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mmu_circEea1_249
Basic information of circular RNA
PanCircBase ID:
mmu_circEea1_249
mm39_Chr. Position ID:
chr10|95825423|95826327|+
Gene name:
Eea1
Transcript:
ENSMUST00000218088.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
4,5,6
Exon/intron offsets:
0,693,838
Genomic length:
904
Exon/intron sizes in nt:
128,55,66
Flanking exon/introns:
chr10:95814148-95825423|chr10:95826327-95829285
Splice length:
249
Other database IDs:
circBase: mmu_circ_0002601, CIRCpedia: MMU_CIRCpedia_35232, circAtlas: mmu-Eea1_0002
circAtlas: hsa-EEA1_0004, CIRCpedia: HSA_CIRCpedia_166869
Human Ortholog IDs:
Mature spliced Sequence:
GGTTTCATATGTCCCCAGTGTATGAAATCCCTTGGCTCTGCTGATGAGCTTTTCAAGCACTATCAGGCCGTTCATGATGCTGGTAATGACTCGGGTCATGGTGGAGAGGCCGGTCTTGCTTTGACACGAGATGACATAACACTACTCAGACAAGAGGTCCAAGACCTACAGGCCTCACTTAAGGAAGAAAAATGGTACTCAGAAGAATTAAAGAAAGAATTAGAAAAATATCAAGGGCTACAGCAGCAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCAAGACCTACAGGCCTCA
59.88
AGCATCATGAACGGCCTGAT
59.82
173
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCAAGACCTACAGGCCTCAC
59.75
AGCATCATGAACGGCCTGAT
59.82
172
PCR template sequence:
ACAAGAGGTCCAAGACCTACAGGCCTCACTTAAGGAAGAAAAATGGTACTCAGAAGAATTAAAGAAAGAATTAGAAAAATATCAAGGGCTACAGCAGCAAGGTTTCATATGTCCCCAGTGTATGAAATCCCTTGGCTCTGCTGATGAGCTTTTCAAGCACTATCAGGCCGTTCATGATGCTGGTAATGACTCGGGTCATG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-541-5p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GCTACAGCAGCAAGGTTTCATATGTC
siRNA sets
TATGAAACCTTGCTGCTGT, ACATATGAAACCTTGCTGC, CATATGAAACCTTGCTGCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCEEA1_249_ORF_1
1.1645
0.129061004
0.706961884
477
ORF sequence:
ATGAAATCCCTTGGCTCTGCTGATGAGCTTTTCAAGCACTATCAGGCCGTTCATGATGCTGGTAATGACTCGGGTCATGGTGGAGAGGCCGGTCTTGCTTTGACACGAGATGACATAACACTACTCAGACAAGAGGTCCAAGACCTACAGGCCTCACTTAAGGAAGAAAAATGGTACTCAGAAGAATTAAAGAAAGAATTAGAAAAATATCAAGGGCTACAGCAGCAAGGTTTCATATGTCCCCAGTGTATGAAATCCCTTGGCTCTGCTGATGAGCTTTTCAAGCACTATCAGGCCGTTCATGATGCTGGTAATGACTCGGGTCATGGTGGAGAGGCCGGTCTTGCTTTGACACGAGATGACATAACACTACTCAGACAAGAGGTCCAAGACCTACAGGCCTCACTTAAGGAAGAAAAATGGTACTCAGAAGAATTAAAGAAAGAATTAGAAAAATATCAAGGGCTACAGCAGCAA
Peptide sequence:
MKSLGSADELFKHYQAVHDAGNDSGHGGEAGLALTRDDITLLRQEVQDLQASLKEEKWYSEELKKELEKYQGLQQQGFICPQCMKSLGSADELFKHYQAVHDAGNDSGHGGEAGLALTRDDITLLRQEVQDLQASLKEEKWYSEELKKELEKYQGLQQQ
IRESfinder result
IRESfinder score
0.381897142
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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