top of page
mmu_circEef1g_351
Basic information of circular RNA
PanCircBase ID:
mmu_circEef1g_351
mm39_Chr. Position ID:
chr19|8946525|8947351|+
Gene name:
Eef1g
Transcript:
ENSMUST00000052248.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
3,4,5
Exon/intron offsets:
0,202,682
Genomic length:
826
Exon/intron sizes in nt:
6,41,43,144
Flanking exon/introns:
chr19:8945738-8946525|chr19:8947351-8949870
Splice length:
351
Other database IDs:
CIRCpedia: MMU_CIRCpedia_226464
Human Ortholog IDs:
Mature spliced Sequence:
GTTCCAGCATTTGAGGGTGATGATGGATTCTGTGTGTTTGAGAGCAATGCCATTGCCTATTATGTAAGCAATGAGGAGCTGCGAGGAAGTACGCCAGAGGCGGCAGCTCAGGTGGTGCAGTGGGTCAGCTTTGCTGACAGTGACATCGTTCCTCCAGCTAGTACCTGGGTGTTCCCCACCTTGGGCATTATGCACCACAACAAACAGGCCACTGAAAATGCGAAAGAGGAGGTGAAGCGAATCCTGGGGCTGCTGGACACTCACTTGAAGACTCGAACTTTTCTAGTGGGCGAGCGAGTGACACTGGCTGATATTACAGTTGTCTGTACCCTGTTGTGGCTCTATAAACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTTTCTAGTGGGCGAGCGAG
60.11
GTACTTCCTCGCAGCTCCTC
59.9
165
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTTTTCTAGTGGGCGAGCGA
60.11
GTACTTCCTCGCAGCTCCTC
59.9
166
PCR template sequence:
GCTGGACACTCACTTGAAGACTCGAACTTTTCTAGTGGGCGAGCGAGTGACACTGGCTGATATTACAGTTGTCTGTACCCTGTTGTGGCTCTATAAACAGGTTCCAGCATTTGAGGGTGATGATGGATTCTGTGTGTTTGAGAGCAATGCCATTGCCTATTATGTAAGCAATGAGGAGCTGCGAGGAAGTACGCCAGAGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-137-3p(1), mmu-miR-5101(1)
siRNAs for circular RNA silencing
Junction sequence
GCTCTATAAACAGGTTCCAGCATTTG
siRNA sets
AATGCTGGAACCTGTTTAT, AAATGCTGGAACCTGTTTA, TGGAACCTGTTTATAGAGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEEF1G_351_ORF_1
1.1797
0.331167969
0.879772736
513
ORF sequence:
ATGCACCACAACAAACAGGCCACTGAAAATGCGAAAGAGGAGGTGAAGCGAATCCTGGGGCTGCTGGACACTCACTTGAAGACTCGAACTTTTCTAGTGGGCGAGCGAGTGACACTGGCTGATATTACAGTTGTCTGTACCCTGTTGTGGCTCTATAAACAGGTTCCAGCATTTGAGGGTGATGATGGATTCTGTGTGTTTGAGAGCAATGCCATTGCCTATTATGTAAGCAATGAGGAGCTGCGAGGAAGTACGCCAGAGGCGGCAGCTCAGGTGGTGCAGTGGGTCAGCTTTGCTGACAGTGACATCGTTCCTCCAGCTAGTACCTGGGTGTTCCCCACCTTGGGCATTATGCACCACAACAAACAGGCCACTGAAAATGCGAAAGAGGAGGTGAAGCGAATCCTGGGGCTGCTGGACACTCACTTGAAGACTCGAACTTTTCTAGTGGGCGAGCGAGTGACACTGGCTGATATTACAGTTGTCTGTACCCTGTTGTGGCTCTATAAACAG
Peptide sequence:
MHHNKQATENAKEEVKRILGLLDTHLKTRTFLVGERVTLADITVVCTLLWLYKQVPAFEGDDGFCVFESNAIAYYVSNEELRGSTPEAAAQVVQWVSFADSDIVPPASTWVFPTLGIMHHNKQATENAKEEVKRILGLLDTHLKTRTFLVGERVTLADITVVCTLLWLYKQ
IRESfinder result
IRESfinder score
0.416807206
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page