAAGAGCACATATAAAGTTGGAAATTATGAGACAGACACAAACAGTTCTGTGGATCAAGAAAAGTTCTACGCTGAACTCAGCGATCTGAAGCGAGAGCCTGAACCTCACCAGCCTGCCCGTGGGGCTGTGGATCTGCTGTCGCAGGATGACTCTGTGTTTGTAACTGACAGTGGGGTGGGAGAGTCTGAAAGTGAACACCAGACTCCAGATGACCACCTTAGTCCAAGCACAGCCTCCCCTTACTACCGCAGGACTAAGAGTGACACGGAGCCCCAAAAGTCGCAGCAGAGCTCCGCGAGGACTTCAGGGTCTGATGACCCTGGGTTAAGTTCTAGTACTGATTCAGCTCAAGCCCTGGCTTCCTTAGGCAAGAAGAGACTAAAAGCTGAGAACTTGGAACTAAGTGACTTATGTGTCAGTGACAAGAAGAAGGATGTGTCTCCACTCTCAGCTTACGAGCAAAAGCTTCAGACTGTACATGCCAGCAGTGATATGGAACAAGGGAAAATGGAGAAGTCCAGATCTTTAGAATGCAGATTGGATGGTGAATTGGCTATCACAAAGCCAAATGTATCCTCTCCTTCTAAACTTGGATATAACAGAGACACAGACTTCACGAAAAAACCATGTGCTTCTCTGAGGCAGATAGAGTCTGATCCAGATGCAGACAAGAGCACTTTAAATCATGCAGACCACCCCAACAAAGCAGTTCAGCACCGAATGTTGTCCAGACAAGAGGAGCTGAAAGAAAGAGCGAGAGTTCTGCTTGAGCAAGCAAGAAGAGATGCTGCTTTCAAGGTGGGGAGCAAGCATGGTGGCAGCGCAGCCCCTGCGCTCTGCAGCAGACAGCTAAATGATCAGCAAGATGAAGAGAGACGTCGGCAGCTGAGAGAGAGGGCTCGTCAGCTAATAGCAGAAGCTCGATGCGGAGTGAAGATGTCAGAACTTCCCAGCTATGGTGAAATGGCTGCAGAAAAGTTGAAAGAAAGGTCAAAGGCATCTGGA
Peptide sequence:
MIKDSPPQSTRRKNLRPVDMSKYLYADSSKSEEELDESNPFYEPKPTSPNNLVNTVQEGETERRVKRRAPAPPAPLAPPAPPAPPALTPKTGVNENTVVSAGKDLSTSPKPSPIPSPVLGQKPNASQSLLAWCREVTKNYRGVKITNFTTSWRNGLSFCAILHHFRPDLIDYKSLNPQDIKENNKKAYDGFASIGISRLLEPSDMVLLAIPDKLTVMTYLYQIRAHFSGQELNVVQIEENSSKSTYKVGNYETDTNSSVDQEKFYAELSDLKREPEPHQPARGAVDLLSQDDSVFVTDSGVGESESEHQTPDDHLSPSTASPYYRRTKSDTEPQKSQQSSARTSGSDDPGLSSSTDSAQALASLGKKRLKAENLELSDLCVSDKKKDVSPLSAYEQKLQTVHASSDMEQGKMEKSRSLECRLDGELAITKPNVSSPSKLGYNRDTDFTKKPCASLRQIESDPDADKSTLNHADHPNKAVQHRMLSRQEELKERARVLLEQARRDAAFKVGSKHGGSAAPALCSRQLNDQQDEERRRQLRERARQLIAEARCGVKMSELPSYGEMAAEKLKERSKASGEPITETTSPKKPEESFYNNSCNPFKGVQTPQYLNPFDEPETFVMIKDSPPQSTRRKNLRPVDMSKYLYADSSKSEEELDESNPFYEPKPTSPNNLVNTVQEGETERRVKRRAPAPPAPLAPPAPPAPPALTPKTGVNENTVVSAGKDLSTSPKPSPIPSPVLGQKPNASQSLLAWCREVTKNYRGVKITNFTTSWRNGLSFCAILHHFRPDLIDYKSLNPQDIKENNKKAYDGFASIGISRLLEPSDMVLLAIPDKLTVMTYLYQIRAHFSGQELNVVQIEENSSKSTYKVGNYETDTNSSVDQEKFYAELSDLKREPEPHQPARGAVDLLSQDDSVFVTDSGVGESESEHQTPDDHLSPSTASPYYRRTKSDTEPQKSQQSSARTSGSDDPGLSSSTDSAQALASLGKKRLKAENLELSDLCVSDKKKDVSPLSAYEQKLQTVHASSDMEQGKMEKSRSLECRLDGELAITKPNVSSPSKLGYNRDTDFTKKPCASLRQIESDPDADKSTLNHADHPNKAVQHRMLSRQEELKERARVLLEQARRDAAFKVGSKHGGSAAPALCSRQLNDQQDEERRRQLRERARQLIAEARCGVKMSELPSYGEMAAEKLKERSKASG
IRESfinder result
IRESfinder score
0.540038161
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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