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mmu_circEfl1_185
Basic information of circular RNA
PanCircBase ID:
mmu_circEfl1_185
mm39_Chr. Position ID:
chr7|82421664|82421849|+
Gene name:
Efl1
Transcript:
ENSMUST00000179489.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
19
Exon/intron offsets:
0
Genomic length:
185
Exon/intron sizes in nt:
185
Flanking exon/introns:
chr7:82412622-82421664|chr7:82421849-82426698
Splice length:
185
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GCCGAGTCTATGCCGTGCTGTCAAAGCGAGAAGGTCGAGTGCTTCAGGAAGAGATGAAGGAAGGGACGGACATGTTCATCATCAAGGCTGTGCTGCCTGTAGCGGAAAGCTTTGGCTTTGCGGATGAGATCAGGAAGAGGACCAGCGGACTGGCCAGCCCACAGCTGGTGTTCAGCCACTGGGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GATCAGGAAGAGGACCAGCG
59.9
TGAACATGTCCGTCCCTTCC
59.68
136
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGCTTTGCGGATGAGATCA
59.75
TGAACATGTCCGTCCCTTCC
59.68
151
PCR template sequence:
TGCCTGTAGCGGAAAGCTTTGGCTTTGCGGATGAGATCAGGAAGAGGACCAGCGGACTGGCCAGCCCACAGCTGGTGTTCAGCCACTGGGAGGCCGAGTCTATGCCGTGCTGTCAAAGCGAGAAGGTCGAGTGCTTCAGGAAGAGATGAAGGAAGGGACGGACATGTTCATCATCAAGGCTGTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-205-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CAGCCACTGGGAGGCCGAGTCTATGC
siRNA sets
CATAGACTCGGCCTCCCAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEFL1_185_ORF_1
1.139
-0.266427346
0.054216948
189
ORF sequence:
ATGAGATCAGGAAGAGGACCAGCGGACTGGCCAGCCCACAGCTGGTGTTCAGCCACTGGGAGGCCGAGTCTATGCCGTGCTGTCAAAGCGAGAAGGTCGAGTGCTTCAGGAAGAGATGAAGGAAGGGACGGACATGTTCATCATCAAGGCTGTGCTGCCTGTAGCGGAAAGCTTTGGCTTTGCGGATGA
Peptide sequence:
MRSGRGPADWPAHSWCSATGRPSLCRAVKARRSSASGRDEGRDGHVHHQGCAACSGKLWLCG
IRESfinder result
IRESfinder score
0.177886706
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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